ABSTRACT
AIM: To explore the expression of albumin (ALB), insulin-like growth factor (IGF)-1, and insulin-like growth factor binding protein (IGFBP)-3 in tumor tissues and adjacent non-tumor tissues of hepatocellular carcinoma (HCC) patients with cirrhosis. METHODS: Twenty-four HCC patients with cirrhosis who underwent hepatectomy were studied. ALB mRNA, IGF-1 mRNA, and IGFBP-3 mRNA in liver tissues (including tumor tissues and adjacent non-tumor tissues) were detected by reverse transcriptase-polymerase chain reaction (RT-PCR). Liver Ki67 immunohistochemistry staining was studied. At the same time, 12 patients with cholelithiasis or liver angioma who underwent operation were segregated as normal control. RESULTS: In HCC patients with cirrhosis, hepatic ALB mRNA, IGF-1 mRNA, and IGFBP-3 mRNA of tumor tissues or adjacent non-tumor tissues were lower than the normal liver tissues, while in tumor tissues, hepatic ALB mRNA and IGFBP-3 mRNA were lower, hepatic IGF-1 mRNA was higher than in adjacent non-tumor tissues. Liver Ki67 labeling index (Ki67 LI) in tumor tissues or adjacent non-tumor tissues were higher than that in the normal liver tissues, while in tumor tissues it was higher than that in adjacent non-tumor tissues. CONCLUSION: Imbalance of IGF-1 and IGFBP-3 may play a role in hepatocarcinogenesis and tumor development of liver cirrhosis patients.
Subject(s)
Albumins/genetics , Carcinoma, Hepatocellular/physiopathology , Insulin-Like Growth Factor Binding Protein 3/genetics , Insulin-Like Growth Factor I/genetics , Liver Neoplasms/physiopathology , Carcinoma, Hepatocellular/genetics , Gene Expression Regulation, Neoplastic , Humans , Liver Cirrhosis/genetics , Liver Cirrhosis/physiopathology , Liver Neoplasms/geneticsABSTRACT
OBJECTIVE: To study the reasons and possibly solutions for missed diagnosis of duodenal injury. METHOD The clinical records were retrospectively analyzed in 9 cases of missed diagnosis of duodenal injury treated in our hospital in the year 1992 to 2002. RESULTS Of the 15 cases of duodenal injury, missed diagnoses occurred in 9 cases. The reasons for the missed diagnoses included complications by injuries of the other abdominal organs that concealed duodenal injuries, neglect of the light injuries, and the lack of clinical experience on the part of the young doctors. CONCLUSION Duodenal injury frequently occurs along with other abdominal organ injuries and is easy to escape detection, which can be avoided by careful preoperative examination of the characteristics of injuries and the patients' clinical manifestations after the injury.
