ABSTRACT
Arjunic acid and arjunolic acid are main bioactive components of Terminalia arjuna stem bark and reported for various biological activities. In this study, microwave-assisted extraction (MAE) of arjunic and arjunolic acid from stem bark of T. arjuna was investigated with developed and validated HPLC-PDA method, which resulted in the isolation of a novel anticancer molecule i.e. arjunic acid. Effects of several experimental parameters, such as type and volume of extraction solvents, microwave power, microwave extraction time, on the extraction efficiencies of arjunic, and arjunolic acid from stem bark of T. arjuna were evaluated. The optimal extraction conditions identified were 5.0 g quantity of stem bark powder, 20 mL of ethyl acetate, preleaching time 10 min, microwave power 600 W, temperature 65°C, and microwave irradiation time 5 min. The results showed that MAE is a more rapid extraction method with higher yield and lower solvent consumptions than reported methods. The HPLC-PDA analysis method was developed and validated to have good linearity, precision, sensitivity, and accuracy. MAE-HPLC-PDA is a faster, convenient, and appropriate method for isolation and determination of arjunic acid and arjunolic acid in the stem bark of T. arjuna.
Subject(s)
Chromatography, High Pressure Liquid/methods , Plant Bark/chemistry , Plant Extracts/analysis , Plant Extracts/isolation & purification , Terminalia/chemistry , Triterpenes/analysis , Chemical Fractionation , Chromatography, High Pressure Liquid/instrumentation , Microwaves , Plant Bark/radiation effects , Terminalia/radiation effects , Triterpenes/isolation & purificationABSTRACT
Vamana Karma (therapeutic emesis) primarily a Samshodhana Karma (purification procedure) is one of the five Pradhana Karmas (chief procedures) of Panchakarma. It is mentioned in Ayurvedic texts that a person after Samyak Vamana (proper Vamana) experiences lightness of the body, Hrit (precordium), Kantha (throat/voice), and Shirah (head) and weakness. This procedure is effectively used in healthy and ailing persons for purification of body and extraction of Doshas (especially Kapha) in Ayurvedic system. It has been found worth to observe the physiological and biochemical changes during Vamana and after the procedure to understand the effect/safety margins of the procedure in healthy volunteers.
ABSTRACT
Vamana Karma is one of the five Pradhana Karmas of Panchakarma which is successfully used in treating Kaphaj disorders. Panchakarma is also indicated in healthy states. (C.Su. 16/13-16) for Shodhana. Textual references are available in Ayurvedic classics, but the procedure needs to be validated in the modern times when Ahara Shakti, Bala and Agni of the individuals have decreased considerably. So the effect of procedure was observed in 30 healthy volunteers of age group 18 to 60 years. Lakshanik, Vaigiki, Maniki and Antiki Shuddhi were observed and vomitus was analyzed macroscopically, microscopically and chemically.
ABSTRACT
Greeva Stambha (A Vataja Disorder) simulates cervical spondylosis, which is a chronic degenerative condition of the cervical spine. Keeping in view the increasing incidence of this problem in modern society with more of desk- workers; an observational study was conducted on 22 patients of Greeva stambha vis-a-vis cervical spondylosis selected from OPD/IPD of CRIA, Punjabi-Bagh, New-Delhi, satisfying the inclusion criteria. The Vatahara treatment viz. Maha Yogaraj Guggulu 500 mg BD, Panchguna tail for local use and Nadi sweda (Local steam with Dashmoola Kwatha) were given for seven days. The results were assessed on the basis of symptomatic improvement using visual analog scale.
