ABSTRACT
In tribal part of central India burn remains a major cause of morbidity and mortality. Burn management is carried out in conventional manner in most places. The study carried out at Chhatisgarh Institute of Medical Sciences, Bilaspur was Intended to evaluate the efficacy and outcome of 0.5% gentian violet paint local application over conventional dressing treatment of burn wound. The study encompasses 400 patients of burn of varied aetiology admitted in burn ward whose total body surface area of burn was 15% to 50%. The patients receiving conventional treatment were in group I (n=200), and those with gentian violet paint local application formed group II (n= 200). Although, fibrosis of the burn wound, hypertrophic scar were slightly higher in second group it was observed gentian violet paint local application, healed it in 6-8 weeks without severe sepsis and need for skin grafting. From the study it can be concluded that markedly inexpensive gentian violet paint is an useful alternative, for burn wound management.
Subject(s)
Burns/drug therapy , Gentian Violet/administration & dosage , Wound Healing/drug effects , Administration, Topical , Adolescent , Adult , Anti-Infective Agents, Local/administration & dosage , Child , Female , Follow-Up Studies , Humans , Male , Prospective Studies , Treatment Outcome , Young AdultABSTRACT
Blast lung is a clinical condition which is characterised by respiratory difficulty and hypoxia without obvious external injury to the chest in bomb blast cases. Two patients of the bomb blast episode were brought at emergency department at Chhattisgarh Institute of Medical Sciences, Bilaspur in a state of shock. After proper wound care, resuscitation and investigation both the cases proved to be that of blast lung. In bomb blast cases although there might not be any external injury over chest wall, it will be sensible to rule out blast lung in all cases with the help of computerised tomography.
Subject(s)
Blast Injuries/therapy , Lung Injury/therapy , Blast Injuries/complications , Blast Injuries/diagnostic imaging , Child , Humans , Lung Injury/complications , Lung Injury/diagnostic imaging , Shock/etiology , Shock/therapy , Tomography, X-Ray Computed , Young AdultABSTRACT
Lactate dehydrogenase isoenzyme patterns were obtained in the seman of 93 male partners of infertile couples and 28 proven fertile subjects as a control group. Sperm mitochondrial activity index (SMAI) alongwith the conventional seminal parameters was studied for all the subjects excepting the azoospermic and vasectomised males. Only LDH-C4, a germ cell specific isoenzyme activity varied with the variation in sperm density. LDH-C4 activity varied significantly (p<0.001) within and between different groups. Lactate dehydrogenase-C4 activity was absent in 17 azoospermic samples, confirming its germinal epithelial origin, as well as in 8 samples of vasectomised males. In one azoospermic sample, there were many immature germ cells along with surprisingly high LDH-C4 activity suggesting more activity of germinal epithelium associated with high LDH-C4 activity. LDH-C4 activity was reduced significantly in oligozoospermic samples in proportion to sperm density, thus confirming strong correlation (p<0.001) between LDH-C4 and sperm density. There was statistically significant correlation between LDH-C4 and percentage sperm motility as well as between LDH-C4 and Sperm Mitochondrial Activity Index (SMAI) (probability varying from p<0.05 to p<0.01 in different groups), but no such correlation was found between LDH-C4 and sperm morphology. The data confirms LDH-C4 as a germinal epithelial marker. Its relationship with percentage sperm motility is suggestive of definite role of LDH-C4 in evaluation of the spermatozoal quality, similarly its relationship with Sperm Mitochondrial Activity Index (SMAI score) suggest the role of LDH-C4 in metabolism of the spermatocytes and sperms, though further studies are required for clear and detailed understanding of its metabolic role in semen.
Subject(s)
Infertility, Male/enzymology , Isoenzymes/metabolism , L-Lactate Dehydrogenase/metabolism , Mitochondria/enzymology , Spermatozoa/enzymology , Analysis of Variance , Humans , Male , Oligospermia/enzymology , Reference Values , Sperm Count , Sperm Motility/physiologyABSTRACT
The role of male factors in abortions has not been studied extensively. We undertook this study to determine if there was any relationship between hypo-osmotic swelling test score and recurrent abortions. This was a cross-sectional case control study conducted at the semen analysis laboratory at a tertiary level referral centre. The male partners of 25 patients who had three or more first trimester abortions of unknown etiology were chosen as cases. Controls were 26 unmarried volunteers with unknown fertility potential to represent the entire population. The conventional seminal parameters were studied according to WHO guidelines. The HOS test score were obtained by the method advocated by Jayendran et al. There were no statistically significant differences in mean sperm count, percentage motility and morphology among the cases and controls. We found a low hypo-osmotic swelling test score among the cases [55.7 +/- 1.197 (SEM)] than the controls [69.3 +/- 1.143 (SEM)] which was statistically significant [P < 0.001]. The normal spermatozoal membrane is the prerequisite for the specialized cell-to-cell communications and cell-to-cell binding. In spite of apparently normal semen analysis, subtle membrane defects in the spermatozoa, which could be the cause of defective membrane functions in the embryo effecting miscarriages, can be elucidated by the hypo-osmotic swelling test.
