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Biochem Biophys Res Commun ; 314(1): 249-58, 2004 Jan 30.
Article in English | MEDLINE | ID: mdl-14715273

ABSTRACT

The genomic structure of Tetraodon fluviatilis L29 gene was determined and its promoter activity was analyzed in COS-1 cells and zebrafish embryos. The TfL29 gene comprises four exons and three introns, spanning approximately 1.7kb. The 5(')-upstream 2.2-kb of the first exon contains 10 E-boxes and many putative binding motifs for transcription factors GATA-1, AML-1a, c-Myb, Oct-1, CdxA, and NRF-2. Promoter activity assay showed that the distal 2.2-kb fragment not only had high luciferase activity in COS-1 cells, but also strong and ubiquitous GFP expression in a variety of tissues in zebrafish embryos. On the other hand, there are no TATA or CAAT boxes within a 300-bp region upstream from the transcription initiation site. Although this region has high luciferase activity in COS-1 cells, it is not sufficient to drive GFP expression in zebrafish embryos. In this proximal 300-bp region, there are two E-boxes, two CdxA sites, and one NRF-2 site that is immediately downstream of the transcription start site.


Subject(s)
Gene Expression Regulation , Luminescent Proteins/genetics , Luminescent Proteins/metabolism , Promoter Regions, Genetic , Ribosomal Proteins/chemistry , Ribosomal Proteins/genetics , Sequence Analysis, Protein , Amino Acid Sequence , Animals , Base Sequence , COS Cells , Caenorhabditis elegans , Drosophila , Green Fluorescent Proteins , Humans , Molecular Sequence Data , RNA-Binding Proteins , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/genetics , Recombinant Fusion Proteins/metabolism , Ribosomal Proteins/metabolism , Sequence Alignment , Sequence Homology, Amino Acid , Species Specificity , Structure-Activity Relationship , Tetraodontiformes , Transcriptional Activation , Zebrafish
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