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Accurate mass analysis of phosphoramidites by electrospray mass spectrometry.
Kupihár, Z; Timár, Z; Dellinger, D J; Caruthers, M H.
Nucleosides Nucleotides Nucleic Acids ; 24(5-7): 663-6, 2005.
Article in English | MEDLINE | ID: mdl-16248009

ABSTRACT

A method of accurate mass determination of phosphoramidites is described. The commonly used methanol/water/acid system was replaced by LiCl-containing acetonitrile and the concentrations of LiCl, poly(ethylene glycol), and phosphoramidite samples were optimized.


Subject(s)
Molecular Biology/methods , Organophosphorus Compounds/pharmacology , Spectrometry, Mass, Electrospray Ionization/methods , Acetonitriles/chemistry , Acetonitriles/pharmacology , Lithium Chloride/chemistry , Lithium Chloride/pharmacology , Macromolecular Substances , Mass Spectrometry/methods , Models, Chemical , Organophosphorus Compounds/chemistry , Polyethylene Glycols/chemistry , Polymers/chemistry , Reproducibility of Results
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