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1.
Clin Diagn Lab Immunol ; 8(6): 1150-5, 2001 Nov.
Article in English | MEDLINE | ID: mdl-11687456

ABSTRACT

An immunochromatographic test that incorporates recombinant antigens (Dengue Duo Rapid Strip Test; PanBio, Brisbane, Australia) has recently become commercially available. This assay is performed in 15 min and detects both immunoglobulin M (IgM) and IgG in a capture format. The four recombinant proteins used represent the N-terminal 80% of the viral envelope glycoproteins of dengue viruses 1, 2, 3, and 4, respectively. The sensitivity and specificity of the recombinant-antigen-based assay were 90 and 86%, respectively. The similar diagnostic performance of these antigens to that of enzyme-linked immunosorbent assays using whole dengue virus suggests that they mimic whole dengue viruses in primary structure and epitope conformation. These results suggest that recombinant proteins can be used in diagnostic assays for dengue to overcome safety issues associated with the use of whole virus.


Subject(s)
Antigens, Viral/immunology , Chromatography/methods , Dengue Virus/isolation & purification , Dengue/diagnosis , Child , Cross Reactions , Dengue/immunology , Dengue Virus/immunology , Encephalitis, Japanese/immunology , Enzyme-Linked Immunosorbent Assay , Humans , Recombinant Proteins/immunology , Sensitivity and Specificity , Serologic Tests , Viral Envelope Proteins/immunology
2.
Clin Diagn Lab Immunol ; 8(2): 409-14, 2001 Mar.
Article in English | MEDLINE | ID: mdl-11238230

ABSTRACT

The variable 56-kDa major outer membrane protein of Orientia tsutsugamushi is the immunodominant antigen in human scrub typhus infections. We developed a rapid immunochromatographic flow assay (RFA) for the detection of immunoglobulin M (IgM) and IgG antibodies to O. tsutsugamushi. The RFA employs a truncated recombinant 56-kDa protein from the Karp strain as the antigen. The performance of the RFA was evaluated with a panel of 321 sera (serial bleedings of 85 individuals suspected of scrub typhus) which were collected in the Pescadore Islands, Taiwan, from 1976 to 1977. Among these 85 individuals, IgM tests were negative for 7 cases by both RFA and indirect fluorescence assay (IFA) using Karp whole-cell antigen. In 29 cases specific responses were detected by the RFA earlier than by IFA, 44 cases had the same detection time, and 5 cases were detected earlier by IFA than by RFA. For IgG responses, 4 individuals were negative with both methods, 37 cases exhibited earlier detection by RFA than IFA, 42 cases were detected at the same time, and 2 cases were detected earlier by IFA than by RFA. The sensitivities of RFA detection of antibody in sera from confirmed cases were 74 and 86% for IgM and IgG, respectively. When IgM and IgG results were combined, the sensitivity was 89%. A panel of 78 individual sera collected from patients with no evidence of scrub typhus was used to evaluate the specificity of the RFA. The specificities of the RFA were 99% for IgM and 97% for IgG. The sensitivities of IFA were 53 and 73% for IgM and IgG, respectively, and were 78% when the results of IgM and IgG were combined. The RFA test was significantly better than the IFA test for the early detection of antibody to scrub typhus in primary infections, while both tests were equally sensitive with reinfected individuals.


Subject(s)
Bacterial Outer Membrane Proteins/immunology , Immunoassay/methods , Orientia tsutsugamushi/isolation & purification , Reagent Strips , Scrub Typhus/diagnosis , Antibody Specificity , Antigens, Bacterial/immunology , Humans , Immunodominant Epitopes/immunology , Immunoglobulin G/blood , Immunoglobulin M/blood , Orientia tsutsugamushi/immunology , Recombinant Proteins/immunology , Sensitivity and Specificity
3.
Clin Diagn Lab Immunol ; 7(5): 850-2, 2000 Sep.
Article in English | MEDLINE | ID: mdl-10973469

ABSTRACT

A commercially available enzyme-linked immunosorbent assay (ELISA) (PanBio Dengue Screening ELISA) that utilized both immunoglobulin M (IgM) and IgG capture in the same microtiter well for the diagnosis of dengue infection was evaluated. Sensitivity in primary and secondary dengue was 95%, while specificity was 94%.


Subject(s)
Antibodies, Viral/blood , Dengue/diagnosis , Immunoglobulin G/blood , Immunoglobulin M/blood , Antibodies, Viral/immunology , Dengue/blood , Dengue/immunology , Enzyme-Linked Immunosorbent Assay/methods , Evaluation Studies as Topic , Humans , Immunoglobulin G/immunology , Immunoglobulin M/immunology , Reagent Kits, Diagnostic , Sensitivity and Specificity
4.
J Clin Microbiol ; 38(7): 2701-5, 2000 Jul.
Article in English | MEDLINE | ID: mdl-10878067

ABSTRACT

The 56-kDa major outer membrane protein antigen of Orientia tsutsugamuchi is the immunodominant antigen in human scrub typhus (ST) infections. An enzyme-linked immunosorbent assay (ELISA) using a recombinant 56-kDa protein (r56) to detect specific immunoglobulin M (IgM) produced in ST infections was developed, and its performance was evaluated using sera from patients with active ST (n = 59), spotted fever (SF) (n = 31), and murine typhus (MT) (n = 6) and from those without rickettsial infection (n = 52). The r56 ELISA was compared to an ELISA using native whole cell lysate of O. tsutsugamushi Karp or O. tsutsugamushi Gilliam as antigens. The performance of the assays using r56 was similar to that of those using native antigens. Using indirect immunoperoxidase (IIP) as the reference test, sensitivities were 86, 88, and 88% while specificities were 84, 90, and 87% in the three assays. Furthermore, cross-reactivity in confirmed cases of SF and MT was low (5.4, 2.7, and 2.7% respectively). The additional use of IgG in the r56 ELISA gave improved performance (sensitivity, 80%; specificity, 96%; cross-reactivity in SF and MT, 2.7%). The detection of high levels of IgG in some IgM-negative patients illustrates the importance of including a test for IgG in the detection of secondary or reactivated infections, since many of these patients were from regions in Thailand where these infections are endemic.


Subject(s)
Antibodies, Bacterial/blood , Antigens, Surface/immunology , Enzyme-Linked Immunosorbent Assay/methods , Orientia tsutsugamushi/immunology , Scrub Typhus/diagnosis , Antigens, Surface/genetics , Bacterial Outer Membrane Proteins/genetics , Bacterial Outer Membrane Proteins/immunology , Cross Reactions , Evaluation Studies as Topic , Humans , Immunoenzyme Techniques , Reagent Kits, Diagnostic , Recombinant Proteins/genetics , Recombinant Proteins/immunology , Rickettsia Infections/diagnosis , Rickettsia Infections/immunology , Scrub Typhus/immunology , Sensitivity and Specificity
5.
J Clin Microbiol ; 38(4): 1645-7, 2000 Apr.
Article in English | MEDLINE | ID: mdl-10747159

ABSTRACT

A commercially available enzyme-linked immunosorbent assay (ELISA) for the diagnosis of Q fever (PanBio Coxiella burnetii immunoglobulin M [IgM] ELISA, QFM-200) was compared to the indirect fluorescent antibody test (IFAT) for C. burnetii IgM and the complement fixation test (CFT). The ELISA demonstrated 92% agreement with the reference method (IFAT), and gave a sensitivity of 99% (69 of 70 samples) and a specificity of 88% (106 of 121). Specificity can be increased with confirmation by IFAT. CFT was found to have a specificity of 90% (107 of 119), although it was lacking in sensitivity (73%; 51 of 70). No cross-reactivity was observed in the ELISA with serum samples from patients with mycoplasma (n = 6), chlamydia (n = 5), or legionella (n = 4) infections, although 2 of 5 patients with leptospirosis and 1 of 4 samples containing rheumatoid factor (RF) demonstrated positive results in the ELISA. Results indicate that the performance of the PanBio C. burnetii (Q fever) IgM ELISA (F = 187) is superior to that of CFT (F = 163), and consequently the ELISA should be a useful aid in the diagnosis of acute Q fever.


Subject(s)
Coxiella burnetii/immunology , Enzyme-Linked Immunosorbent Assay , Immunoglobulin M/blood , Q Fever/diagnosis , Antibodies, Bacterial/blood , Complement Fixation Tests , Fluorescent Antibody Technique, Indirect , Humans , Q Fever/microbiology , Sensitivity and Specificity
6.
J Clin Microbiol ; 38(4): 1670-1, 2000 Apr.
Article in English | MEDLINE | ID: mdl-10747166

ABSTRACT

An immunochromatographic test for the rapid determination of immunoglobulin M (IgM) and IgG antibodies to Burkholderia pseudomallei was evaluated by using sera from bacteriologically confirmed melioidosis patients and high-risk and clinically suspected patients, along with disease control groups. The sensitivities were 100 and 93% for the IgG and IgM tests, respectively, while the specificity was 95% for both assays. The test was rapid and simple to perform, with results obtained in 10 min.


Subject(s)
Antibodies, Bacterial/blood , Burkholderia pseudomallei/immunology , Immunoassay/methods , Melioidosis/diagnosis , Chromatography/methods , Evaluation Studies as Topic , Fluorescent Antibody Technique, Indirect , Humans , Immunoglobulin G/blood , Immunoglobulin M/blood , Melioidosis/microbiology , Reagent Kits, Diagnostic , Sensitivity and Specificity
7.
J Clin Virol ; 16(2): 135-44, 2000 Apr.
Article in English | MEDLINE | ID: mdl-10720818

ABSTRACT

BACKGROUND: A number of commercial ELISA for dengue diagnosis have recently become available, though direct comparison between these assays have not been published. OBJECTIVES: The Venture Technologies Dengue IgM and IgG Dot Blot assays and the PanBio Dengue Duo IgM and IgG Capture ELISA were compared. STUDY DESIGN: Paired sera from patients with dengue (n=20) and Japanese encephalitis (JE, n=10), and single sera from patients with typhoid (n=10), leptospirosis (n=10) and scrub typhus (n=10) were assayed according to the manufacturer's instructions. RESULTS: The Dot Blot IgM ELISA showed higher sensitivity than the PanBio IgM ELISA (100 vs. 95%), while the PanBio IgM ELISA showed higher specificity in JE (100 vs. 20%) and non-flavivirus infections (100 vs. 97%). Defining elevation of either IgM or IgG as a positive result, the Dot Blot and ELISA tests both showed 100% sensitivity in dengue infection, while the PanBio test showed superior specificity in JE (70 vs. 0%) and non-flavivirus infections (100 vs. 67%). CONCLUSIONS: Both assays are useful aids to the serological diagnosis of dengue infection. The clinical setting, user preference and local conditions will be important in determining which test is more appropriate.


Subject(s)
Antibodies, Viral/immunology , Dengue/diagnosis , Enzyme-Linked Immunosorbent Assay/methods , Immunoblotting/methods , Antibodies, Viral/blood , Child , Dengue/blood , Dengue/immunology , Humans , Immunoglobulin G/blood , Immunoglobulin G/immunology , Immunoglobulin M/blood , Immunoglobulin M/immunology , Sensitivity and Specificity , Serologic Tests/methods
8.
J Clin Microbiol ; 37(11): 3738-41, 1999 Nov.
Article in English | MEDLINE | ID: mdl-10523589

ABSTRACT

A new commercial enzyme-linked immunosorbent assay (ELISA) for the diagnosis of Japanese encephalitis virus infections showed a sensitivity of 88% with sera and 81% with cerebrospinal fluid and a specificity of 97% with sera from patients with primary and secondary dengue virus infections. Specificity was 100% when samples from nonflavivirus infections were tested.


Subject(s)
Encephalitis, Japanese/diagnosis , Enzyme-Linked Immunosorbent Assay/methods , Antibodies, Viral/blood , Antibodies, Viral/cerebrospinal fluid , Dengue/diagnosis , Dengue/immunology , Encephalitis Virus, Japanese/immunology , Encephalitis, Japanese/immunology , Enzyme-Linked Immunosorbent Assay/statistics & numerical data , Evaluation Studies as Topic , Humans , Immunoglobulin M/blood , Immunoglobulin M/cerebrospinal fluid , Sensitivity and Specificity
9.
Clin Diagn Lab Immunol ; 6(5): 705-12, 1999 Sep.
Article in English | MEDLINE | ID: mdl-10473522

ABSTRACT

The performances of the MRL dengue fever virus immunoglobulin M (IgM) capture enzyme-linked immunosorbent assay (ELISA) and the PanBio Dengue Duo IgM capture and IgG capture ELISA were compared. Eighty sera from patients with dengue virus infections, 24 sera from patients with Japanese encephalitis (JE), and 78 sera from patients with nonflavivirus infections, such as malaria, typhoid, leptospirosis, and scrub typhus, were used. The MRL test showed superior sensitivity for dengue virus infections (94 versus 89%), while the PanBio test showed superior specificity for JE (79 versus 25%) and other infections (100 versus 91%). The PanBio ELISA showed better overall performance, as assessed by the sum of sensitivity and specificity (F value). When dengue virus and nonflavivirus infections were compared, F values of 189 and 185 were obtained for the PanBio and MRL tests, respectively, while when dengue virus infections and JE were compared, F values of 168 and 119 were obtained. The results obtained with individual sera in the PanBio and MRL IgM ELISAs showed good correlation, but this analysis revealed that the cutoff value of the MRL test was set well below that of the PanBio test. Comparing the sensitivity and specificity of the tests at different cutoff values (receiver-operator analysis) revealed that the MRL and PanBio IgM ELISAs performed similarly in distinguishing dengue virus from nonflavivirus infections, although the PanBio IgM ELISA showed significantly better distinction between dengue virus infections and JE. The implications of these findings for the laboratory diagnosis of dengue are discussed.


Subject(s)
Dengue Virus/isolation & purification , Dengue/diagnosis , Enzyme-Linked Immunosorbent Assay/methods , Immunoglobulin M/analysis , Adolescent , Adult , Alphavirus Infections/diagnosis , Alphavirus Infections/immunology , Antibodies, Viral/analysis , Antibody Specificity , Asia, Southeastern , Chikungunya virus/immunology , Child , Child, Preschool , Dengue/immunology , Dengue Virus/immunology , Diagnosis, Differential , Encephalitis Virus, Japanese/immunology , Encephalitis, Japanese/diagnosis , Encephalitis, Japanese/immunology , Female , Humans , Immunoglobulin G/analysis , Infant , Male , Sensitivity and Specificity
10.
Am J Trop Med Hyg ; 60(4): 693-8, 1999 Apr.
Article in English | MEDLINE | ID: mdl-10348250

ABSTRACT

A commercial capture ELISA for specific IgM and IgG antibodies produced during dengue infection (PanBio Dengue Duo) showed excellent sensitivity (99%, n = 78) using sera collected at hospital discharge compared with established ELISA and hemagglutination inhibition (HAI) assays. Furthermore, the ELISA was able to diagnose 79% of the dengue cases using sera collected at hospital admission. The ELISA also showed high specificity (92%) in paired sera from patients without flavivirus infection (n = 26), although 45% of the patients with Japanese encephalitis (n = 20) showed elevation of IgG but not IgM. The IgG capture ELISA showed good correlation with the HAI assay (r = 0.83, P < 0.0001), and IgG levels could be used to distinguish between primary and secondary infection, with 100% of primary infections and 96% of secondary infections being correctly classified. This ELISA should prove useful in the clinical diagnosis of dengue infections.


Subject(s)
Antibodies, Viral/blood , Dengue Virus/immunology , Dengue/diagnosis , Enzyme-Linked Immunosorbent Assay/methods , Child , Evaluation Studies as Topic , Hemagglutination Inhibition Tests , Humans , Immunoglobulin G/blood , Immunoglobulin M/blood , Reagent Kits, Diagnostic , Sensitivity and Specificity
12.
Dis Markers ; 14(2): 99-112, 1998 Oct.
Article in English | MEDLINE | ID: mdl-9868597

ABSTRACT

Monoclonal antibody TH1 (IgM) was prepared by immunizing mice with deglycosylated (TFMSA-treated) cystic fibrosis mucin. TH1 reacted strongly with TFMSA treated cystic fibrosis mucin but not with the fully glycosylated mucin, indicating reactivity with a core mucin epitope. TH1 showed no reactivity with ovine mucin (98% of glycans as sialyl-Tn) but reacted strongly with desialylated ovine mucin, indicating the epitope for this mab was the Tn-antigen (O-linked GalNAc). However, TH1 showed no reactivity with Tn-positive red blood cells, and the binding of TH1 was not inhibited by GalNAc at 2.5 mg/ml, illustrating the importance of the peptide sequence to which the GalNAc is attached. TH1 stained the majority of cancers of the colon, lung, stomach, ovary, breast, and cervix, and the cellular distribution of this antigen in normal tissue suggested reactivity with immature mucin. This antibody appears to be a useful reagent for the detection of immature mucin.


Subject(s)
Antibodies, Monoclonal/biosynthesis , Epitopes/immunology , Mucins/immunology , Amino Acid Sequence , Animals , Antibodies, Monoclonal/immunology , Antibody Specificity , Breast Neoplasms/metabolism , Colonic Neoplasms/metabolism , Cystic Fibrosis/metabolism , Enzyme-Linked Immunosorbent Assay , Female , Glycosylation , Hybridomas/immunology , Immunoenzyme Techniques , Mesylates/pharmacology , Mice , Mice, Inbred BALB C , Molecular Sequence Data , Mucins/analysis , Mucins/chemistry , Neoplasms/chemistry , Ovarian Neoplasms/metabolism , Tumor Cells, Cultured
13.
J Clin Microbiol ; 36(12): 3737-9, 1998 Dec.
Article in English | MEDLINE | ID: mdl-9817913

ABSTRACT

Saliva was collected prospectively from patients presenting with suspected dengue infection 4 to 8 days after the onset of symptoms and assayed by a commercial dengue immunoglobulin M (IgM) and IgG capture enzyme-linked immunosorbent assay (ELISA) (PanBio Dengue Duo ELISA). Laboratory diagnosis was based on virus isolation and on hemagglutination inhibition (HAI) assay and an in-house IgM and IgG capture ELISA. With a positive result defined as either salivary IgM or IgG levels above the cutoff value, an overall sensitivity of 92% was obtained for both primary- and secondary-dengue patients (22 of 24), while no patients with non-flavivirus infections (n = 11) and no healthy laboratory donors (n = 17) showed elevation of salivary antidengue antibody (100% specificity). Salivary IgG levels correlated well with serum HAI titer (r = 0.78), and salivary IgG levels could be used to distinguish between primary- and secondary-dengue virus infections.


Subject(s)
Antibodies, Viral/analysis , Dengue Virus/immunology , Dengue/diagnosis , Saliva/virology , Enzyme-Linked Immunosorbent Assay , Humans , Immunoglobulin A, Secretory/analysis , Immunoglobulin G/analysis , Immunoglobulin M/analysis , Prospective Studies , Sensitivity and Specificity
14.
Cancer Immunol Immunother ; 46(5): 245-52, 1998 Jul.
Article in English | MEDLINE | ID: mdl-9690452

ABSTRACT

Antibodies reactive with peptide epitopes on the core protein of MUC1 epithelial mucin have been demonstrated in some patients with adenocarcinomas. Because these epitopes can be exposed on MUC1 in the serum of healthy women, we measured concentrations of MUC1-reactive antibodies in the serum of healthy pregnant and non-pregnant women, and in patients with benign and malignant ovarian tumours. Antibodies were measured in an enzyme-linked immunosorbent assay utilising a synthetic peptide corresponding to a 105-amino-acid segment of the MUC1 tandem repeat region (5.25 repeats). MUC1-reactive antibodies were always of an IgM isotype and concentrations were highest in young healthy women and declined progressively with age (P=0.0006) concomitantly with increasing serum MUC1 levels (P=0.003). Regardless of age, antibody levels were lower in cancer patients than in healthy women (P<0.0001), but MUC1 levels were much higher in cancer patients (P<0.0001). Although high antibody levels were associated with greater survival in ovarian cancer (P=0.015), multivariate regression analysis showed that this was not a significant independent prognostic indicator after consideration of the International Federation of Gynaecology and Obstetrics (FIGO) stage, histological type, serum MUC1 levels and age. Serial measurement of MUC1 and MUC1 antibodies during treatment in 18 patients with ovarian cancer and throughout pregnancy in 10 women showed a negative correlation between alterations in MUC1 and MUC1 antibodies. These results show that MUC1-peptide-reactive antibodies are present in the serum of healthy women and women with cancer and that they probably form immune complexes with MUC1, but provide no evidence for an augmentation of the humoral immune response to MUC1 in ovarian cancer


Subject(s)
Antibodies, Neoplasm/blood , Antibodies/blood , Immunoglobulin M/blood , Mucin-1/blood , Ovarian Neoplasms/blood , Amino Acid Sequence , Antibodies/immunology , Antibodies, Neoplasm/immunology , Antibody Specificity , Antigen-Antibody Complex/blood , Antigens, Neoplasm/blood , Antigens, Neoplasm/immunology , Enzyme-Linked Immunosorbent Assay , Epitopes/immunology , Female , Humans , Immunoglobulin M/immunology , Molecular Sequence Data , Mucin-1/immunology , Ovarian Diseases/blood , Ovarian Diseases/immunology , Ovarian Neoplasms/immunology , Pregnancy , Prognosis , Repetitive Sequences, Nucleic Acid
15.
Clin Diagn Virol ; 10(1): 75-81, 1998 May 01.
Article in English | MEDLINE | ID: mdl-9646004

ABSTRACT

BACKGROUND: Rapid diagnosis of dengue infection is essential to patient management and disease control. The development of a rapid (5 min) immunochromatographic test and a 2 h commercial capture enzyme linked immunosorbent assay (ELISA) for anti-dengue IgM and IgG antibodies may lead to more rapid and accurate testing in peripheral health settings and diagnostic laboratories. OBJECTIVES: Evaluate two new commercial tests for dengue serology (Dengue Rapid test and Dengue Duo ELISA; PanBio, Brisbane, Australia). STUDY DESIGN: The sensitivity and specificity of the tests were compared with in-house dengue IgM ELISA and hemagglutination-inhibition (HAI) assays using known positive and negative dengue specimens, as well as specimens from non-dengue cases. RESULTS: Both assays showed excellent sensitivity in the diagnosis of both primary and secondary dengue infection (100%). In both assays, IgG levels showed excellent correlation with the hemagglutination-inhibition (HAI) assay, and these could be used to distinguish between primary and secondary dengue infections (92 and 97% of patients correctly classified in the rapid test and Duo ELISA, respectively). Specificity in both assays was 89% when sera from patients, with no apparent dengue infection, typhoid, leptospirosis and malaria, were tested. CONCLUSIONS: These tests should be a useful aid in confirming the clinical diagnosis of dengue infection. The rapid test will be particularly valuable in peripheral health settings, while the ELISA has a place in central testing laboratories.


Subject(s)
Antibodies, Viral/blood , Dengue/blood , Enzyme-Linked Immunosorbent Assay/methods , Immunoglobulin G/blood , Immunoglobulin M/blood , Chromatography , Dengue/diagnosis , Dengue/immunology , Hemagglutination Inhibition Tests , Humans , Reagent Kits, Diagnostic , Sensitivity and Specificity
17.
Cancer ; 82(6): 1019-27, 1998 Mar 15.
Article in English | MEDLINE | ID: mdl-9506345

ABSTRACT

BACKGROUND: Controversial findings have been reported regarding the expression of the Thomsen-Friedenreich (TF) antigen in colorectal neoplasms when different monoclonal antibodies (MoAbs) have been used. Moreover, there is no information available regarding the carrier protein(s) of this antigen. METHODS: Forty-five colorectal adenomas and 48 carcinomas were studied by avidin-biotin complex-peroxidase immunohistochemistry. The immunohistochemistry employed the MoAb BW835, which was reactive to a carrier specific and site specific TF antigen on MUC1 mucin, as well as reference antibodies directed to MUC1 (HMFG2) or MUC2 core peptides (4F1) and directed to TF antigen irrespective of its carrier (A78-G/A7, peanut agglutinin). To evaluate the coexpression of different epitopes by the same antigen, sandwich enzyme-linked immunoadsorbent assays were performed. RESULTS: Although MUC1 peptide antigen and MUC1-bound TF antigen were not detectable in normal or transitional mucosa surrounding colorectal neoplasms, expression of these antigens in adenomas accompanied the development of high grade dysplasia. By contrast, MUC2 expression detected by the MoAb 4F1 was inversely correlated with the progression of the adenoma-carcinoma sequence. In well- and moderately differentiated colorectal carcinomas, the neo-expressed TF antigen is predominantly bound to MUC1. This feature could be demonstrated by antigen coexpression using peptide and the TF antigen specific MoAbs. However, in mucinous carcinomas exhibiting a weak MUC1 peptide expression in most specimens, the presence of TF antigen on the MUC2 peptide core cannot be ruled out. CONCLUSIONS: TF antigen is strongly coexpressed with MUC1 mucin peptide core in the colorectal adenoma-carcinoma sequence, resulting in well- and moderately differentiated carcinomas. Only in mucinous carcinomas may it be coexpressed with MUC2 antigen.


Subject(s)
Adenocarcinoma/immunology , Antigens, Tumor-Associated, Carbohydrate/metabolism , Carcinoma/immunology , Colorectal Neoplasms/immunology , Mucin-1/metabolism , Adenocarcinoma/pathology , Antibodies, Monoclonal , Carcinoma/pathology , Colorectal Neoplasms/pathology , Enzyme-Linked Immunosorbent Assay , Epitopes , Humans , Immunohistochemistry , In Vitro Techniques , Mucin-1/immunology , Mucin-2 , Mucins/metabolism
18.
Clin Diagn Lab Immunol ; 5(1): 7-10, 1998 Jan.
Article in English | MEDLINE | ID: mdl-9455871

ABSTRACT

A commercially available capture enzyme-linked immunosorbent assay (ELISA) for the detection of specific immunoglobulin M (IgM) and IgG antibodies produced during dengue infection (PanBio Dengue Duo) was evaluated with paired serum specimens from 176 patients. Diagnosis was based on a hemagglutination inhibition (HAI) assay, with patients having either primary dengue (n = 90), secondary dengue (n = 58), or no dengue (n = 28) infection. The combined use of IgM and IgG (sensitivity, 99%; specificity, 96%) was superior to the use of IgM alone (sensitivity, 88%; specificity, 96%) or IgG alone (sensitivity, 85%; specificity, 96%). Furthermore, with the first serum sample of the pair of serum samples, the ELISA was able to diagnose significantly more cases of dengue than the HAI assay (55% versus 14%). The results of the IgG capture ELISA gave a significant correlation with those of the HAI assay (r = 0.91; P < 0.0001), and the IgG capture ELISA could be used to distinguish between primary and secondary infection. The best distinction was observed when an IgG cutoff ratio of 3.0 was used, with 88% of primary infections and 98% of secondary infections being correctly classified. This ELISA should prove to be useful in the clinical diagnosis of dengue infection.


Subject(s)
Antibodies, Anti-Idiotypic/blood , Dengue/blood , Enzyme-Linked Immunosorbent Assay/methods , Dengue/diagnosis , Dengue/immunology , Hemagglutination Inhibition Tests , Humans , Immunoglobulin G/immunology , Immunoglobulin M/immunology , Sensitivity and Specificity
19.
J Clin Microbiol ; 36(1): 234-8, 1998 Jan.
Article in English | MEDLINE | ID: mdl-9431954

ABSTRACT

A rapid (<7-min) immunochromatographic test for immunoglobulin M (IgM) and IgG antibodies to dengue viruses was evaluated by using hospital admission and discharge sera from 124 patients. The reference laboratory diagnosis was based on the results of virus isolation, hemagglutination-inhibition assay (HAI), and enzyme immunoassay (EIA). By the standard assays, patients experienced primary dengue virus infection (n = 30), secondary dengue virus infection (n = 48), Japanese encephalitis (JE) virus infection (n = 20), or no flavivirus infection (n = 26). The rapid test demonstrated 100% sensitivity in the diagnosis of dengue virus infection and was able to distinguish between primary and secondary dengue virus infections through the separate determinations of IgM and IgG. For all patients with primary dengue virus infection a positive test for IgM to dengue virus and a negative test for IgG to dengue virus were obtained, whereas for 46 of 48 patients (96%) with secondary dengue virus infection, a positive test for IgG to dengue virus with or without a positive test for IgM to dengue virus was obtained. The remaining two patients with secondary dengue virus infection had positive IgM test results and negative IgG test results. Furthermore, the rapid test was positive for patients confirmed to be infected with different dengue virus serotypes (12 infected with dengue virus serotype 1, 4 infected with dengue virus serotype 2, 3 infected with dengue virus serotype 3, and 2 infected with dengue virus serotype 4). The specificity of the test for nonflavivirus infections was 88% (3 of 26 positive), while for JE virus infections the specificity of the test was only 50% (10 of 20). However, most patients with secondary dengue virus infection were positive for both IgM and IgG antibodies to dengue virus, while no patients with JE virus infection had this profile, so cross-reactivity was only a concern for a small proportion of patients with secondary dengue infections. The rapid test demonstrated a good correlation with the reference EIA and HAI and should be useful for the rapid diagnosis of dengue virus infections.


Subject(s)
Antibodies, Viral/blood , Dengue Virus/immunology , Dengue/diagnosis , Hemagglutination Inhibition Tests , Humans , Immunoenzyme Techniques , Immunoglobulin G/blood , Immunoglobulin M/blood , Sensitivity and Specificity
20.
Dis Markers ; 14(3): 169-75, 1998 Nov.
Article in English | MEDLINE | ID: mdl-10427477

ABSTRACT

Amiodarone is used to treat life-threatening cardiac arrhythmias. Amiodarone-induced pulmonary toxicity (APT) can be difficult to diagnose. APT may result in increased mucus production and mucin expression. Thus, serum mucin-1 was evaluated as a marker for amiodarone-induced pulmonary toxicity. Concentrations of mucin-1 in peripheral blood were determined using cancer-associated serum antigen (CASA) assay in patients taking amiodarone. Eight of ten patients who developed major amiodarone toxicity had high serum CASA levels. Patients with toxicity had a significantly higher mean rank CASA concentration compared with those without major toxicity. CASA shows potential as a marker for amiodarone-induced toxicity, particularly pulmonary toxicity.


Subject(s)
Amiodarone/adverse effects , Anti-Arrhythmia Agents/adverse effects , Lung Diseases/diagnosis , Lung/drug effects , Mucin-1/blood , Amiodarone/blood , Anti-Arrhythmia Agents/blood , Heart Function Tests , Humans , Pulmonary Gas Exchange
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