ABSTRACT
A new direct radioimmunoassay measuring ovarian steroid metabolites in small aliquots of urine has been evaluated. For this purpose urinary levels of estrone-3-glucuronide (E1G) and pregnanediol-3-glucuronide (PG) were correlated with plasma values of estradiol (E2) and progesterone (P), respectively, from days 5 to 24 of the cycle in 24 women with normal cycles. Urine samples were representative of 1) first morning voiding; 2) 24-hour collection; and 3) all voidings except for the first morning one. The correlation between urinary E1G and PG with plasma values of E2 and P was statistically significant for the 3 collections of urine. The first morning voiding is selected as the sample of choice to determine E1G and PG due to its ease of collection and its high correlation with plasma E2 and P levels (r = .60, P less than .001; r = .73, P less than .001, respectively).
Subject(s)
Circadian Rhythm , Estrogens/urine , Ovarian Function Tests/methods , Radioimmunoassay , Estradiol/blood , Estrone/analogs & derivatives , Estrone/urine , Female , Glucuronates/urine , Humans , Luteinizing Hormone/blood , Menstruation , Ovulation Detection , Pregnanediol/analogs & derivatives , Pregnanediol/urine , Progesterone/bloodSubject(s)
Estradiol/blood , Menstruation , Ovary/physiology , Progesterone/blood , Animals , Female , Haplorhini , KineticsABSTRACT
Previous work in our laboratory has demonstrated the specific synthesis of a soluble protein (A) in the rat endometrium on day L4 of pseudopregnancy 1 h after the decidual induction; the A protein synthesis being higher in the antimesometrial region (AMR) than in the mesometrial one. We have now examined in the AMR of rat endometria on day L4 of pseudopregnancy: 1) the effect of cycloheximide (500 microgram, i.p. 30 min before decidual induction) on the biosynthesis of the A protein, and 2) the effect of actinomycin D (Act. D) (5 microgram/uterine horn 60 min before the decidual stimulation) on the induction of A protein synthesis. The synthesis of the A protein was determined by acrylamide gel electrophoresis of endometrial soluble proteins after double-labeling incorporation of amino acids. Results showed that: 1) the cycloheximide inhibited the synthesis of the A protein which indicates a de novo protein synthesis; 2) the Act. D inhibited the A synthesis suggesting that synthesis of an Act. D-sensitive product is one of the earliest macromolecular synthetic events after decidual induction.
