ABSTRACT
Application of anxiolytic drug Selank to hippocampal slices increased the amplitude and discharge rate of spontaneous inhibitory postsynaptic currents in rat hippocampal pyramidal CA1 neurons. In some neurons, Selank-induced up-regulation of spontaneous inhibitory postsynaptic currents was preceded by a transient decrease in this activity. In the examined concentration range (1-8 µM), Selank demonstrated no significant dose-dependence.
Subject(s)
Neurons/physiology , Oligopeptides/pharmacology , Synaptic Transmission/drug effects , Animals , CA1 Region, Hippocampal/cytology , Drug Evaluation, Preclinical , Inhibitory Postsynaptic Potentials/drug effects , Neurons/drug effects , Rats, WistarABSTRACT
Application of nootropic agent Noopept on hippocampal slices from Wistar rats enhanced the inhibitory component of total current induced by stimulation of Shaffer collaterals in CA1 pyramidal neurons, but did not affect the excitatory component. A direct correlation between the increase in the amplitude of inhibitory current and agent concentration was found. The substance did not affect the release of inhibitory transmitters from terminals in the pyramidal neurons, which indicated changes in GABAergic interneurons.
Subject(s)
Dipeptides/pharmacology , Hippocampus/drug effects , Hippocampus/metabolism , Nootropic Agents/pharmacology , Synaptic Transmission/drug effects , Animals , Interneurons/drug effects , Interneurons/metabolism , Rats , Rats, WistarABSTRACT
A method of classifying cataractous changes in excised human lenses, based on separate and independent photographic documentation, with consequent quantitative evaluation of lens opacification is proposed. The degree of lens clouding was evaluated quantitatively by measuring the optical density index and the area of the zones of clouding with certain optical density parameters (equidensities) by use of an image analyser. This technique produced an image in which contrast represented different zones of lens clouding in the frontal projection, and allowed their contribution to the total lens area to be determined. The precise topography of the opacities was established. The classification data obtained for different clinical types of cataracts can be simplified according to the needs of the scientist in his effort to measure clinically or scientifically significant quantitative associations between laboratory measures and cataractous changes.
