ABSTRACT
Various types of collagen (I, III, IV, V) were identified in normal and varicose human saphenous veins using pepsin digestion and cyanogen bromide digestion followed by SDS-polyacrylamide gel electrophoresis. Varicose veins were found to have a higher collagen content than normal veins. This is consistent with the morphological fibrosis which has regularly been described. No essential differences were found in the collagen composition of dilated and apparently healthy portions of varicose veins.
Subject(s)
Collagen/metabolism , Saphenous Vein/metabolism , Varicose Veins/metabolism , Adult , Aged , Aged, 80 and over , Collagen/classification , Collagen/isolation & purification , Electrophoresis, Polyacrylamide Gel , Humans , Middle AgedABSTRACT
Two methods of skin biopsy and cholesterol assay are compared. Skin cholesterol assay after punch-biopsy (micro-method) is better than the macro-method: skin cholesterol assay after surgical removal of a sample. Skin biopsy which was shown to give information on the arterial wall thus becomes an easily accessible routine technique.