ABSTRACT
Tumour necrosis factor alpha (TNF alpha) is an important cytokine in immune regulation and resistance to various micro-organisms. It provides signals to the target cells through two different receptors: TNFR1 and TNFR2. The present report reviews the role of TNF receptors (TNFRs) in the immune response against protozoan parasite infections of medical interest (Toxoplasma gondii, Leishmania major, Trypanosoma cruzi, Plasmodium spp.). TNF alpha has been regarded as a modulator cytokine in host defence against protozoans infections and recent findings on experimental gene-deficient mice have showed that TNF alpha/TNFRs pathway may be beneficial for host protection during these infections.
Subject(s)
Chagas Disease/immunology , Coccidiosis/immunology , Receptors, Tumor Necrosis Factor/physiology , Animals , Humans , Leishmania major , Malaria/immunology , Plasmodium , Toxoplasma , Toxoplasmosis/immunology , Trypanosoma cruziABSTRACT
Here, we review the interactions between parasites and chemokines and chemokine receptors in toxoplasmosis, trypanosomiasis, leishmaniasis, malaria and other diseases caused by protozoan parasites. The potential roles of chemokines after infection by these intracellular pathogens include host defence functions such as leukocyte recruitment, participation in cell-mediated immunity and antiprotozoal activity. However, these interactions can also help the parasite in, for example, the penetration of host cells.
Subject(s)
Chemokines/physiology , Eukaryota/physiology , Host-Parasite Interactions , Amebiasis/immunology , Amebiasis/physiopathology , Animals , Cryptosporidiosis/immunology , Cryptosporidiosis/physiopathology , Immunity, Cellular , Leishmaniasis/immunology , Leishmaniasis/physiopathology , Malaria/immunology , Malaria/physiopathology , Toxoplasmosis/immunology , Toxoplasmosis/physiopathology , Trichomonas Infections/immunology , Trichomonas Infections/physiopathology , Trypanosomiasis/immunology , Trypanosomiasis/physiopathologyABSTRACT
Previous reports have shown that tumor necrosis factor alpha (TNF-alpha) controls the functions of fibroblasts cells, such as proliferation, cell migration and secretion of factors. The signaling for the biological effects of this and other cytokines is usually exerted through cell surface receptors. In this study, we demonstrated the presence of a surface and soluble TNF receptor in MRC5 fibroblasts. The presence of TNFRI and TNFRII mRNA was demonstrated by reverse-transcriptase-PCR (RT-PCR). Both surface TNFRI and TNFRII are expressed and the number of both membrane receptors is 9,251 sites per cell. Using TNF receptor specific antibodies and flow cytometry, we showed that MRC5 cells express mainly TNFRI.
