ABSTRACT
Human adenocarcinoma HeLa cells surviving infection with low (10(-9) units), medium (10(-6) units), and high (10(-2) units) influenza B titers were compared to their uninfected precursors and to normal endocervical adenoepithelial and metaplastic cells using Papanikolaou-staining method and immunocytochemistry. Normal primary endocervical and infected HeLa cells surviving infection shared similar morphologic, phenotypic, and divisional patterns that differed drastically from those of uninfected HeLa cells. The number of infected hosts surviving 6-7 days of viral exposure did not change during 3-week follow-up period, and their cyclin E levels suggested that they had been arrested to the G1 phase of the cell cycle by viral stress. Our findings suggest that in addition to apoptosis, nononcogenic viral stress activated the expression of endocervical metaplastic-like motifs in surviving hosts. A mechanism of cell response to nononcogenic viral stress was proposed to explain these findings. We conclude that nononcogenic respiratory viruses specifically target and eliminate abnormal cells ectopically overexpressing appropriate receptors and may complement current treatments of cervical cancer.
Subject(s)
Adenocarcinoma/metabolism , Adenocarcinoma/virology , Cervix Uteri/virology , Epithelial Cells/virology , Influenza B virus/physiology , Uterine Cervical Neoplasms/metabolism , Uterine Cervical Neoplasms/virology , Adenocarcinoma/pathology , Cells, Cultured , Cervix Uteri/cytology , Cervix Uteri/metabolism , Cervix Uteri/pathology , Epithelial Cells/cytology , Epithelial Cells/metabolism , Epithelial Cells/pathology , Female , HeLa Cells , Humans , Immunohistochemistry , Phenotype , Titrimetry , Uterine Cervical Neoplasms/pathologyABSTRACT
The first case of soft tissue Ewing's sarcoma in Greece is presented. The tumour was a circumscribed large mass at the right calf, was radically excised and histologically proved to be small cell malignant neoplasm, performing the characteristic structural and cytological pattern of Ewing's sarcoma. Systematic clinical and radiological examination was negative. Operation was followed by local radiotherapy and systematic chemotherapy and the patient remains disease-free and clinically in perfect health eight months after the operation. The case is presented with special reference to its histological appearance and its treatment.
