An evaluation of dental paste-like bulk-fill composite wear using intra-oral scanner.

This study evaluates the wear resistance of dental paste-like bulk-fill composites compared to conventional paste-like composite resins using an intraoral scanner and 3-D analyzing software. Six different dental composite materials, including five bulk-fill composites and one conventional composite, were tested alongside natural human enamel as a control group. A computer-controlled chewing simulator for wear testing. A one-way ANOVA test was used to identify any significant differences between the means of the tested dental composite materials α=0.05. The results showed variability among bulk-fill composites, with some demonstrating wear resistance similar to conventional composites (p<0.05). Human enamel displayed the lowest wear values, but some bulk-fill composites matched this resistance(p>0.05). Significant variability was observed among bulk-fill composites but the results were comparable to those of conventional composites. The enamel control group demonstrated the lowest wear values, with some bulk-fill composites showing similar wear resistance. This study provides valuable information about the wear resistance of contemporary bulk-fill composite materials, commonly used in current clinical practice, contributing to enhancing clinical procedures.

The Effect of Gravity on Marginal Integrity of Different Flowable Bulk-Fill Resin Composites.

Background and Objectives: The aim of this quantitative research was to investigate the effect of gravitational forces on the marginal integrity of different bulk-fill composites by micro-CT imaging. Materials and Methods: Fifty caries-free human third molars extracted for prophylactic purposes were used in this study. Each tooth was prepared with two proximal box cavities, with dimensions of 3 mm × 3 mm × 5 mm. Five distinct groups, each comprising 20 cavities, thus totaling 100 cavities for this study: (1, Group CON): Clearfil Majesty Flow + Clearfil Majesty Esthetic (as the control); (2, Group FBR): Filtek Bulk-fill Flowable Restorative + Clearfil Majesty Esthetic; (3, Group XTB): Voco Extrabase + Clearfil Majesty Esthetic; (4, Group SDR): SDR + Clearfil Majesty Esthetic; and (5, Group SNC): Sonicfill. When restoring the mesial cavities, the occlusal surfaces of the teeth in the mold were positioned upwards, counteracting the force of gravity. In contrast, for the restoration of the distal cavities, the occlusal surfaces were aligned downwards, to be parallel with the gravitational pull. After restorative procedures, each tooth was treated with 5000 thermal cycles. A solution of ammoniacal silver nitrate (AgNO3) was employed as a tracing agent. The micro-CT scans were conducted and the total volume of silver nitrate and the total volume of restorations within the relevant region of interest were calculated in "mm3" with software. Two-way ANOVA and Tukey tests were performed at a significance level of p = 0.05 with Graphpad Prism v 8.2.1 software. Results: Both gravity effect and interaction showed no statistical differences (p > 0.05). Statistically significant differences were observed in the restorative materials (p < 0.05). Conclusions: Gravitational forces do not emerge as a major factor affecting the marginal integrity of flowable bulk-fill composites in class II restorations. The chemical composition of the composites plays a more crucial role, with the XTB composite showing higher microleakage ratios compared to the others.

Marginal adaptation of bulk-fill resin composites with different viscosities in class II restorations: a micro-CT evaluation.

BACKGROUND: The purpose of this study is to evaluate the marginal adaptation of bulk-fill resin composites with different viscosities (paste-like and flowable) in Class II restorations using micro-CT imaging. METHODS: Forty extracted human molars were used. Mesial and distal Class II box cavities (approximately 3 mm x 3 mm x 4 mm) were prepared for each tooth, with cavity floors located 1 mm below the enamel-cementum junction. Following adhesive application, teeth were restored using eight different groups: Group XB: X-tra Base Bulk-fill Flowable (VOCO), Group XF: X-tra Fill Bulk-fill (VOCO), Group FB: Filtek Bulk-fill Posterior (3 M ESPE), Group FF: Filtek Bulk-fill Flowable (3 M ESPE), Group BB: Beautifil-Bulk (SHOFU), Group BF: Beautifil-Bulk Flowable (SHOFU), and Group CO: "as a control group", Clearfil Majesty Posterior (KURARAY) and Group CF: "as a control group", Clearfil Majesty Flow + Clearfil Majesty Posterior (KURARAY). The restored teeth underwent an aging protocol involving 1000 cycles in a water bath fluctuating between 5 ± 1.0 °C and 55 ± 1.0 °C. Post-aging, teeth were immersed in 50% silver nitrate solution for 24 h and then in a film developer solution for 8 h. Microleakage analysis was performed using micro-CT, evaluated with 3D Slicer software. A two-way ANOVA was employed for statistical analysis. RESULTS: Two-way ANOVA results indicated significant effects of both viscosity (p < 0.0001) and composite type (p < 0.0001) on marginal adaptation. Viscosity analysis (comparing flowable and paste-like) revealed no significant differences in the FB-FF, XB-XF and BB-BF groups but significant differences in the and CO-CF group, with flowable type exhibiting less microleakage than paste-like type. CONCLUSIONS: The study suggests that while the viscosity of bulk-fill composites did not significantly affect marginal adaptation, the brand of bulk-fill composite did influence it.

Radiopacity of resin-based CAD/CAM blocks assessed by areal grayscale pixel value measurement.

PURPOSE: This study assessed radiopacity of resin-based computer-aided-design/computer-aided-manufacturing (CAD/CAM) materials by areal grayscale pixel value measurement. METHODS: Radiopacities of six resin-based CAD/CAM block materials and resin composite were evaluated and compared to that of enamel and dentin. Specimens of 1-mm thickness were placed on photostimulable phosphor plate and irradiated with digital x-ray unit. On the radiographic image, regions of interests were determined for each specimen and areal grayscale pixel values were measured. Elemental analysis was performed with energy-dispersive x-ray spectroscopy (EDS) on field emission scanning electron microscope (FESEM) images of the specimens. Data were analyzed statistically (α = 0.05). RESULTS: Radiopacity values of the restorative materials were significantly different from each other (P < 0.05). Radiopacity values of two resin-based CAD/CAM materials were significantly lower than that of dentin (P < 0.05). All tested restorative materials contain zirconium, three materials contain barium, and only resin composite contains lanthanum. CONCLUSION: Four CAD/CAM materials with higher amounts of zirconia or barium (>18%) had radiopacity values significantly higher than the dentin; while two materials with lower amounts of zirconia (<4%) and/or no-barium, had radiopacity values significantly lower than the dentin. EDS analysis suggests materials containing elements with higher atom numbers such as zirconia and barium could exhibit higher radiopacity.

Micro-computed tomographic evaluation of the effects of pre-heating and sonic delivery on the internal void formation of bulk-fill composites.

The aim of this study was to compare the effects of conventional, sonic or pre-heating insertion techniques on internal void formation of bulk-fill composites with micro-computed tomography. Standardized cylindrical cavities were prepared in 160 human third molars. Four groups received different paste-like bulk-fill composites: SonicFill 2 (SF2); VisCalor Bulk (VCB); Filtek One Bulk-fill restorative (FBF); Tetric EvoCeram Bulk Fill (TEB); and a conventional posterior composite, Clearfil Majesty Posterior (CMP). A hybrid CAD/CAM block was selected as a control (n=10). Composite restorations were built according to each resin composite type and insertion technique (n=10). Micro-CT was used to assess internal void rates. Data was analyzed with two-way ANOVA and Tukey's multiple comparisons test (α=0.05). CAD/CAM blocks were free of voids. For each composite, the highest void rates were observed for the sonic delivery method (p<0.05) except for SF2. SF2 was not affected by insertion techniques (p>0.05). Other composites showed the lowest void rates with pre-heating technique.

Cytotoxicity evaluation of eluates from universal adhesives by real-time cell analysis.

The aim of this study was to evaluate the cytotoxicity of universal adhesives on L929 mouse fibroblast cell line by using a real-time cell analysis. In order to obtain extract, six different cured dental adhesives were immersed in Dulbecco's Modified Eagle's Medium (DMEM) at 37°C for 24 h. A real-time cell analysis system was used to assess cytotoxicity of the dental adhesives. After seeding 25,000 cells/300 µL/well cell suspensions into the wells of an e-plate, fibroblasts were exposed to extracts of tested adhesives at varying dilutions (1:1, 1:2, and 1:10) and observed at every 30 min intervals for 72 h. Three-way ANOVA one factor repeated measures were used to analyze the results (α=0.05). All tested adhesives induced cell viability loss, cell morphology alteration, and cell death depending on extract concentration and time. Cell viability of L929 cells to between 44 and 10% for 1:1 diluted extracts, at 72 h, when compared to the negative control.

Cytotoxic effects of contemporary bulk-fill dental composites: A real-time cell analysis.

The aim of this study was to evaluate the cytotoxicity of contemporary flowable and paste-like bulk-fill dental composites by using a real-time cell analysis. In the present paper, cytotoxicity levels of five flowable, five paste-like bulk-fill composite materials and one conventional flowable, one conventional paste-like resin composite were examined on L929 mouse fibroblast cell line. After seeding 25,000 cells/300 µL/well cell suspensions into the wells of an E-plate, test materials were added and observed at every 30 min intervals for 72 h. Kruskal Wallis H and Mann Whitney U multiple comparison tests were used to analyze the results. Pre-reacted glass-ionomer (PRG) containing bulk-fill composites were severely toxic at all time points (24, 48 and 72 h, p<0.05). None of the tested composites demonstrated high cell viability (>70%) at 48 and 72 h. Flowable and paste-like composites of the same brand exhibited similar cytotoxic properties (p>0.05).

Influence of different universal adhesives on the repair performance of hybrid CAD-CAM materials.

OBJECTIVES: The aim of this study was to investigate the microshear bond strength (µSBS) of different universal adhesive systems applied to hybrid computer-aided design/computer-aided manufacturing (CAD-CAM) restorative materials repaired with a composite resin. MATERIALS AND METHODS: Four types of CAD-CAM hybrid block materials-Lava Ultimate (LA), Vita Enamic (VE), CeraSmart (CS), and Shofu Block HC (SH)-were used in this study, in combination with the following four adhesive protocols: 1) control: porcelain primer + total etch adhesive (CO), 2) Single Bond Universal (SB), 3) All Bond Universal (AB), and 4) Clearfil Universal Bond (CU). The µSBS of the composite resin (Clearfil Majesty Esthetic) was measured and the data were analyzed using two-way analysis of variance and the Tukey test, with the level of significance set at p < 0.05. RESULTS: The CAD-CAM block type and block-adhesive combination had significant effects on the bond strength values (p < 0.05). Significant differences were found between the following pairs of groups: VE/CO and VE/AB, CS/CO and CS/AB, VE/CU and CS/CU, and VE/AB and CS/AB (p < 0.05). CONCLUSIONS: The µSBS values were affected by hybrid block type. All tested universal adhesive treatments can be used as an alternative to the control treatment for repair, except the AB system on VE blocks (the VE/AB group). The µSBS values showed variation across different adhesive treatments on different hybrid CAD-CAM block types.

In vitro antibacterial activity of self-etch bio-active dental adhesives after artificial aging.

PURPOSE: The aims to evaluate the antibacterial effect of different bioactive component containing dental adhesives before and after artificial aging. MATERIALS AND METHODS: Two bio-active adhesives; Clearfil Protect Bond and FL Bond II, two non-bioactive adhesives, Clearfil SE Bond and Clearfil S3 Bond were used for this study. Antibacterial activities of the fresh and aged samples against Streptococcus mutans were investigated with Direct Contact Test. Data were analyzed with Kruskal Wallis and Mann Whitney U multiple comparison tests. RESULTS: For fresh samples FL Bond II and Clearfil Protect Bond exhibit similar antibacterial effect but Clearfil Protect Bond showed significantly higher antibacterial effect after aging the samples (p<0.05). CONCLUSION: The incorporation of bio-active antibacterial components into adhesive systems may be considered as a fundamental component in inhibiting residual Streptococcus mutans when considering the antibacterial effect of fresh samples of bio-active adhesives.

In-vitro analysis of maxillary first molars morphology using three dimensional Micro-CT imaging: considerations for restorative dentistry.

PURPOSE: The aim of this study was to determine the differences between the positional relationship of the crown contour and the pulp chamber of left and right maxillary first molars, as well as their morphological characteristics by using micro-CT system with reconstruction from a volumetric rendering software. MATERIALS AND METHODS: In total, 21 extracted maxillary first molars, including 11 left and 10 right teeth, were used. The positional relationship between the crown contour, pulp chamber and morphology of the teeth were investigated three-dimensionally by means of micro-CT imaging. RESULTS: Closest distance of mesio-buccal pulp horn to enamel surface in mm was calculated as 2.5±0.20 mm for right and 2.29±0.17 mm for left teeth. This difference was statistically significant (p=0.017). The means of closest distance of disto-buccal pulp horn to enamel surface were also significantly different between left and right teeth (p=0.001). The mean pulp volumes of right side and left side teeth were, respectively, 32.94±3.19 mm3 and 33.71±2.82 mm3 but this difference was not statistically significant. CONCLUSION: These results suggest that right and left maxillary first molars should be treated differently during preparation of cavities. Further studies must be done with larger samples as well as for other molar teeth in different populations to reveal the morphology of the molar for further considerations in restorative dentistry.

Multidisciplinary approach for the treatment of horizontal root-fractured maxillary anterior teeth.

Dental trauma can lead to a wide range of injuries of which crown and root fractures are examples. Crown-root fractures often need complex treatment planning. This case report describes the use of MTA in the multidisciplinary management of a patient with a horizontally fractured central incisor and luxation in a different central incisor. A 42-year-old female patient presented within 1 h of receiving direct trauma to her maxillary area. Clinical examination revealed that the right and left maxillary central incisors presented mobility and sensitivity to percussion and palpation but no sensitivity to thermal stimulations. Occlusal displacement with extrusion in the left maxillary central incisor and luxation in the right maxillary central incisor was observed. Radiographic examination revealed horizontal root fracture at the apical third of the left maxillary central incisor. Root fracture in the right maxillary incisor was not observed. Endodontic and aesthetic restorative treatments were completed. MTA showed a good long-term outcome when used in root-fractured and luxated teeth. In addition, composite resin restoration provided satisfactory aesthetic results even after 15 months.