GC-MS characterization of n-hexane soluble fraction from dandelion (Taraxacum officinale Weber ex F.H. Wigg.) aerial parts and its antioxidant and antimicrobial properties.

A comparative investigation of n-hexane soluble compounds from aerial parts of dandelion (Taraxacum officinale Weber ex F.H. Wigg.) collected during different vegetative stages was carried out. The GC-MS analysis of the n-hexane (unpolar) fraction showed the presence of 30 biologically active compounds. Phytol [14.7% of total ion current (TIC)], lupeol (14.5% of TIC), taraxasteryl acetate (11.4% of TIC), ß-sitosterol (10.3% of TIC), α-amyrin (9.0% of TIC), ß-amyrin (8.3% of TIC), and cycloartenol acetate (5.8% of TIC) were identified as the major components in n-hexane fraction. The unpolar fraction exhibited promising antioxidant activity - 46.7 mmol Trolox equivalents/g extract (determined by 1,1-diphenyl-2-picrylhydrazyl method). This fraction demonstrated insignificant antimicrobial activity and can be used in cosmetic and pharmaceutical industries.

Ultrasound and Microwave-Assisted Extraction of Elecampane (Inula helenium) Roots.

The aim of the current research was to perform two "green chemistry" extractions (ultrasound and microwave irradiation) with methanol, 70% (v/v) ethanol and water for extraction of biologically active substances from elecampane (Inula heleniun L.) roots and to compare their contents in all extracts. The presence of carbohydrates (sugars, fructooligosaccharides and inulin), total phenols and flavonoids were established. In vitro antioxidant potential was also evaluated by four assays (DPPH, ABTS, FRAP and CUPRAC). Water extracts obtained by ultrasound-assisted extraction (UAE) showed the highest value of inulin (38 g/100 g dry weight plant material). The highest antioxidant activity was possessed by the 70% (v/v) ethanol extracts obtained by UAE: DPPH - 107.2 mM TE/g dw, ABTS - 86.0 mM TE/g dw, FRAP - 67.0 mM TE/g dw and CUPRAC -173.0 mM TE/g dw, respectively. The reason for this probably depended on the highest content of total phenols in the 70% UAE ethanol extract (7.9 mg GAE/g dw, phenolic acids (chlorogenic, caffeic, p-coumaric, sinapic and ferulic acids)}, especially chlorogenic acid (1.84 mg/g) and flavonoids (quercetin, kaempferol and catechin; 26.4 mg QE/g dw). UAE was evaluated as a promising approach for the simultaneous extraction of bioactive compounds (dietary fibers and antioxidants) from elecampane roots in comparison with microwave irradiation.

Protopine production by fumaria cell suspension cultures: effect of light.

Protopine biosynthesis in Fumaria rostellata and Fumaria officinalis cell suspensions was investigated. For the first time, we reported for calli and cell suspensions obtained from F. rostellata and F. officinalis. Callus induction was initiated on a Murashige and Skoog medium, supplemented with sucrose and various concentrations of plant growth regulators: 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP). The best morphological characteristics, growth behavior, and protopine biosynthesis were observed for two callus lines (5FRL14 and 12FOL1) cultivated under submerged conditions, at low concentration of 2,4-D (0.2 and 0.5 mg/L) and higher concentration of BAP (2.0 and 3.0 mg/L). The maximal yield of protopine was accumulated from cell suspension of F. rostellata (line 5FRL14) cultivated under illumination-49.6 mg/L. Time courses of utilization of sucrose, ammonium, nitrate, and phosphate ions in cultural liquid and acetylcholinesterase inhibitory activity of alkaloid extracts of studied suspensions are also presented.