Stable isotope tracing internal recycling and evaporation losses in saline lakes on the Qinghai-Tibet Plateau.

Direct measuring of internal lake recycling and evaporation losses remains challenging for lakes on the Qinghai-Tibet Plateau (QTP). Stable isotope techniques provide an effective approach for estimating water vapor cycling ratios and evaporation losses of lakes on the QTP. In this study, the stable isotope values of saline lakes on the QTP were modeled using the stable isotope values of the sampled lake water and their influencing factors. The water vapor recycling ratio and evaporation loss (E/I) of 135 saline lakes on the QTP were evaluated and their influencing factors were revealed. The results showed that stable isotopes in saline lakes on the QTP showed significant spatial variability. Their stable isotopes were affected by the source of water vapor, recharge patterns, and local evaporation conditions. It's worth noting that the average water vapor recycling ratio of saline lakes on the QTP was 20.16 %, one-fifth of the saline lakes had a water vapor recycling ratio beyond 30 %. Saline lakes lose 26 % of their water through evaporation. 26 % of the saline lakes experienced high evaporation losses of >40 % of the total inflow. We found that the main factors controlling the water vapor recycling ratio and evaporation loss in saline lakes on the QTP were precipitation and altitude, respectively. Interestingly, the control factors of water vapor recycling ratio and evaporation loss in saline lakes with elevation above 4500 m showed significant differences compared to saline lakes with elevation below 4500 m. Therefore, the strengthening of lake system monitoring can provide reliable data support for security assessment and effective management of water resources on the QTP.

Deep Succinylproteomics of Brain Tissues from Intracerebral Hemorrhage with Inhibition of Toll-Like Receptor 4 Signaling.

It is unclear how Toll-like receptor (TLR) 4 signaling affects protein succinylation in the brain after intracerebral hemorrhage (ICH). Here, we constructed a mouse ICH model to investigate the changes in ICH-associated brain protein succinylation, following a treatment with a TLR4 antagonist, TAK242, using a high-resolution mass spectrometry-based, quantitative succinyllysine proteomics approach. We characterized the prevalence of approximately 6700 succinylation events and quantified approximately 3500 sites, highlighting 139 succinyllysine site changes in 40 pathways. Further analysis showed that TAK242 treatment induced an increase of 29 succinyllysine sites on 28 succinylated proteins and a reduction of 24 succinyllysine sites on 23 succinylated proteins in the ICH brains. TAK242 treatment induced both protein hypersuccinylations and hyposuccinylations, which were mainly located in the mitochondria and cytoplasm. GO analysis showed that TAK242 treatment-induced changes in the ICH-associated succinylated proteins were mostly located in synapses, membranes and vesicles, and enriched in many cellular functions/compartments, such as metabolism, synapse, and myelin. KEGG analysis showed that TAK242-induced hyposuccinylation was mainly linked to fatty acid metabolism, including elongation and degradation. Moreover, a combined analysis of the succinylproteomic data with previously published transcriptome data revealed that most of the differentially succinylated proteins induced by TAK242 treatment were mainly distributed throughout neurons, astrocytes, and endothelial cells, and the mRNAs of seven and three succinylated proteins were highly expressed in neurons and astrocytes, respectively. In conclusion, we revealed that several TLR4 signaling pathways affect the succinylation processes and pathways in mouse ICH brains, providing new insights on the ICH pathophysiological processes. Data are available via ProteomeXchange with identifier PXD025622.

Limitations of soil moisture and formation rate on vegetation growth in karst areas.

Vegetation changes in karst areas are controlled by the soil formation rate (SFR) and soil moisture (SM). However, little is known about their thresholds and global control patterns. To this end, based on high-precision climate and vegetation data for 2000-2014, using Pearson correlation analysis, the Hurst index, and change-point analysis, the thresholds of the SFR and SM in vegetation growth in karst areas were identified. Furthermore, a spatial map (0.125° × 0.125°) of the global karst ecosystem with a static/dynamic limitation zone was established. We found that the net primary productivity (NPP) in 70% of the global climate zones exhibited a dual restriction relationship with the SM and SFR. The limitations of the SFR and SM in vegetation growth were most obvious in subpolar and semi-arid climates. In addition, their ecological thresholds were 25.2 t km-2 yr-1 and 0.28 m3 m-3, respectively. The static limitation of the SFR on the NPP in karst areas accounted for 28.37%, and the influence of the SM enhanced this limit (21.79%). The limitation of the SFR on vegetation was mainly concentrated in Boreal forests (17%), and the limitation of the SM was mainly concentrated in tropical savannas (12%). The NPP and the Normalized Difference Vegetation Index (NDVI) were the most sensitive to changes in the SM and SFR. Moreover, the analysis based on 14 ecologically limitation karst areas further revealed that the reduction in these factors may cause the tropical rain forest to experience degradation. It can be seen that the SM enhanced the limiting effect of the SFR on vegetation in karst areas. In short, this interpretation of karst vegetation limitations provides a deeper understanding of and approach to ecosystem evolution and vegetation restoration in these regions.

Succinylation profiles of brain injury after intracerebral hemorrhage.

Protein posttranslational modifications (PTMs) regulate the biological processes of human diseases by genetic code expansion and cellular pathophysiology regulation; however, system-wide changes in PTM levels in the intracerebral hemorrhage (ICH) brain remain poorly understood. Succinylation refers to a major PTM during the regulation of multiple biological processes. In this study, according to the methods of quantitative succinyllysine proteomics based on high-resolution mass spectrometry, we investigated ICH-associated brain protein succinyllysine modifications and obtained 3,680 succinylated sites and quantified around 3,530 sites. Among them, 25 succinyllysine sites on 23 proteins were upregulated (hypersuccinylated), whereas 13 succinyllysine sites on 12 proteins were downregulated (hyposuccinylated) following ICH. The cell component enrichment analysis of these succinylproteins with significant changes showed that 58.3% of the hyposuccinylated proteins were observed in the mitochondria, while the hyper-succinylproteins located in mitochondria decreased in the percentage to about 35% in ICH brains with a concomitant increase in the percentage of cytoplasm to 30.4%. Further bioinformatic analysis showed that the succinylproteins were mostly mitochondria and synapse-related subcellular located and involved in many pathophysiological processes, like metabolism, synapse working, and ferroptosis. Moreover, the integrative analysis of our succinylproteomics data and previously published transcriptome data showed that the mRNAs matched by most differentially succinylated proteins were especially highly expressed in neurons, endothelial cells, and astrocytes. Our study uncovers some succinylation-affected processes and pathways in response to ICH brains and gives us novel insights into understanding pathophysiological processes of brain injury caused by ICH.

Global patterns and changes of carbon emissions from land use during 1992-2015.

Carbon emissions from land use (E LUC) are an important part of anthropogenic CO2 emissions, but its size and location remain uncertain, and our knowledge of the relationship between E LUC and GDP remains partial. We showed that the carbon emissions directly caused by land use change (direct E LUC) during 1992-2015 was 26.54 Pg C (1.15 Pg C yr-1), with a decreased trend and a net reduction rate of -0.15 Pg C yr-1. The areas that exhibited reductions were concentrated in South America, Central Africa, and Southeast Asia, and those with increments were scattered in Northwestern North America, Eastern South America, Central Africa, East Asia, and parts of Southeast Asia. For the indirect carbon emissions from the utilization of built-up land (indirect E LUC), it manifested an upward trend with a total emission of 27.51 Pg C (1.2 Pg C yr-1). The total value resulted by global E LUC was $136.3 × 109 US, and the value of annual was equivalent to 3.7 times the GDP of the Central African Republic in 2015 ($5.93 × 109 US yr-1). Among the 79 countries and regions considered in this study, 54 represented the upward GDP with increased emissions, and only 25 experienced GDP growth with emission reductions. These findings highlight the pivotal role of land use change in the carbon cycle and the significance of coordinated development between GDP and carbon emissions.

Ecological security and health risk assessment of soil heavy metals on a village-level scale, based on different land use types.

Land use affects the accumulation of heavy metals in soil, which will endanger ecological safety and human health. Taking the village as an administrative unit, the ecological safety and health risks of heavy metals, namely, Cr, Cu, Zn, and Pb in soils in the Houzhai River Watershed of Guizhou Province, China, were evaluated based on land use types by the Hakanson potential ecological risk methods and human health risk model. Results showed that the spatial heterogeneity of Cu and Zn was greatly affected by primary structural factors, and Cr and Pb were interfered by both structural factors and human activities. The geo-accumulation index of the heavy metals showed a light pollution in the study area. The comprehensive potential ecological risk of heavy metal in the area was divided into three levels: slight, moderate, and intense, and it is spatially high in the northwest and low in the southeast. Both non-carcinogenic risk and carcinogenic risk of the heavy metals to the human body are not significant and are acceptable. The risks of children are higher than adults, and direct intake is the primary route of exposure in the area. The potential ecological risk and human health risk of soil heavy metals are relatively obviously affected by digital elevation data and normalized vegetation index. The study has certain reference value for the prevention and control of regional soil heavy metal risk.

Relationship among land surface temperature and LUCC, NDVI in typical karst area.

Land surface temperature (LST) can reflect the land surface water-heat exchange process comprehensively, which is considerably significant to the study of environmental change. However, research about LST in karst mountain areas with complex topography is scarce. Therefore, we retrieved the LST in a karst mountain area from Landsat 8 data and explored its relationships with LUCC and NDVI. The results showed that LST of the study area was noticeably affected by altitude and underlying surface type. In summer, abnormal high-temperature zones were observed in the study area, perhaps due to karst rocky desertification. LSTs among different land use types significantly differed with the highest in construction land and the lowest in woodland. The spatial distributions of NDVI and LST exhibited opposite patterns. Under the spatial combination of different land use types, the LST-NDVI feature space showed an obtuse-angled triangle shape and showed a negative linear correlation after removing water body data. In summary, the LST can be retrieved well by the atmospheric correction model from Landsat 8 data. Moreover, the LST of the karst mountain area is controlled by altitude, underlying surface type and aspect. This study provides a reference for land use planning, ecological environment restoration in karst areas.

The protein import pore Tom40 in the microsporidian Nosema bombycis.

Microsporidia, an unusual group of unicellular parasites related to fungi, possess a highly reduced mitochondrion known as the mitosome. Since mitosomes lack an organellar genome, their proteins must be translated in the cytosol before being imported into the mitosome via translocases. We have identified a Tom40 gene (NbTom40), the main component of the translocase of the outer mitochondrial membrane, in the genome of the microsporidian Nosema bombycis. NbTom40 is reduced in size, but it is predicted to form a ß-barrel structure composed of 19 ß-strands. Phylogenetic analysis confirms that NbTom40 forms a clade with Tom40 sequences from other species, distinct from a related clade of voltage-dependent anion channels (VDACs). The NbTom40 contains a ß-signal motif that the polar residue is substituted by glycine. Furthermore, we show that expression of NbTom40, as a GFP fusion protein within yeast cells, directs GFP to mitochondria of yeast. These findings suggest that NbTom40 may serve as an import channel of the microsporidian mitosome and facilitate protein translocation into this organelle.