ABSTRACT
AIM: To prepare specific antibodies against ciprofloxacin (CIP) and characterize the antibodies properities with ELISA, then lay the foundation for development of CIP rapid detection kit. METHODS: New Zealand white rabbits and BALB/c mice were immunized with CIP-cBSA conjugate, CIP-nOVA and CIP-cOVA serving as coating antigen were used to screen antisera, indirect (competitive) ELISA was developed to determine the titer and specificity of the antisera. RESULTS: Seven antisera against CIP were obtained, two of them were high specific to CIP with IC(50); 1 µg/L, the titer of which was 4 000 and 2 000, respectively.The antisera had some degree cross-reactivity with ENR, NOR and OFL, but had hardly cross-reactivity with PEN, KAN and QEN. CONCLUSION: The antibodies with high specificity developed in this study can be used to develop CIP detecting kit.
