ABSTRACT
As a result of climate change as well as the increasing need for island ecological protection, nature-based solutions have attracted increasing international attention. The impact of climate change and COVID-19 has presented island countries and regions with practical challenges such as rising sea levels, stunted economic development and difficult survival situations. Therefore, finding ways to realize the sustainable development of islands from a nature-based perspective has become a key concern. The Island Research Center of the Ministry of Natural Resources of China etc. organized the International Forum on Island Ecological Protection 2021 on November 19, 2021 with the theme, 'Nature-based Island Ecological Restoration and Management Practice.' The conference, which was held online and face-to-face, brought together front-line staff from government agencies, academic institutions and experts from major countries around the world to discuss emerging issues of sustainable island development.
ABSTRACT
PREMISE OF THE STUDY: To examine patterns of genetic diversity and test possible hybridization events, microsatellite markers were identified and characterized in Hoya ledongensis (Apocynaceae), and cross-amplification was tested in a congener, H. jianfenglingensis. METHODS AND RESULTS: Based on the transcriptome data of H. ledongensis, 46 microsatellite primer pairs were randomly selected for initial validation. From these, 28 primer pairs were successfully amplified, 12 of which were polymorphic in 36 individuals across three populations of H. ledongensis. The number of alleles per microsatellite locus ranged from two to 11. The observed and expected heterozygosities for the 12 loci ranged from 0.133 to 0.867 and 0.128 to 0.894, respectively. Cross-species amplification was successful for these 12 loci in the congeneric species H. jianfenglingensis. CONCLUSIONS: These polymorphic transcriptome-derived simple sequence repeat markers have the potential to be used as multilocus molecular markers to study the population genetics and natural hybridization in species of Hoya.
