ABSTRACT
Diamond like carbon (DLC) coatings were deposited on to Ti substrates by plasma enhanced chemical vapor deposition technique. Ti and DLC/Ti samples were implanted in skeletal muscle of rabbits. The samples were explanted after 1, 3, 6 and 12 months and the tissue-cell interaction was studied. Our data indicate both DLC/Ti and bare Ti to be compatible with skeletal muscle.
Subject(s)
Coated Materials, Biocompatible , Diamond , Materials Testing/methods , Muscle, Skeletal/pathology , Titanium , Animals , Carbon/classification , Carbon/pharmacology , Materials Testing/standards , Muscle, Skeletal/immunology , Prostheses and Implants , Rabbits , Time FactorsABSTRACT
Measurement of platelet adhesion in vitro is a good indicator of its reactivity to implant devices in vivo. Platelets were labeled with I-125 without affecting its normal morphology and function and the labeled platelets were suspended in platelet poor plasma and exposed to Ti and diamond like carbon-coated (DLC) Ti discs, under dynamic conditions, using a parallel plate flow chamber. The test materials were washed, dried, exposed to a phosphor screen and scanned to get the images. The number of platelets that adhered to Ti was found to be higher than those that adhered to DLC coated Ti sample, irrespective of the shear stress which was varied between 2 and 16 dynes/cm(2).
Subject(s)
Blood Platelets/diagnostic imaging , Coated Materials, Biocompatible , Diamond , Materials Testing/methods , Platelet Adhesiveness , Titanium , Blood Platelets/physiology , Carbon , Cell Adhesion , Cell Count , Humans , In Vitro Techniques , Iodine Radioisotopes , Isotope Labeling/methods , Radionuclide ImagingABSTRACT
Diamond like carbon (DLC) films were deposited on to titanium (Ti) substrates by Plasma Enhanced Chemical Vapour Deposition (PECVD) process. The quality of the films were checked by Raman spectra and nano-hardness tests. The cytocompatibility of titanium and DLC coated titanium were studied using continuous cell lines of mouse fibroblast cells ( L-929), Human Osteoblast cells (HOS) and primary human umbilical cord vein endothelial cells (HUVEC). The cellular responses to the materials were assessed both quantitatively and qualitatively. The adhesion and spreading of cells on materials were compared using Ti as a control. Present study indicates an improved cytocompatibility of DLC coated Ti in comparison to bare Ti.
