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1.
Cell Mol Life Sci ; 67(24): 4213-32, 2010 Dec.
Article in English | MEDLINE | ID: mdl-20574651

ABSTRACT

ADAMTS-2 is a metalloproteinase that plays a key role in the processing of fibrillar procollagen precursors into mature collagen molecules by excising the amino-propeptide. We demonstrate that recombinant ADAMTS-2 is also able to reduce proliferation of endothelial cells, and to induce their retraction and detachment from the substrate resulting in apoptosis. Dephosphorylation of Erk1/2 and MLC largely precedes the ADAMTS-2 induced morphological alterations. In 3-D culture models, ADAMTS-2 strongly reduced branching of capillary-like structures formed by endothelial cells and their long-term maintenance and inhibited vessels formation in embryoid bodies (EB). Growth and vascularization of tumors formed in nude mice by HEK 293-EBNA cells expressing ADAMTS-2 were drastically reduced. A similar anti-tumoral activity was observed when using cells expressing recombinant deleted forms of ADAMTS-2, including catalytically inactive enzyme. Nucleolin, a nuclear protein also found to be associated with the cell membrane, was identified as a potential receptor mediating the antiangiogenic properties of ADAMTS-2.


Subject(s)
ADAM Proteins/metabolism , Angiogenesis Inhibitors/metabolism , Neoplasms/metabolism , Neovascularization, Pathologic , Procollagen N-Endopeptidase/metabolism , ADAM Proteins/genetics , ADAMTS Proteins , ADAMTS4 Protein , Animals , Apoptosis/physiology , Cattle , Cell Line , Cell Proliferation , Embryoid Bodies/metabolism , Endothelial Cells/cytology , Endothelial Cells/physiology , Extracellular Signal-Regulated MAP Kinases/metabolism , Humans , Mice , Mice, Knockout , Mice, Nude , Neoplasms/pathology , Neoplasms, Experimental , Procollagen N-Endopeptidase/genetics , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Signal Transduction/physiology
2.
J Colloid Interface Sci ; 321(1): 154-8, 2008 May 01.
Article in English | MEDLINE | ID: mdl-18295227

ABSTRACT

In this work, some of the physicochemical properties of enzymatically prepared n-alkyl esters of glucuronic acid are presented. Two questions are addressed. The first concerns the influence of post-purification freeze-drying conditions on octyl glucuronate thermotropic behavior. Depending on the amount of water added before freeze-drying, the alpha/beta anomeric ratio determined by (1)H NMR is affected and differences are observed in DSC thermograms probably due to polymorphism. The second question concerns the effect of hydrophobic chain length on the thermal behavior. An increase of both transition temperature and transition enthalpy is observed by increasing the number of carbon atoms in the alkyl chain (C8

Subject(s)
Esters/chemical synthesis , Glucuronic Acid/chemistry , Lipase/chemistry , Surface-Active Agents/chemical synthesis , Esters/chemistry , Freeze Drying , Fungal Proteins , Hydrophobic and Hydrophilic Interactions , Magnetic Resonance Spectroscopy , Surface-Active Agents/chemistry
3.
Neuropathol Appl Neurobiol ; 32(6): 575-84, 2006 Dec.
Article in English | MEDLINE | ID: mdl-17083472

ABSTRACT

Astrocytic tumours are associated with dismal prognoses due to their pronounced ability to diffusely invade the brain parenchyma. Various neuropeptides, including gastrin, are able to modulate tumour astrocyte migration. While neurotensin has been shown to influence the proliferation of glioma cells and the migratory ability of a large set of other cell types, its role in glioma cell migration has never been investigated. Neurotensin-induced modifications to the motility features of human U373 glioblastoma cells therefore constitute the topic of the present study. We evidenced that three subtypes of neurotensin receptors (NTR1, NTR2 and NTR3) are expressed in U373 glioblastoma cells, at least as far as their mRNAs are concerned. Treating U373 tumour cells with 10 nM neurotensin markedly modified the morphological patterns of these cells and also profoundly altered the organization of their actin cytoskeletons. Pull-down assays revealed that neurotensin induced the activation in U373 cells of both Rac1 and Cdc42 but not RhoA. Scratch wound assays evidenced that neurotensin (0.1 and 10 nM) very significantly inhibited wound colonization by U373 cells cultured in the absence of serum. In addition, quantitative phase-contrast videomicroscopy analyses showed that neurotensin decreases the motility levels of U373 glioblastoma cells when these cells are cultured on plastic. In sharp contrast, neurotensin stimulates the motility of U373 cells when they are cultured on laminin, which is a pro-adhesive extracellular matrix component ubiquitously secreted by glioma cells. Our data thus strongly suggest that, in addition to gastrin, neurotensin is a neuropeptide capable of modulating tumour astrocyte migration into the brain parenchyma.


Subject(s)
Brain Neoplasms/metabolism , Cell Movement/physiology , Glioblastoma/metabolism , Neoplasm Invasiveness , Neurotensin/metabolism , Actins/metabolism , Cell Line, Tumor , Cytoskeleton/metabolism , Enzyme Activation/physiology , Humans , In Vitro Techniques , Microscopy, Phase-Contrast , Microscopy, Video , RNA, Messenger/analysis , Receptors, Neurotensin/biosynthesis , cdc42 GTP-Binding Protein/metabolism , rac1 GTP-Binding Protein/metabolism , rhoA GTP-Binding Protein/metabolism
4.
FASEB J ; 16(6): 555-64, 2002 Apr.
Article in English | MEDLINE | ID: mdl-11919158

ABSTRACT

Membrane type 1 metalloprotease (MT1-MMP) is a transmembrane metalloprotease that plays a major role in the extracellular matrix remodeling, directly by degrading several of its components and indirectly by activating pro-MMP2. We investigated the effects of MT1-MMP overexpression on in vitro and in vivo properties of human breast adenocarcinoma MCF7 cells, which do not express MT1-MMP or MMP-2. MT1-MMP and MMP-2 cDNAs were either transfected alone or cotransfected. All clones overexpressing MT1-MMP 1) were able to activate endogenous or exogenous pro-MMP-2, 2) displayed an enhanced in vitro invasiveness through matrigel-coated filters independent of MMP-2 transfection, 3) induced the rapid development of highly vascularized tumors when injected subcutaneously in nude mice, and 4) promoted blood vessels sprouting in the rat aortic ring assay. These effects were observed in all clones overexpressing MT1-MMP regardless of MMP-2 expression levels, suggesting that the production of MMP-2 by tumor cells themselves does not play a critical role in these events. The angiogenic phenotype of MT1-MMP-producing cells was associated with an up-regulation of VEGF expression. These results emphasize the importance of MT1-MMP during tumor angiogenesis and open new opportunities for the development of anti-angiogenic strategies combining inhibitors of MT1-MMP and VEGF antagonists.


Subject(s)
Endothelial Growth Factors/biosynthesis , Lymphokines/biosynthesis , Mammary Neoplasms, Experimental/metabolism , Metalloendopeptidases/biosynthesis , Neovascularization, Pathologic/metabolism , Up-Regulation , Animals , Aorta/physiology , Cell Division , Cell Movement , Clone Cells , Culture Techniques , Endothelial Growth Factors/genetics , Female , Humans , Lymphokines/genetics , Mammary Neoplasms, Experimental/blood supply , Mammary Neoplasms, Experimental/genetics , Mammary Neoplasms, Experimental/pathology , Matrix Metalloproteinase 14 , Matrix Metalloproteinase 2/biosynthesis , Matrix Metalloproteinase 2/genetics , Matrix Metalloproteinases, Membrane-Associated , Metalloendopeptidases/genetics , Mice , Mice, Nude , Neoplasm Transplantation , RNA, Messenger/biosynthesis , Rats , Transcriptional Activation , Transfection , Tumor Cells, Cultured , Vascular Endothelial Growth Factor A , Vascular Endothelial Growth Factors
5.
Cancer Res ; 61(8): 3450-7, 2001 Apr 15.
Article in English | MEDLINE | ID: mdl-11309307

ABSTRACT

The tissue inhibitor of metalloproteinases-2 (TIMP-2) has at least two independent functions, i.e., regulation of matrix metalloproteinases and growth promoting activity. We investigated the effects of TIMP-2 overexpression, induced by retroviral mediated gene transfer, on the in vivo development of mammary tumors in syngeneic mice inoculated with EF43.fgf-4 cells. The EF43.fgf-4 cells established by stably infecting the normal mouse mammary EF43 cells with a retroviral expression vector for the fgf-4 oncogene, are highly tumorigenic and overproduce vascular endothelial growth factor (VEGF). Despite a promotion of the in vitro growth rate of EF43.fgf-4 cells overexpressing timp-2, the in vivo tumor growth was delayed. At day 17 post-cell injection, the volume of tumor derived from TIMP-2-overexpressing cells was reduced by 80% as compared with that obtained with control cells. Overexpression of TIMP-2 was associated with a down-regulation of VEGF expression in vitro and in vivo, a reduction of vessel size, density, and blood supply in the induced tumors. In addition, TIMP-2 completely inhibited the angiogenic activity of EF43.fgf-4 cell-conditioned medium in vitro using a rat aortic ring model. Our findings suggest that overexpression of TIMP-2 delays growth and angiogenesis of mammary carcinoma in vivo and that down-regulation of VEGF expression may play an important role in this TIMP-2-mediated antitumoral and antiangiogenic effects. Finally the in vivo delivery of TIMP-2, as assessed by i.v. injection of recombinant adenoviruses vectors, significantly reduced the growth of the EF43.fgf-4-induced tumors. This effect of TIMP-2 was shown to be equally comparable with that of angiostatin, a known potent inhibitor of angiogenesis.


Subject(s)
Endothelial Growth Factors/biosynthesis , Lymphokines/biosynthesis , Mammary Neoplasms, Experimental/blood supply , Mammary Neoplasms, Experimental/pathology , Neovascularization, Pathologic/pathology , Tissue Inhibitor of Metalloproteinase-2/physiology , Adenoviridae/genetics , Angiostatins , Animals , Cell Division , Down-Regulation , Endothelial Growth Factors/genetics , Female , Fibroblast Growth Factors/genetics , Gene Transfer Techniques , Lymphokines/genetics , Mammary Neoplasms, Experimental/metabolism , Mice , Mice, Inbred BALB C , Neovascularization, Pathologic/genetics , Neovascularization, Pathologic/metabolism , Peptide Fragments/genetics , Peptide Fragments/physiology , Plasminogen/genetics , Plasminogen/physiology , Rats , Tissue Inhibitor of Metalloproteinase-2/biosynthesis , Tissue Inhibitor of Metalloproteinase-2/genetics , Tumor Cells, Cultured , Vascular Endothelial Growth Factor A , Vascular Endothelial Growth Factors
6.
Cardiovasc Res ; 49(3): 647-58, 2001 Feb 16.
Article in English | MEDLINE | ID: mdl-11166278

ABSTRACT

OBJECTIVE: This investigation aimed at determining the importance of the rigidity of the adhesive support and the participation of the cytoskeleton in tubulogenesis of endothelial cells in vitro. METHODS: The morphotype, biosynthetic phenotype and cytoskeleton organization of human umbilical vein endothelial cells (HUVEC) were analyzed on supports of variable mechanical resistance. RESULTS: Western blot analysis revealed a strong reduction of the expression of actin and focal-adhesion plaque (FAP) proteins in HUVEC organized in tube-like structures (TLS) on soft matrigel or on matrigel co-polymerized with heat-denatured collagen as compared to HUVEC remaining in a monolayer pattern on rigid matrigel-coat or on matrigel co-polymerized with type I collagen. Human skin fibroblasts morphotype was not altered in these culture conditions and the pattern of FAP proteins and actin was not modulated. By using polyacrylamide gels polymerized with various concentrations of bis-acrylamide to modulate the mechanical resistance of the support and cross-linked to a constant amount of gelatin to provide an equal density of attachment sites, it was shown that the less rigid the support, the more endothelial cells switched to a tube-like pattern. Collagen type I-induced tubulogenesis was accompanied by a profound and reversible remodeling of the actin-FAP complex suggesting a weakening of the bridging between extracellular matrix (ECM) and the cytoskeleton. Human skin fibroblasts and smooth muscle cells, used as control cells, adhered strongly to the collagen, did not form TLS and their network of actin stress fibers was not remodeled. The inhibition of collagen type I-induced tubulogenesis by agents altering the actin cytoskeleton-FAP complex including calpain type I inhibitor, orthovanadate, KT5720 and jasplakinolide, further supports the determinant role of mechanical coupling between the cells and the matrix in tubulogenesis. CONCLUSIONS: A reduced tension between the endothelial cells and the extracellular matrix, originating in the support or within the cells is sufficient to trigger an intracellular signaling cascade leading to tubulogenesis, an event mimicking one of the last steps of angiogenesis.


Subject(s)
Cytoskeleton/physiology , Endothelium, Vascular/physiology , Extracellular Matrix/physiology , Neovascularization, Physiologic/physiology , Blotting, Western/methods , Capillaries , Cell Adhesion/physiology , Cells, Cultured , Collagen , Drug Combinations , Endothelium, Vascular/ultrastructure , Fibroblasts , Humans , Laminin , Microscopy, Phase-Contrast , Proteoglycans , Stress, Mechanical , Umbilical Veins
7.
J Agric Food Chem ; 48(9): 3767-73, 2000 Sep.
Article in English | MEDLINE | ID: mdl-10995268

ABSTRACT

Commercial durum wheat (Triticum durum desf.) semolina was fractionated into starch, gluten, and water extractables. Starch surface proteins and surface lipids were removed, and two starches with manipulated granule size distributions were produced to influence starch properties, affecting its interaction with other semolina components. Reconstituted spaghetti was made with untreated (control) or treated starches. The pasta made from the starting semolina material had lower cooking time and was of lower quality than the samples made from reconstituted material. This was not due to changes in gluten properties as a result of the first step of the fractionation process. For the reconstituted samples, starch interaction behavior was not changed after surface protein or surface lipid removal. Starch surface properties thus do not influence the starch interaction behavior, indicating that starch-gluten interaction in raw (uncooked) pasta is mainly due to physical inclusion. All reconstituted pasta samples also had generally the same cooking quality. It was concluded that the small changes in starch gelatinization behavior, caused by the above-mentioned starch modifications, are of little importance for pasta quality.


Subject(s)
Food , Glutens/chemistry , Starch/chemistry , Cooking , Triticum/chemistry
8.
Breast Cancer Res Treat ; 60(1): 15-28, 2000 Mar.
Article in English | MEDLINE | ID: mdl-10845805

ABSTRACT

The transforming properties of fibroblast growth factor 3 (FGF-3) were investigated in MCF7 breast cancer cells and compared to those of FGF-4, a known oncogenic product. The short form of fgf-3 and the fgf-4 sequences were each introduced with retroviral vectors and the proteins were only detected in the cytoplasm of the infected cells, as expected. In vitro, cells producing FGF-3 (MCF7.fgf-3) and FGF-4 (MCF7.fgf-4) displayed an amount of estrogen receptors decreased to around 45% of the control value. However, MCF7.fgf-3 cell proliferation remained responsive to estradiol supply. The sensitivity of the MCF7.fgf-4 cells, if existant, was masked by the important mitogenic action exerted by FGF-4. In vivo, the MCF7.fgf-3 and MCF7.fgf-4 cells gave rise to tumors under conditions in which the control cells were not tumorigenic. Supplementing the mice with estrogen had the paradoxical effect of totally suppressing the start of the FGF-3 as well as the FGF-4 tumors. Tumorigenicity in the presence of matrigel was similar for MCF7.fgf-3 and control cells and was increased by estrogen supplementation. Once started, the MCF7.fgf-4 tumors grew with a characteristic high rate. Remarkably, FGF-4 but not FGF-3, stimulated the secretion of vascular endothelial growth factor (VEGF165) without altering the steady-state level of its mRNA, suggesting a possible regulation of VEGF synthesis at the translational level in MCF7 cells. The increased VEGF secretion is probably involved in the more aggressive phenotype of the MCF7.fgf-4 cells while a decreased dependence upon micro-environmental factors might be part of the increased tumorigenic potential of the MCF7.fgf-3 cells.


Subject(s)
Breast Neoplasms/pathology , Fibroblast Growth Factors/metabolism , Proto-Oncogene Proteins/metabolism , Animals , Blotting, Western , Breast Neoplasms/genetics , Breast Neoplasms/metabolism , Cadherins/genetics , Cadherins/metabolism , Carcinogenicity Tests , Cell Division , DNA Primers , Endothelial Growth Factors/genetics , Endothelial Growth Factors/metabolism , Female , Fibroblast Growth Factor 3 , Fibroblast Growth Factor 4 , Fibroblast Growth Factors/genetics , Fluorescent Antibody Technique , Gene Expression Regulation, Neoplastic , Humans , Lymphokines/genetics , Lymphokines/metabolism , Mice , Mice, Nude , Proto-Oncogene Proteins/genetics , Receptors, Estradiol/genetics , Receptors, Estradiol/metabolism , Receptors, Progesterone/genetics , Receptors, Progesterone/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Tumor Cells, Cultured/metabolism , Vascular Endothelial Growth Factor A , Vascular Endothelial Growth Factors
9.
Lab Invest ; 78(10): 1291-9, 1998 Oct.
Article in English | MEDLINE | ID: mdl-9800955

ABSTRACT

Angiogenesis is a complex phenomenon likely to be under the strict control of a group of transcription factor(s). Homeobox (HOX)-containing proteins have been identified as regulators controlling the coordinated expression of genes involved in organ development and tissue differentiation. In this study, we have demonstrated that human umbilical vein endothelial cells (HUVEC) express 8 of the 10 HOX genes contained in cluster B. Treatment of HUVEC with tissue plasminogen activator (TPA), an agent known to induce morphologic changes in endothelial cells, or vascular endothelium growth factor (VEGF), a proliferative and angiogenesis inducer, results in a specific time-dependent modulation of the eight HOX genes identified. Interestingly, neither basic fibroblast growth factor, an endothelial proliferative agent, nor TNP-470, a fumagillin derivative with potent antiendothelial cell proliferation properties, affected expression of these HOX genes. Specific modulation of HOX genes by differentiating agents but not by proliferative or antiproliferative molecules suggests that they could be involved in the control of the genetic program that coordinates the construction of new blood vessels.


Subject(s)
Endothelium, Vascular/metabolism , Gene Expression Regulation/physiology , Genes, Homeobox , Multigene Family , Base Sequence , Cell Division/drug effects , Cyclohexanes , Endothelial Growth Factors/pharmacology , Endothelium, Vascular/cytology , Endothelium, Vascular/drug effects , Fibroblast Growth Factor 2/pharmacology , Gene Expression Regulation/drug effects , Humans , Lymphokines/pharmacology , Molecular Sequence Data , Neovascularization, Physiologic/drug effects , Neovascularization, Physiologic/genetics , Neovascularization, Physiologic/physiology , O-(Chloroacetylcarbamoyl)fumagillol , Sesquiterpenes/pharmacology , Tetradecanoylphorbol Acetate/pharmacology , Tissue Plasminogen Activator/pharmacology , Transcription, Genetic/drug effects , Umbilical Cord , Vascular Endothelial Growth Factor A , Vascular Endothelial Growth Factors
10.
Cancer Res ; 57(24): 5590-7, 1997 Dec 15.
Article in English | MEDLINE | ID: mdl-9407972

ABSTRACT

The infection of normal mouse mammary EF43 cells by a retroviral vector carrying either Fgf-3 (EF43.Fgf-3) or Fgf-4 (EF43.Fgf-4) cDNA resulted in the transformation of cells displaying different tumorigenic potentials in nude mice (A. Hajitou and C-M. Calberg-Bacq, Int. J. Cancer, 63: 702-709, 1995). EF43.Fgf-4 produced rapidly developing tumors at all sites of inoculation, whereas EF43.Fgf-3 produced slowly growing tumors only in the mammary fat pad. Cells infected with the vector carrying the selection gene alone (EF43.C) were not tumorigenic. The angiogenic properties of these cells were tested in an in vitro angiogenesis model using human umbilical vein endothelial cells (HUVECs) cultured at the surface of a type I collagen gel and their capacity to form tube-like structures on invasion of the gel. Only the conditioned medium (CM) of EF43.Fgf-4 induced an angiogenic morphotype in HUVECs. In parallel, the mRNA expression of matrix metalloproteinase 1 and c-ETS-1 was increased in the HUVECs displaying a differentiated phenotype, whereas the tissue inhibitor of matrix metalloproteinase 1 mRNA level was decreased. Recombinant human fibroblast growth factor 4 (FGF-4) did not induce an angiogenic phenotype in HUVECs by itself. By Western blot analysis, a high expression of vascular endothelial growth factor (VEGF) was detected in the EF43.Fgf-4 CM. This result was confirmed by Northern blot analysis of total RNA extracted from the three cell types; the steady-state level of VEGF mRNA was low and equivalent in EF43.C and EF43.Fgf-3, whereas it was strongly increased in EF43.Fgf-4. Culturing EF43 cells carrying only the selection gene with increasing concentrations of recombinant human FGF-4 resulted in a dose-dependent stimulation of VEGF. The induction of the angiogenic morphotype and the parallel modulations of the biosynthetic phenotype in HUVECs were completely suppressed by adding a neutralizing antibody directed against VEGF to EF43.Fgf-4 CM. Furthermore, inhibition of protein kinase C by bisindoylmaleimide suppressed the angiogenic phenotype induced by the CM of EF43.Fgf-4. Our results point to an indirect angiogenic activity of FGF-4 through the autocrine induction of VEGF secretion by EF43.Fgf-4 cells, an original signaling pathway that might be significant in tumor progression and metastasis.


Subject(s)
Endothelial Growth Factors/physiology , Fibroblast Growth Factors/physiology , Lymphokines/physiology , Neovascularization, Physiologic/physiology , Animals , Cells, Cultured , Collagen , Culture Media , Endothelial Growth Factors/biosynthesis , Endothelium, Vascular/metabolism , Fibroblast Growth Factor 3 , Fibroblast Growth Factors/biosynthesis , Fibroblast Growth Factors/genetics , Humans , Lymphokines/biosynthesis , Mice , Phenotype , Protein Kinase C/physiology , Proto-Oncogene Proteins/biosynthesis , Proto-Oncogene Proteins/genetics , Proto-Oncogene Proteins/physiology , Up-Regulation/physiology , Vascular Endothelial Growth Factor A , Vascular Endothelial Growth Factors
11.
Exp Cell Res ; 224(2): 215-23, 1996 May 01.
Article in English | MEDLINE | ID: mdl-8612698

ABSTRACT

A model of collagen-induced in vitro angiogenesis was used to investigate the modulation of expression and assembly of focal adhesion plaque-associated proteins during the process of differentiation. Human umbilical vein endothelial cells (HUVEC), first attached on an adhesive substratum (gelatin-, fibronectin-, or laminin-coated dish) or adherent collagen gel and then covered by an overlaying collagen get, organized within 3-4 days in tube-like structures (TLS). Removing the overlaying collagen gel from fully differentiated HUVEC induced a reversion of the process and HUVEC returned to a monolayer pattern. Modulations of focal adhesion-associated proteins occurring in HUVEC during the in vitro differentiation process and its reversal were investigated by Western blot analysis. A significant decrease of expression of vinculin, the integrin alpha2 subunit, talin, alpha-actinin, and actin was observed in TLS whereas the amount of FVIII-related antigen did not vary as compared to control monolayer cultures. During reversal, all the reduced proteins were markedly reexpressed. Human skin fibroblasts (HSF), submitted to the same experimental conditions, did not form TLS. Most of the focal adhesion proteins in HSF were similarly modulated by an overlaying collagen gel with the exception of vinculin, which was not modified. This particular protein was therefore more thoroughly investigated. In a nondifferentiated monolayer of HUVEC, a significant proportion of vinculin was organized into a detergent-resistant juxtamembranous structure (focal adhesion plaque) which disassembled early in TLS formation and reassembled during the reversal of the process. The reduction of vinculin during TLS formation was preceded by a downregulation of its mRNA while this mRNA was upregulated during reversal of the morphotype. These results suggest that the modulations of the cytoskeletal and focal adhesion proteins and more specifically of vinculin coupled to its subcellular redistribution are critical and early events in the cascade of mechanochemical signaling during in vitro angiogenesis induced by fibrillar collagen.


Subject(s)
Collagen/pharmacology , Neovascularization, Physiologic/genetics , Vinculin/genetics , Actins/metabolism , Blotting, Northern , Blotting, Western , Cell Adhesion/genetics , Cell Adhesion Molecules/drug effects , Cell Adhesion Molecules/metabolism , Cell Differentiation/drug effects , Cell Differentiation/physiology , Cytoskeleton/drug effects , Cytoskeleton/metabolism , Endothelium/blood supply , Endothelium/metabolism , Epidermal Cells , Fibroblasts/metabolism , Gene Expression/drug effects , Gene Expression/physiology , Humans , Neovascularization, Physiologic/drug effects , Polymers/pharmacology , RNA, Messenger/metabolism , Umbilical Veins/cytology , Vinculin/metabolism
12.
J Biol Chem ; 267(8): 5217-21, 1992 Mar 15.
Article in English | MEDLINE | ID: mdl-1544904

ABSTRACT

The alpha-amylase excreted by the antarctic bacterium Alteromonas haloplanctis was purified and the corresponding amy gene was cloned and sequenced. N- and C-terminal amino acid sequencing were used to establish the primary structure of the mature A. haloplanctis alpha-amylase which is composed of 453 amino acids with a predicted Mr of 49,340 and a pI of 5.5. Three Ca2+ ions are bound per molecule and its activity is modulated by chloride ions. Within the four consensus sequences, Asp-174, Glu-200, and Asp-264 are the proposed catalytic residues. The psychrotrophic A. haloplanctis alpha-amylase is characterized by a high amylolytic activity at low temperatures, a reduced apparent optimal temperature, and typical thermodynamic activation parameters A. haloplanctis alpha-amylase has also a low thermal stability as demonstrated by the temperature effect on both activity and secondary structure. It is suggested that structure flexibility and lower sensitivity of secondary structure to temperature variations in the low temperature range are the main structural adaptations of the psychrotrophic enzyme. The unusual stacking of small amino acids around the catalytic residues is proposed as a factor inducing active site flexibility and concomitant high activity of the enzyme at low temperatures.


Subject(s)
Genes, Bacterial , Gram-Negative Aerobic Bacteria/enzymology , alpha-Amylases/metabolism , Amino Acid Sequence , Base Sequence , Circular Dichroism , Cloning, Molecular , Enzyme Stability , Gram-Negative Aerobic Bacteria/genetics , Hot Temperature , Kinetics , Molecular Sequence Data , Molecular Weight , Protein Conformation , Restriction Mapping , Thermodynamics , alpha-Amylases/genetics , alpha-Amylases/isolation & purification
13.
J Anim Sci ; 69(10): 4007-18, 1991 Oct.
Article in English | MEDLINE | ID: mdl-1778814

ABSTRACT

The dose related effects of recombinant porcine somatotropin (rpST) on growth, carcass characteristics, muscle properties, and meat quality were investigated in lean Belgian Landrace finishing pigs. Ninety-six pigs (48 barrows and 48 gilts) were injected daily with either vehicle, 1.5, 3, or 6 mg of rpST from 60 to 97.5 kg live weight. Each treatment group consisted of six pens of four pigs each (two of each sex). Pigs were given ad libitum access to a high-protein (20.4% CP) cereal-based diet. Administration of rpST increased (P less than .05) growth rate (16.3 to 25.4%) and improved (P less than .05) feed efficiency (16.9 to 29.4%). Feed consumption was reduced (12%; P less than .05) only in the 6 mg of rpST group. Liver, kidney, and heart weights were increased (P less than .05) in the 3 and 6 mg of rpST groups. Although the Belgian Landrace pigs are bred for superior carcass quality, rpST further improved (P less than .05) carcass composition at all dose levels as evidenced by a reduction (10 to 50%) in a number of subcutaneous fat depth measurements, an increase (10 to 20%) in longissimus muscle area, and an improvement in the lean cut:fat cut ratio. Rate of pH decline in the gluteus and longissimus muscles was similar, but rapid, in all groups (pH after 30 min = 5.74 to 5.94); the ultimate (24 or 72 h) pH was .15 to .2 pH units higher (P less than .05) in the pigs that received the 3 and 6 mg of rpST doses.(ABSTRACT TRUNCATED AT 250 WORDS)


Subject(s)
Growth Hormone/pharmacology , Meat/standards , Muscles/chemistry , Swine/growth & development , Adipose Tissue/drug effects , Adipose Tissue/growth & development , Animals , Body Composition/drug effects , Dose-Response Relationship, Drug , Eating/drug effects , Female , Male , Muscle Development , Muscle Proteins/analysis , Muscles/drug effects , Recombinant Proteins/pharmacology , Water/metabolism , Weight Gain/drug effects
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