1.
Mol Gen Mikrobiol Virusol
; (4): 26-32, 1987 Apr.
Article
in Russian
| MEDLINE
| ID: mdl-3037359
ABSTRACT
The structure of the cholera toxin operon and the location of A and B toxin subunits have been studied by the Southern blot hybridization on filters. The gene coding for the synthesis of the cholera toxin B-subunit has been cloned in the vector plasmid pBR322. The structural gene of A-subunit has been partially deleted by the restriction endonuclease Bal31 digestion. The size of the 250 b. p. deletion has been defined by electron microscopy. The production of the cholera toxin B-subunit in Escherichia coli K12 cells has been studied.
Subject(s)
Cholera Toxin/genetics , Cloning, Molecular , Genes, Bacterial , Peptide Fragments/genetics , Plasmids , Cholera Toxin/biosynthesis , Cholera Toxin/immunology , Cholera Vaccines/immunology , DNA Restriction Enzymes , Escherichia coli/genetics , Peptide Fragments/biosynthesis , Peptide Fragments/immunology , Transformation, Bacterial , Vibrio cholerae/genetics , Vibrio cholerae/pathogenicity
2.
Mol Gen Mikrobiol Virusol
; (2): 11-9, 1986 Feb.
Article
in Russian
| MEDLINE
| ID: mdl-3023983