Control of gastrointestinal nematodes in first season grazing calves by two strategic treatments with eprinomectin.

A study was carried out to evaluate the effects of strategic early-season treatments with eprinomectin on first-season grazing calves exposed to strongyle infections on a naturally contaminated pasture. Two groups of first grazing season (FGS) calves were turned out in mid-May on two plots that were similar with respect to size and herbage infectivity. They grazed separately until housing at the end of October. One of these groups was given eprinomectin pour-on at turnout and 8 weeks later, while the other group served as untreated controls. The results showed that the treatments reduced gastrointestinal strongyle infections throughout the season as evidenced by lower faecal egg counts and serum pepsinogen levels compared with the controls. Furthermore, the results of herbage larval counts and postmortem worm counts in tracer animals demonstrated that the treatment had reduced herbage infectivity on the 'treated' plot. Finally, the chemoprophylactically treated calves had a better weight gain over the duration of the study than the untreated controls.

Evaluation of the persistent efficacy of doramectin and ivermectin injectable against Ostertagia ostertagi and Cooperia oncophora in cattle.

The persistent efficacy of doramectin and ivermectin injectable against moderate and high infection levels of Ostertagia ostertagi and Cooperia oncophora were evaluated in cattle. Calves were allocated to six groups of six animals. On Day 0 animals of Groups I1/I2 and D1/D2 were treated with 0.2mg/kg ivermectin and doramectin injectable, respectively. Animals of the C1, I1 and D1 groups received a daily (moderate) infection of 1000 L3 of O. ostertagi and 1000 L3 of C. oncophora, and animals of the C2, I2 and D2 groups received a daily (high) infection of 10,000 L3 of each species. The animals were infected for 21 days with both species, the infections with C. oncophora and O. ostertagi started from Days 8 and 15 post treatment, respectively. Animals were necropsied on Day 40. The calculation of the persistent activity of ivermectin and doramectin was based on the efficacy against the different developmental and adult stages of both parasites. The present study confirmed that infection levels may influence the duration of persistent efficacy of an anthelmintic. Doramectin had at the moderate infection level a persistent efficacy of at least 35 days against O. ostertagi and at least 28 days against C. oncophora; at the high infection dose persistent efficacy was somewhat shorter i.e. up to 33 days and approximately 28 days, respectively. The duration of persistent efficacy of ivermectin against O. ostertagi at the moderate infection level was between 14 and 25 days, at the high dose level up to 25 days. Persistent efficacy of ivermectin against C. oncophora could, at both infection doses, not be measured, with the present experimental design.

The effect of first season chemoprophylaxis in calves on second season pasture contamination and acquired resistance and resilience to gastrointestinal nematodes.

Two groups of second grazing season cattle, which had been treated with an ivermectin-sustained-release bolus (ISRB) in their first grazing season, were monitored during consecutive years (1995 and 1996) on the same second grazing season pasture. In the preceding year (1994), this pasture had been grazed by yearlings that had not received chemoprophylaxis in their first grazing season. The objectives of the study were, firstly, to investigate whether the chemoprophylactic-treated yearlings were less resistant to gastrointestinal nematodes upon subsequent exposure, and hence excreted more strongyle eggs compared to the control yearlings; secondly, whether an increased susceptibility of the previously treated animals resulted in a yearly increase of the pasture infestation on the second grazing season pasture; and finally, whether this affected the second year weight gain of the animals. In 1996, the yearlings that had been chemoprophylactic-treated in 1995 excreted higher numbers of nematode eggs, compared to the previously 'untreated' yearlings. In addition, the proportion of Cooperia larvae was markedly higher in the faecal cultures from the chemoprophylactic treated-animals, suggesting a negative effect of preventive treatment with an ISRB on the acquired resistance of the animals. However, there was no evidence that the slightly higher egg output in the previously treated yearlings had an effect on the larval contamination of the second grazing season pasture. A significant yearly decrease in the second season average daily weight gains was observed, but it could not be inferred from the results of the parasitological parameters that the differences in second year growth were caused by different levels of resilience between chemoprophylactic-treated and -untreated animals. As the study covered three consecutive second grazing seasons, an effect of differences between years (e.g. in weather conditions or grass growth) on the results cannot be excluded.

Persistence of the efficacy of doramectin against Ostertagia ostertagi and Cooperia oncophora in cattle.

The persistence of the efficacy of doramectin injectable against Ostertagia ostertagi and Cooperia oncophora was evaluated in two studies in calves. In both, the calves were allocated to six groups of six. Calves in the first control group (C1) and first treated group (T1) received a daily infection of 200 L3 of O ostertagi and 200 L3 of C oncophora; the calves in groups C2 and T2 received a daily infection of 1000 L3 of each species, and groups C3 and T3 received 10,000 L3 of each species per day. The calves in the three treated groups each received 0.2 mg/kg doramectin injectable on day 0. In the first study, the calves were infected for 21 days with Cooperia and for 28 days with Ostertagia, and they were slaughtered on day 33. In the second study, the calves were infected for 21 days with both species, the infections with Cooperia and Ostertagia starting from eight and 15 days, respectively, after the treatment, and the animals were slaughtered on day 40. The calculation of the persistence of the activity of doramectin was based on its efficacy against the different developmental and adult stages of the two parasites. The data from both studies indicated that the efficacy of doramectin against Ostertagia persisted for at least five weeks, but no conclusions could be drawn about the effect of the size of the infective doses on the persistence of the activity. In contrast, the Cooperia worm counts from the second study suggested that the efficacy of doramectin against Cooperia persisted for at least four weeks when the calves were exposed to a low or moderate infection level, whereas at the highest infection level it persisted for between three and four weeks.

Persistence of the efficacy of a moxidectin gel on the establishment of cyathostominae in horses.

A controlled trial was carried out to evaluate the persistence of the effect against small strongyles of a 2 per cent moxidectin gel administered orally to foals at a dose rate of 0.4 mg/kg bodyweight. Five of nine helminth-free foals were treated on day 0, and the remaining four foals were used as controls. On days 0, 7, 14, 21 and 28 all nine foals were infected experimentally with 200,000 small strongyle larvae. Three treated and two control foals were necropsied on day 42 and the rest on day 43. Fourteen species of small or medium sized small strongyles were found in the control foals, 10 of them in all four. The mean rate of establishment of the infection was 27 per cent in the control foals and 7 per cent in the treated foals. The numbers of mucosal inhibited early L3 and late L3 were about equal in each group, but there were more than 10 times as many mucosal L4 in the control foals. No lumenal stages were recovered from the treated foals, whereas almost 50 per cent of the total burdens of the control foals consisted of these stages. The establishment of the infection was prevented by moxidectin treatment for at least two and possibly three weeks.

Effects of preventive anthelmintic treatment on acquired resistance to gastrointestinal nematodes in naturally infected cattle.

The objective of this study was to investigate the influence of different types of chemoprophylaxis in first season grazing calves on their resistance against a natural reinfection with Ostertagia ostertagi and Cooperia oncophora in the second grazing season. Thirty helminth-naive crossbred calves were randomly divided in three groups of 10 animals. The animals of group B received an ivermectin sustained release bolus on day 0. The calves of group D were treated on days 0 and 56 with a subcutaneous injection of doramectin (0.2 mg kg(-1) BW). Group C was the untreated control group ('immune' controls). Although exposure to gastrointestinal nematodes in the first grazing season was only limited, the chemoprophylactic treatments in groups B and D resulted in three distinctly different infection levels (group C > group D > group B). At the start of the second grazing season, six helminth-naive steers (group N, 'susceptible' controls) were turned out together with the second season animals. After 3 weeks of grazing, the 'susceptible' controls were slaughtered, together with four animals from each other group. Parasitological and immunological parameters indicated that resistance to reinfection with Ostertagia was reduced in the chemoprophylactic treated animals, and was negatively related to the degree of suppression of host-parasite contact in the first grazing season (group C > group D > group B > group N). None of the groups had developed a complete resistance against Cooperia yet. A negative relationship was observed between reduction of first grazing season exposure, and weight gains early in the second grazing season. The remaining animals stayed on pasture until the beginning of November. At the end of the second grazing season, levels of acquired resistance against Ostertagia infection were similar in all groups, and all animals had become immune against Cooperia. No effect of first year chemoprophylaxis on total weight gains could be demonstrated. Because of discrepancy between pasture larval counts and tracer worm counts, it was not possible to draw firm conclusions on the effect of chemoprophylaxis on pasture infestation levels in the second year.

The effect of different infection levels on acquired resistance to gastrointestinal nematodes in artificially infected cattle.

The objective of this study was to evaluate the effect of different infection levels of Ostertagia ostertagi and Cooperia oncophora in a simulated 'first grazing season' on the resistance of calves to an artificial challenge infection. The infection levels were determined by the infection schedules and the chemoprophylaxis used. Thirty six 7-11-month old Holstein-Friesian bull calves were randomly divided into four groups. The animals of group B received an ivermectin sustained release bolus (ISRB) on day 0. The calves of group D were treated on days 0 and 56 with a subcutaneous injection of doramectin (0.2 mg kg(-1) BW). Group C was the untreated control group. The calves of group N were used as helminth-naive controls, while the animals of groups B, C and D were trickle infected for 24 weeks. The infection schedules were designed to simulate the expected infection pattern for each treatment group under set-stocked conditions in temperate climate areas. After the last infection, all animals were treated with oxfendazole. One week later, all animals received a challenge infection of 50,000 O. ostertagi L3 and 100,000 C. oncophora L3, spread over 10 consecutive days. During the primary infection period the faecal egg output and the serum pepsinogen and antibody levels reflected the different levels of host-parasite contact between the groups (group C > group D > group B > group N). After the challenge infection, faecal egg counts, total Ostertagia burden, size of the adult worms and abomasal globule leucocyte counts all indicated a positive relationship between the level of Ostertagia infection during the primary infection period and the level of acquired resistance. A reduction of host-parasite contact during the primary infection period, as a consequence of the infection schedule and the chemoprophylaxis used, resulted in a diminished level of resistance to the artificial challenge infection with O. ostertagi. Faecal cultures and small intestine worm counts indicated that all previously infected groups had acquired a high degree of resistance to the Cooperia challenge infection.

Comparative studies of ivermectin and moxidectin in the control of naturally acquired cyathostome infections in horses.

The control of naturally acquired cyathostome infections in horses by treatments with ivermectin and moxidectin was evaluated in three field studies. In a first study the efficacy of both drugs was assessed in a faecal egg count reduction test. Both ivermectin and moxidectin demonstrated efficacies greater than 99 per cent for up to 60 days after treatment. In a second study, the period required for strongyle eggs to reappear was estimated in horses treated either with ivermectin or moxidectin. For the horses treated with ivermectin the period varied between 10 and approximately 13 weeks, and for moxidectin between 22 and approximately 24 weeks. With both drugs strongyle eggs started to reappear in the faeces significantly earlier in foals and young horses than in adults. In a third study, two prophylactic dosing schemes involving three ivermectin treatments at intervals of eight weeks, and two moxidectin treatments 12 weeks apart, were found to be highly effective in controlling strongyle infections of horses on pasture.

Persistence of the efficacy of pour-on and injectable moxidectin against Ostertagia ostertagi and Dictyocaulus viviparus in experimentally infected cattle.

The persistence of the efficacy of moxidectin 0.5 per cent pour-on and moxidectin 1 per cent injectable against Ostertagia ostertagi and Dictyocaulus viviparus in calves was studied in two experimental trials. In the first trial two groups of seven calves were treated with either the pour-on or the injectable formulation, while a third group remained untreated. All the animals were infected daily from Monday to Friday with infective stages of O ostertagi and D viviparus between the day of treatment (day 0) and day 33, and were necropsied for worm counts three days later. The experimental design of the second trial was similar to that of the first but the period of infection was from 28 to 45 days after the treatment, and the necropsy was five days after the last infection. In both trials both moxidectin formulations had very high efficacies (99.6 per cent) against adult and developing stages of O ostertagi and D viviparus. The higher efficacy of the moxidectin pour-on preparation against early fourth stage larvae in both trials suggested that its effect was more persistent. It was calculated that the efficacy of moxidectin against O ostertagi persisted for at least five weeks for the injectable formulation and six weeks for the pour-on. The efficacy of moxidectin against D viviparus lasted for at least six weeks for both formulations.

Evaluation of the effect of 3 probiotics on experimental Escherichia coli enterotoxaemia in weaned piglets.

In this report the efficacy of 3 different probiotics (Bacillus cereus "toyoi", Lactobacillus spp. and Streptococcus faecium) was investigated. They were supplemented in the food of recently weaned piglets that were orally infected with E. coli O141 K85ab. Supplementation could not prevent mortality and clinical symptoms nor reduce the faecal excretion of hemolytic E. coli. The possible explanations for the unsatisfying results are various.