ABSTRACT
The pathological findings and the immunohistochemical and molecular diagnosis of two natural cases of pulmonary adenocarcinoma in goats are described. Ovine pulmonary adenocarcinoma (OPA), a contagious lung tumor, caused by an exogenous Jaagsiekte Sheep Retrovirus (JSRV), is reported commonly in sheep and rarely in goats. The affected lungs had a focally extensive and firm nodule or multiple nodules on the cranioventral region. On the cut surface, the nodules were greyish, granular and moist, resembling classical form of OPA. Microscopically, the lung sections showed unencapsulated nodules of neoplastic epithelial cells, from alveoli and bronchioles, forming papillary projections or acini. On the immunohistochemical analysis, JSRV capsid protein was demonstrated in the neoplastic epithelial cells. Genomic DNA was extracted from the lung tumor tissues and was subjected to U3-hn-PCR that further confirmed the presence of JSRV. The pathological findings in goats were similar to that of OPA affecting lungs of sheep and, to the authors knowledge, this is the first report of the disease in goats with immunohistochemical and PCR confirmation of JSRV.(AU)
Subject(s)
Animals , Ruminants , Adenocarcinoma/diagnosis , Adenocarcinoma/veterinary , Pulmonary Adenomatosis, Ovine/diagnosis , Jaagsiekte sheep retrovirus , Lung/pathology , /veterinary , Immunohistochemistry/veterinaryABSTRACT
The pathological findings and the immunohistochemical and molecular diagnosis of two natural cases of pulmonary adenocarcinoma in goats are described. Ovine pulmonary adenocarcinoma (OPA), a contagious lung tumor, caused by an exogenous Jaagsiekte Sheep Retrovirus (JSRV), is reported commonly in sheep and rarely in goats. The affected lungs had a focally extensive and firm nodule or multiple nodules on the cranioventral region. On the cut surface, the nodules were greyish, granular and moist, resembling classical form of OPA. Microscopically, the lung sections showed unencapsulated nodules of neoplastic epithelial cells, from alveoli and bronchioles, forming papillary projections or acini. On the immunohistochemical analysis, JSRV capsid protein was demonstrated in the neoplastic epithelial cells. Genomic DNA was extracted from the lung tumor tissues and was subjected to U3-hn-PCR that further confirmed the presence of JSRV. The pathological findings in goats were similar to that of OPA affecting lungs of sheep and, to the authors knowledge, this is the first report of the disease in goats with immunohistochemical and PCR confirmation of JSRV.
Subject(s)
Animals , Adenocarcinoma/diagnosis , Adenocarcinoma/veterinary , Pulmonary Adenomatosis, Ovine/diagnosis , Lung/pathology , Jaagsiekte sheep retrovirus , Ruminants , Immunohistochemistry/veterinaryABSTRACT
A nine years old, Graded Murrah she buffalo was presented to local Veterinary dispensary with clinical signs of raised elevations of growths on the pinna of right ear near its base and lateral aspects showing ulceration, necrosis, scales, crusts and scabs. Histopathologically, the tissue sections from the growth revealed groups, islands or cords of neoplastic squamous epithelium with epithelial pearls at some places. By immunohistochemistry, proliferating epithelial cells were positive for cytokeratin and expressed PCNA. Based on gross, histologic and immunohistochemical findings, the growth was diagnosed as cutaneous squamous cell carcinoma of ear (AU)
Subject(s)
Animals , Neoplasms, Squamous Cell/diagnosis , Neoplasms, Squamous Cell/surgery , Neoplasms, Squamous Cell/veterinary , Ear, External/injuries , Ear, External/surgery , Keratins/analysis , Proliferating Cell Nuclear Antigen/analysis , BuffaloesABSTRACT
A nine years old, Graded Murrah she buffalo was presented to local Veterinary dispensary with clinical signs of raised elevations of growths on the pinna of right ear near its base and lateral aspects showing ulceration, necrosis, scales, crusts and scabs. Histopathologically, the tissue sections from the growth revealed groups, islands or cords of neoplastic squamous epithelium with epithelial pearls at some places. By immunohistochemistry, proliferating epithelial cells were positive for cytokeratin and expressed PCNA. Based on gross, histologic and immunohistochemical findings, the growth was diagnosed as cutaneous squamous cell carcinoma of ear