1.
STAR Protoc
; 4(4): 102576, 2023 Dec 15.
Article
in English
| MEDLINE
| ID: mdl-37733596
ABSTRACT
The lymph node (LN) is a critical biological site for immune maturation after vaccination as it includes several cell populations critical for priming the antibody response. Here, we present a protocol for sampling the LN and isolating cell populations to evaluate immunogens targeting germline cells. We describe steps for media and tube preparation and sample collection using an ultrasound-guided LN fine-needle aspiration procedure. This protocol is safe, quick, low-cost, and less invasive than excisional biopsy. For complete details on the use and execution of this protocol, please refer to Leggat et al. (2022).1.
Subject(s)
Germinal Center , Lymph Nodes , Humans , Biopsy, Fine-Needle , Lymph Nodes/diagnostic imaging , Lymph Nodes/pathology , Vaccination , Ultrasonography, Interventional
2.
AIDS
; 31(15): 2177-2178, 2017 Sep 24.
Article
in English
| MEDLINE
| ID: mdl-28906283