ABSTRACT
The aim of this study was to assess the diagnostic value of Peroxidase Linked Assay (PLA) for detecting the bovine leukemia virus (BLV) infection. Two negative and two positive reference sera were applied to 204 serum samples from dairy cows from 3 different farms (I, II and III). The best results of serological testing were obtained in the herd infected with BLV for several years (I). Amongst 10 serum samples taken at random, all were positive in AGID, ELISA and PLA. Serological tests of samples from group II revealed: 34 (73.9%) positive results in AGID and 39 (84.7%) either in ELISA or PLA. In group III positive results in AGID occured in 81.8% of cases, and 91.9% and 92.9% in ELISA and PLA respectively. The mean OD value for positive samples in groups II and III ranged between 0.943 and 0.215. Immunoperoxidase PLA is a specific and sensitive test for detection of BLV infections.
Subject(s)
Antibodies, Viral/blood , Enzootic Bovine Leukosis/diagnosis , Enzyme-Linked Immunosorbent Assay/veterinary , Immunodiffusion/veterinary , Leukemia Virus, Bovine/immunology , Animals , Cattle , Dairying , Enzootic Bovine Leukosis/blood , Female , Leukemia Virus, Bovine/isolation & purification , Predictive Value of Tests , Sensitivity and SpecificityABSTRACT
In the years 1992 to 2002 the ID and ELISA systemic serological examinations of infected cows from a large farm brought advantageous results. A total of 468 cows from three farms from Opole province were examined. In the years 1997 to 2002 there were four times more positive serological results obtained than in the years 1992 to 1996. The elimination of BLV infected animals from farms and watering of serologically negative calves with milk substitute feed were very useful methods. In the year 2002 the control of all cattle gave negative ELISA results based on anti-gp51 monoclonal antibodies. The farm demonstrated BLV-free status after 10 years as a result of regular laboratory testing and successful elimination of all positive animals.
Subject(s)
Enzootic Bovine Leukosis/epidemiology , Enzootic Bovine Leukosis/prevention & control , Leukemia Virus, Bovine/immunology , Animal Feed , Animals , Cattle , Enzootic Bovine Leukosis/blood , Enzootic Bovine Leukosis/etiology , Enzyme-Linked Immunosorbent Assay/veterinary , Female , Immunodiffusion/veterinary , Leukemia Virus, Bovine/isolation & purification , Male , Poland/epidemiologyABSTRACT
This study demonstrates the usefulness of PLA and nested-PCR methods for the detection of cattle infection with enzootic bovine leukemia virus. Serological control with PLA was by 1.7% more sensitive than reference ELISA examination. Both the DNA of env-BLV gene in NCR, V-NCR, FLK and in recombinated AG clone cells, as well as in the whole blood and in blood isolated leukocytes of dairy cattle, were detected with the nested-PCR.
