Genome-resolved metagenomics reveals the nitrifiers enrichment and species succession in activated sludge under extremely low dissolved oxygen.

Nitrification, a process carried out by aerobic microorganisms that oxidizes ammonia to nitrate via nitrite, is an indispensable step in wastewater nitrogen removal. To facilitate energy and carbon savings, applying low dissolved oxygen (DO) is suggested to shortcut the conventional biological nitrogen removal pathway, however, the impact of low DO on nitrifying communities within activated sludge is not fully understood. This study used genome-resolved metagenomics to compare nitrifying communities under extremely low- and high-DO. Two bioreactors were parallelly operated to perform nitrification and DO was respectively provided by limited gas-liquid mass transfer from the atmosphere (AN reactor, DO < 0.1 mg/L) and by sufficient aeration (AE reactor, DO > 5.0 mg/L). Low DO was thought to limit nitrifiers growth; however, we demonstrated that complete nitrification could still be achieved under the extremely low-DO conditions, but with no nitrite accumulation observed. Kinetic analysis showed that after long-term exposure to low DO, nitrifiers had a higher oxygen affinity constant and could maintain a relatively high nitrification rate, particularly at low levels of DO (<0.2 mg/L). Community-level gene analysis indicated that low DO promoted enrichment of nitrifiers (the genera Nitrosomonas and Nitrospira, increased by 2.3- to 4.3-fold), and also harbored with 2.3 to 5.3 times higher of nitrification functional genes. Moreover, 46 high-quality (>90 % completeness and <5 % contamination) with 3 most abundant medium-quality metagenome-assembled genomes (MAGs) were retrieved using binning methods. Genome-level phylogenetic analysis revealed the species succession within nitrifying populations. Surprisingly, compared to DO-rich conditions, low-DO conditions were found to efficiently suppressed the ordinary heterotrophic microorganisms (e.g., the families Anaerolineales, Phycisphaerales, and Chitinophagales), but selected for the specific candidate denitrifiers (within phylum Bacteroidota). This study provides new microbial insights to demonstrate that low-DO favors the enrichment of autotrophic nitrifiers over heterotrophs with species-level successions, which would facilitate the optimization of energy and carbon management in wastewater treatment.

Insights on pretreatment technologies for partial nitrification/anammox processes: A critical review and future perspectives.

For almost 20 years, partial nitrification-anammox (PN/A) has been the subject of intensive study and development. Pretreatment of wastewater for PN/A is crucial because the inhibitory substances in the influent may reduce the performance of PN/A. In this review, the current PN/A pretreatment technologies are comprehensively summarized. The selection of pretreatment technology for PN/A depending on the source of the wastewater and its main characteristics (high-strength wastewater or municipal wastewater, organic matters, suspended solids). Comparison of pretreatment technologies through multiple perspectives including wastewater characteristics, the objectives of the wastewater treatment (treating requirement, energy and resource recovery demand), reactor configuration of PN/A. Based on the discussion, two integrated processes, HRAS + one-stage PN/A and advanced AD + two-stage PN/A, are recommended as the preferred processes for treating municipal wastewater and wastewater with a high-strength ammonium, respectively. This review aims to provide guidance for future research and development of PN/A.

Energy-neutral municipal wastewater treatment based on partial denitrification-anammox driven by side-stream sulphide.

"Low-carbon" has become an important evaluation index of modernisation construction. In the area of wastewater treatment has also caused considerable concern. Anaerobic ammonium oxidation (anammox) is a novel autotrophic nitrogen removal process that provides an opportunity for low-carbon remodelling of municipal wastewater treatment plants (MWTPs). The stable supply of nitrite is of great significance for the application of anammox. As a process with stable nitrite supply, partial denitrification (PD) is of great significance in the coupling nitrogen removal with anammox in municipal wastewater. Furthermore, innovation of the low-carbon nitrogen removal process can enable the recovery of abundant bioenergy resource from MWTPs. The low-carbon nitrogen removal via PD-anammox process and the bioenergy recovery for municipal wastewater in the previous studies has been summarised. On this basis, a novel energy-neutralisation municipal wastewater treatment process based on partial denitrification-anammox driven by sulphide produced in the side-stream has been proposed. The long-term retention of mainstream anammox and improvement of energy recovery efficiency under the requirement of ensuring nitrogen removal require additional detailed investigation.

Low-carbon nitrogen removal from power plants circulating cooling water and municipal wastewater by partial denitrification-anammox.

As a reclaimed water reuse strategy, using treated municipal wastewater as power plants circulating cooling water (PPCCW) generates nitrate-rich wastewater due to evaporation requiring retreatment. An innovative low-carbon nitrogen removal process, partial denitrification-anammox (PD-A), was used in this study. The PPCCW and municipal wastewater pre-treated with 10 mg/L Fe3+ were simultaneously subjected to the PD-A process. The results showed that the total nitrogen of effluent less than 10 mg/L, and a removal efficiency of 79.67 ± 3.48% was attained. Unclassified_f_Brocadiaceae was the dominant anammox genus, with an increasing percentage (from 0.42 to 1.27%), laterally indicating the reactor stability. Furthermore, the hydrolytic acidifying bacteria SBR1031 and Bacillus increased substantially after feeding with actual wastewater, and the removal efficiencies of organic material and nitrogen increased, indicating that hydrolytic acidifying bacteria have a synergistic effect with PD-A bacteria. Finally, a novel wastewater treatment process that fully recovers carbon, phosphorus, and water was proposed.