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1.
Genomics ; 115(2): 110580, 2023 03.
Article in English | MEDLINE | ID: mdl-36792020

ABSTRACT

Bloodstream infections are a major cause of morbidity and mortality worldwide. Early administration of appropriate antimicrobial therapy can improve patient survival and prevent antimicrobial resistance (AMR). Whole genome sequencing (WGS) can provide information for pathogen identification, AMR prediction and sequence typing earlier than current phenotypic diagnostic methods. WGS was performed on 97 clinical blood specimens and matched culture isolate pairs. Specimen/isolate pairs were MLST sequence-typed and further characterization was performed on Streptococcus species. WGS correctly identified 91.7% of clinical specimens and 93.2% of matched isolates representing 35 different microbial species. MLST types were assigned for 89.9% of matched cultures and 21.7% of blood specimens, with higher success for blood culture specimens extracted within 3 days (52% characterized) than 7 days (9.3%). This study demonstrates the potential use of WGS for identification and characterization of pathogens directly from blood culture specimens to facilitate timely initiation of appropriate antimicrobial therapies.


Subject(s)
Blood Culture , Genome, Bacterial , Humans , Multilocus Sequence Typing , Bacteria , Whole Genome Sequencing , Drug Resistance, Bacterial/genetics , Anti-Bacterial Agents/pharmacology
2.
J Antimicrob Chemother ; 75(12): 3485-3490, 2020 12 01.
Article in English | MEDLINE | ID: mdl-32830242

ABSTRACT

BACKGROUND: The incidence of antimicrobial-resistant Neisseria gonorrhoeae (GC) is rising in Canada; however, antimicrobial resistance (AMR) surveillance data are unavailable for infections diagnosed directly from clinical specimens by nucleic acid amplification tests (NAATs), representing over 80% of diagnoses. We developed a set of 10 improved molecular assays for surveillance of GC-AMR and prediction of susceptibilities in NAAT specimens. METHODS: Multiplex real-time PCR (RT-PCR) assays were developed to detect SNPs associated with cephalosporin (ponA, porB, mtrR -35delA, penA A311V, penA A501, N513Y, G545S), ciprofloxacin (gyrA S91, parC D86/S87/S88) and azithromycin [23S (A2059G, C2611T), mtrR meningitidis-like promoter] resistance. The assays were validated on 127 gonococcal isolates, 51 non-gonococcal isolates and 50 NAATs with matched culture isolates. SNPs determined from the assay were compared with SNPs determined from in silico analysis of WGS data. MICs were determined for culture isolates using the agar dilution method. RESULTS: SNP analysis of the 50 NAAT specimens had 96% agreement with the matched culture RT-PCR analysis. When compared with MICs, presence of penA A311V or penA A501 and two or more other SNPs correlated with decreased susceptibility and presence of three or more other SNPs correlated with intermediate susceptibility to cephalosporins; presence of any associated SNP correlated with ciprofloxacin or azithromycin resistance. NAAT-AMR predictions correlated with matched-culture cephalosporin, ciprofloxacin and azithromycin MICs at 94%, 100% and 98%, respectively. CONCLUSIONS: We expanded molecular tests for N. gonorrhoeae AMR prediction by adding new loci and multiplexing reactions to improve surveillance where culture isolates are unavailable.


Subject(s)
Gonorrhea , Neisseria gonorrhoeae , Anti-Bacterial Agents/pharmacology , Azithromycin/pharmacology , Canada , Cephalosporins/pharmacology , Ciprofloxacin/pharmacology , Drug Resistance, Bacterial , Humans , Microbial Sensitivity Tests , Neisseria gonorrhoeae/genetics , Real-Time Polymerase Chain Reaction
3.
J Clin Microbiol ; 55(5): 1454-1468, 2017 05.
Article in English | MEDLINE | ID: mdl-28228492

ABSTRACT

A curated Web-based user-friendly sequence typing tool based on antimicrobial resistance determinants in Neisseria gonorrhoeae was developed and is publicly accessible (https://ngstar.canada.ca). The N. gonorrhoeae Sequence Typing for Antimicrobial Resistance (NG-STAR) molecular typing scheme uses the DNA sequences of 7 genes (penA, mtrR, porB, ponA, gyrA, parC, and 23S rRNA) associated with resistance to ß-lactam antimicrobials, macrolides, or fluoroquinolones. NG-STAR uses the entire penA sequence, combining the historical nomenclature for penA types I to XXXVIII with novel nucleotide sequence designations; the full mtrR sequence and a portion of its promoter region; portions of ponA, porB, gyrA, and parC; and 23S rRNA sequences. NG-STAR grouped 768 isolates into 139 sequence types (STs) (n = 660) consisting of 29 clonal complexes (CCs) having a maximum of a single-locus variation, and 76 NG-STAR STs (n = 109) were identified as unrelated singletons. NG-STAR had a high Simpson's diversity index value of 96.5% (95% confidence interval [CI] = 0.959 to 0.969). The most common STs were NG-STAR ST-90 (n = 100; 13.0%), ST-42 and ST-91 (n = 45; 5.9%), ST-64 (n = 44; 5.72%), and ST-139 (n = 42; 5.5%). Decreased susceptibility to azithromycin was associated with NG-STAR ST-58, ST-61, ST-64, ST-79, ST-91, and ST-139 (n = 156; 92.3%); decreased susceptibility to cephalosporins was associated with NG-STAR ST-90, ST-91, and ST-97 (n = 162; 94.2%); and ciprofloxacin resistance was associated with NG-STAR ST-26, ST-90, ST-91, ST-97, ST-150, and ST-158 (n = 196; 98.0%). All isolates of NG-STAR ST-42, ST-43, ST-63, ST-81, and ST-160 (n = 106) were susceptible to all four antimicrobials. The standardization of nomenclature associated with antimicrobial resistance determinants through an internationally available database will facilitate the monitoring of the global dissemination of antimicrobial-resistant N. gonorrhoeae strains.


Subject(s)
Anti-Bacterial Agents/pharmacology , Drug Resistance, Multiple, Bacterial/genetics , Multilocus Sequence Typing/methods , Neisseria gonorrhoeae/classification , Neisseria gonorrhoeae/drug effects , Amino Acid Sequence , Azithromycin/pharmacology , Cephalosporins/pharmacology , Fluoroquinolones/pharmacology , Gonorrhea/epidemiology , Gonorrhea/microbiology , Humans , Neisseria gonorrhoeae/genetics , Neisseria gonorrhoeae/isolation & purification
4.
Can Commun Dis Rep ; 42(4): 74-82, 2016 Apr 07.
Article in English | MEDLINE | ID: mdl-29770008

ABSTRACT

BACKGROUND: Northern populations were at a high risk of developing invasive bacterial diseases (IBDs). Since the last published study that described IBDs in Northern Canada, a number of vaccines against some bacterial pathogens have been introduced into the routine childhood immunization schedule. OBJECTIVE: To describe the epidemiology of IBDs in Northern Canada from 2006 to 2013. METHODS: Data for 5 IBDs (invasive pneumococcal disease (IPD), invasive Haemophilus influenzae disease (Hi), invasive Group A streptococcal disease (iGAS), invasive meningococcal disease (IMD) and invasive Group B streptococcal disease (GBS)) were extracted from the International Circumpolar Surveillance (ICS) program and the Canadian Notifiable Diseases Surveillance System. Incidence rates were calculated per 100,000 population per year. RESULTS: During the study period, the incidence rates of IPD ranged from 16.84-30.97, iGAS 2.70-17.06, Hi serotype b 0-2.78, Hi non-b type 2.73-8.53, and IMD 0-3.47. Except for IMD and GBS, the age-standardized incidence rates of other diseases in Northern Canada were 2.6-10 times higher than in the rest of Canada. Over the study period, rates decreased for IPD (p=0.04), and iGAS (p=0.01), and increased for Hi type a (Hia) (p=0.004). Among IPD cases, the proportion of pneumococcal conjugate vaccine (PCV)7 serotypes decreased (p=0.0004) over the study period. Among Hi cases, 69.8% were Hia and 71.6% of these were in children under than 5 years. Of 13 IMD cases, 8 were serogroup B and 2 of them died. CONCLUSION: Northern population in Canada, especially infants and seniors among First Nations and Inuit, are at a high risk of IPD, Hi and iGAS. Hia is the predominant serotype in Northern Canada.

5.
J Clin Microbiol ; 53(11): 3606-8, 2015 Nov.
Article in English | MEDLINE | ID: mdl-26292300

ABSTRACT

We developed a real-time PCR assay to detect single nucleotide polymorphisms associated with ciprofloxacin resistance in specimens submitted for nucleic acid amplification testing (NAAT). All three single nucleotide polymorphism (SNP) targets produced high sensitivity and specificity values. The presence of ≥2 SNPs was sufficient to predict ciprofloxacin resistance in an organism.


Subject(s)
Anti-Bacterial Agents/pharmacology , Ciprofloxacin/pharmacology , Drug Resistance, Bacterial/genetics , Neisseria gonorrhoeae/drug effects , Neisseria gonorrhoeae/genetics , Nucleic Acid Amplification Techniques/methods , Canada , Cross Reactions , DNA Gyrase/genetics , DNA Topoisomerase IV/genetics , Gonorrhea/diagnosis , Gonorrhea/microbiology , Humans , Microbial Sensitivity Tests , Neisseria gonorrhoeae/isolation & purification , Polymerase Chain Reaction/methods , Polymorphism, Single Nucleotide/genetics , Sensitivity and Specificity
6.
J Clin Microbiol ; 53(7): 2042-8, 2015 Jul.
Article in English | MEDLINE | ID: mdl-25878350

ABSTRACT

The incidence of antimicrobial-resistant Neisseria gonorrhoeae continues to rise in Canada; however, antimicrobial resistance data are lacking for approximately 70% of gonorrhea infections that are diagnosed directly from clinical specimens by nucleic acid amplification tests (NAATs). We developed a molecular assay for surveillance use to detect mutations in genes associated with decreased susceptibility to cephalosporins that can be applied to both culture isolates and clinical samples. Real-time PCR assays were developed to detect single nucleotide polymorphisms (SNPs) in ponA, mtrR, penA, porB, and one N. gonorrhoeae-specific marker (porA). We tested the real-time PCR assay with 252 gonococcal isolates, 50 nongonococcal isolates, 24 N. gonorrhoeae-negative NAAT specimens, and 34 N. gonorrhoeae-positive NAAT specimens. Twenty-four of the N. gonorrhoeae-positive NAAT specimens had matched culture isolates. Assay results were confirmed by comparison with whole-genome sequencing data. For 252 N. gonorrhoeae strains, the agreement between the DNA sequence and real-time PCR was 100% for porA, ponA, and penA, 99.6% for mtrR, and 95.2% for porB. The presence of ≥2 SNPs correlated with decreased susceptibility to ceftriaxone (sensitivities of >98%) and cefixime (sensitivities of >96%). Of 24 NAAT specimens with matched cultures, the agreement between the DNA sequence and real-time PCR was 100% for porB, 95.8% for ponA and mtrR, and 91.7% for penA. We demonstrated the utility of a real-time PCR assay for sensitive detection of known markers for the decreased susceptibility to cephalosporins in N. gonorrhoeae. Preliminary results with clinical NAAT specimens were also promising, as they correlated well with bacterial culture results.


Subject(s)
Anti-Bacterial Agents/pharmacology , Cephalosporins/pharmacology , Drug Resistance, Bacterial , Genetic Markers , Genotyping Techniques/methods , Neisseria gonorrhoeae/drug effects , Neisseria gonorrhoeae/genetics , Canada , Female , Genes, Bacterial , Gonorrhea/microbiology , Humans , Male , Microbiological Techniques/methods , Polymorphism, Single Nucleotide , Real-Time Polymerase Chain Reaction/methods , Sensitivity and Specificity
7.
Epidemiol Infect ; 138(10): 1414-26, 2010 Oct.
Article in English | MEDLINE | ID: mdl-20149265

ABSTRACT

Isolation rates in Canada of Salmonella enterica serovar Typhi increased from 0.29 to 0.55 isolations/100,000 population during 2000-2006. Although no ciprofloxacin resistance was detected, nalidixic acid resistance increased from 41% to 80%. Multidrug-resistant S. Typhi represented 18% of the strains tested. Pulsed-field gel electrophoresis (PFGE) analysis of 222 isolates resulted in 91 distinct patterns clustering into four major genetic similarity groups. The five most frequently occurring PFGE patterns accounted for 46% of the isolates. Drug-resistant isolates predominantly occurred in one PFGE similarity group. There were 39 phage types identified in 826 isolates analysed with 60% described by five phage types; 134 were untypable. The phage types associated with multidrug resistance were phage types 53, B1, D1, E1, E9, G3 and M1. Improved integration of epidemiological and laboratory case data will facilitate the protection of public health in Canada during an era of increasing travel and globalization.


Subject(s)
Bacteriophage Typing , DNA Fingerprinting , Drug Resistance, Bacterial , Salmonella typhi/classification , Salmonella typhi/drug effects , Typhoid Fever/epidemiology , Typhoid Fever/microbiology , Adolescent , Adult , Aged , Aged, 80 and over , Anti-Bacterial Agents/pharmacology , Canada/epidemiology , Child , Child, Preschool , Cluster Analysis , DNA, Bacterial/genetics , Electrophoresis, Gel, Pulsed-Field , Female , Genotype , Humans , Infant , Infant, Newborn , Male , Microbial Sensitivity Tests , Middle Aged , Salmonella typhi/isolation & purification , Young Adult
9.
J Clin Microbiol ; 38(6): 2403-6, 2000 Jun.
Article in English | MEDLINE | ID: mdl-10835016

ABSTRACT

A major Canada-wide outbreak of gastroenteritis due to Salmonella enterica serotype Enteritidis phage type (PT) 8 occurred in 1998, and this was traced to contaminated cheese in a commercial lunch pack product. Phage typing and pulsed-field gel electrophoresis linked the clinical and cheese isolates of serotype Enteritidis but failed to differentiate outbreak from nonoutbreak PT 8 strains. Further differentiation was made by biotyping based on melibiose fermentation.


Subject(s)
Cheese/microbiology , Disease Outbreaks , Gastroenteritis/epidemiology , Salmonella Food Poisoning/epidemiology , Salmonella enteritidis/classification , Bacterial Typing Techniques , Bacteriophage Typing , Canada/epidemiology , Electrophoresis, Gel, Pulsed-Field , Gastroenteritis/microbiology , Humans , Molecular Epidemiology , Salmonella Food Poisoning/microbiology
10.
Int J Food Microbiol ; 30(3): 325-44, 1996 Jul.
Article in English | MEDLINE | ID: mdl-8854185

ABSTRACT

Salmonella isolates from 295 layer and 294 broiler flocks in Canada were examined to determine resistance to antimicrobial agents, plasmid profiles, biochemical properties, and susceptibility to polyvalent bacteriophages. Except for the high number of strains resistant to spectinomycin (97.8%), the frequency of drug resistance of Salmonella isolates from layer flocks was low. None of 457 isolates from layer flocks was resistant to amikacin or ciprofloxacin, and less than 2% of the strains were resistant to cephalothin, chloramphenicol, cotrimoxazole, gentamicin, kanamycin, neomycin, nitrofurantoin, and/or polymyxin B. About 3% of the strains were resistant to ampicillin, carbenicillin and/or tetracycline, whereas 8% of the strains were resistant to sulfisoxazole. Salmonella anatum var. O15+ and S. typhimurium var. copenhagen strains were resistant to multiple antimicrobial agents. None of 1159 Salmonella strains from broiler flocks was resistant to amikacin, cephalothin, ciprofloxacin or polymyxin B, less than 1% of the strains were resistant to chloramphenicol, 2% were resistant to ampicillin, carbenicillin and/or chloramphenicol; 5-7% were resistant to the aminoglycosides gentamicin, kanamycin and/or neomycin; 6% were resistant to nitrofurantoin; 10% to tetracycline; 14% to sulfisoxazole; and 99% to spectinomycin. A high percentage of S. binza, S. anatum var. O15+, S. schwarzengrund and S. heidelberg strains were resistant to antimicrobial agents. Some of the single or multiple resistances were encoded by conjugative plasmids or by plasmids that were thermosensitive for transfer. Eight percent of S. heidelberg strains did not produce hydrogen sulfide. Ninety-seven percent of the Salmonella strains were susceptible to the lytic effect of polyvalent bacteriophages.


Subject(s)
Food Microbiology , Poultry/microbiology , Salmonella Phages/physiology , Salmonella/drug effects , Animals , Drug Resistance , Plasmids , Salmonella/classification , Salmonella/genetics
11.
Can J Vet Res ; 59(4): 241-8, 1995 Oct.
Article in English | MEDLINE | ID: mdl-8548684

ABSTRACT

A study was conducted to determine the antibiotic resistance and biochemical characteristics of 2690 Salmonella strains belonging to 52 serovars and isolated from environmental and feed samples from 270 turkey flocks in Canada. Resistance of the Salmonella strains to the aminoglycoside antibiotics varied widely; none of the strains were resistant to amikacin, 14.2% were resistant to neomycin, 25.8% were resistant to gentamicin, and 27.7% of the strains were resistant to kanamycin. Most strains (97.6%) were resistant to the aminocyclitol, spectinomycin. Regarding resistance to the beta-lactam antibiotics, 14.3% and 14.4% of the strains were resistant to ampicillin and carbenicillin, respectively, whereas only 5 (0.2%) of the strains were resistant to cephalothin. None of the strains were resistant to the fluoroquinolone ciprofloxacin or to polymyxin B. Resistance to chloramphenicol and nitrofurantoin was found in 2.4% and 7% of the strains, respectively. Only 1.7% of the strains were resistant to the trimethoprimsulfamethoxazole combination, whereas 58.1% were resistant to sulfisoxazole. Thirty-eight percent of the strains were resistant to tetracycline. Salmonella serovars differed markedly in their drug resistance profiles. Biochemical characterization of the Salmonella showed that the S. anatum, S. saintpaul and S. reading serovars could be divided into distinct biotypes.


Subject(s)
Drug Resistance, Microbial , Salmonella Infections, Animal/microbiology , Salmonella/drug effects , Turkeys/microbiology , Animals , Bacterial Typing Techniques/veterinary , Microbial Sensitivity Tests/veterinary , Salmonella/classification , Species Specificity
12.
Can J Vet Res ; 57(4): 281-7, 1993 Oct.
Article in English | MEDLINE | ID: mdl-8269367

ABSTRACT

Virulence of three Canadian poultry strains of Salmonella enteritidis, namely phagetypes (PT) 4, 8 and 13, and one Salmonella heidelberg strain was assessed in orally and intraperitoneally inoculated one-day old chickens and compared to the virulence of a human S. enteritidis PT 4 strain from the United Kingdom (UK). The two PT 4 strains were also compared in orally inoculated adult laying hens. In addition, orally inoculated Balb/c mice were used to evaluate virulence of the above strains and two strains of Salmonella typhimurium containing different plasmids. In orally inoculated one-day old chickens, the UK S. enteritidis PT 4 strain was more virulent than the Canadian PT 4 strain. The UK PT 4 strain was also more virulent and invasive in adult laying hens than the Canadian PT 4 strain. The S. enteritidis PT 8 strain and one S. typhimurium strain isolated from a chicken hatchery were the most virulent for orally inoculated Balb/c mice. This strain of S. typhimurium contained the 60 megadalton plasmid associated with virulence for Balb/c mice which was not present in the S. typhimurium strain isolated from a pig with septicemic disease.


Subject(s)
Chickens/microbiology , Poultry Diseases/microbiology , Salmonella Infections, Animal/microbiology , Salmonella enteritidis/pathogenicity , Animals , Bacteriophage Typing , Cecum/microbiology , Disease Models, Animal , Eggs/microbiology , Female , Gastrointestinal Contents/microbiology , Lethal Dose 50 , Liver/microbiology , Male , Mice , Mice, Inbred BALB C , Salmonella/pathogenicity , Salmonella enteritidis/classification , Spleen/microbiology , Virulence
13.
Can J Vet Res ; 57(3): 176-84, 1993 Jul.
Article in English | MEDLINE | ID: mdl-8358678

ABSTRACT

A study was conducted to characterize 318 Salmonella enteritidis strains that were mainly isolated from poultry and their environment in Canada. Biotype, phagetype (PT), plasmid profile (PP), hybridization with a plasmid-derived virulence sequence probe, antibiotic resistance, outer membrane proteins (OMPs), and lipopolysaccharide (LPS) profiles were determined. Relationships of these properties to one another, and their diagnostic and pathogenic significance were assessed. Biotyping indicated that failure to ferment rhamnose was sometimes useful as a marker for epidemiologically related strains. Phagetyping was the most effective method for subdividing S. enteritidis; it distinguished 12 PTs. Phagetype 13 was occasionally associated with septicemia and mortality in chickens. The strains belonged to 15 PPs. A 36 megadalton (MDa) plasmid was found in 97% of the strains. Only the 36 MDa plasmid hybridized with the probe. Seventeen percent of the strains were drug resistant; all strains were sensitive to ciprofloxacin. Thirty-five of 36 strains possessed the same OMP profile, and 36 of 41 strains contained smooth LPS.


Subject(s)
Poultry Diseases/microbiology , Salmonella Infections, Animal/microbiology , Salmonella Infections/microbiology , Salmonella enteritidis/classification , Animals , Bacterial Outer Membrane Proteins/analysis , Bacterial Typing Techniques/veterinary , Bacteriophage Typing/veterinary , Chickens , Ciprofloxacin/pharmacology , DNA Probes , DNA, Bacterial/analysis , Drug Resistance, Microbial , Humans , Kanamycin Resistance , Lipopolysaccharides/analysis , Nucleic Acid Hybridization , Plasmids , Salmonella enteritidis/drug effects , Salmonella enteritidis/physiology , Trimethoprim, Sulfamethoxazole Drug Combination/pharmacology , Turkeys , Virulence/genetics
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