Systematic review of fungi, their diversity and role in ecosystem services from the Far Eastern Himalayan Landscape (FHL).

Fungi are morphologically and ecologically diverse kingdom but less explored in the global perspective. This systematic review of mainly higher fungi (mushrooms) and lichenized fungi (lichens) was aimed to convey comprehensive knowledge on these understudied taxa, especially considering diversity, research trends, taxonomic/geographic knowledge gaps, and their contribution to ecosystem services. We investigated literature from the Far Eastern Himalayas and adjacent areas. We followed the PRISM (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) framework for the evidence synthesis and reporting. Search strings were used to explore literature both in English and Chinese databases. Publications were validated examining the title, locality, abstract and full text. We included 75 eligible studies after screening 12,872 publications. The result on species diversity extrapolated from literature was consolidated as a species checklist and published on the Global Biodiversity Information Facility (GBIF) portal. This review demonstrates a significant shortage of research work on fungi, and a lack of quantitative data on diversity, ecology, and ecosystem services. Mycological inventories with multidisciplinary perspectives are urgent in the Landscape to better understand the importance of fungi in conservation and sustainable development science. This review is especially useful when global environmental and climate concerns are focused on the use of nature-based solutions, and fungi as integral part of all ecological processes, could play important role in enhancing ecosystem services and therefore benefits coming to people as natural solutions.

Indigenous knowledge and use of lichens by the lichenophilic communities of the Nepal Himalaya.

BACKGROUND: The aim of the study was to document the prevailing indigenous knowledge and various uses of lichens among the lichenophilic communities in the hills and mountainous settlements of Nepal. METHODS: Ethnic uses were recorded during twelve field trips, each of roughly 15 days in three consecutive years, through direct questionnaires administered to 190 respondents. Lichen samples were identified applying microscopic observation and thin layer chromatography (TLC). Voucher specimens of identified species are deposited at TUCH (Tribhuvan University Central Herbarium) in Nepal. RESULTS: Lichens are being used in several ways by different communities of Nepal. We recorded the ethnic use of seven species of lichens belonging to four families (Parmeliaceae, Physciaceae, Ramalinaceae and Usneaceae) and six genera (Heterodermia, Everniastrum, Parmotrema, Ramalina, Thamnolia and Usnea) among the Limbu, Sherpa, Lama, Gurung, Rai, Dalit, Tamang, Chhetri and Brahman communities. The present study revealed six use values namely; Medicinal value (MV), food value (FV), ritual and spiritual value (RSV), aesthetic and decorative value (ADV), bedding value (BV) and ethno-veterinary value (EVV) from different parts of Nepal. Three lichen species, Everniastrum cirrhatum, E. nepalense and Parmotrema cetratum were consumed by the Limbu and Rai communities. The Limbu and Sherpa ethnic groups are regarded as most lichenophilic communities while respondents from Brahman, Chhetri and Tamang communities showed less interest in lichen uses. CONCLUSIONS: The present study contributes to document traditional knowledge on various uses of lichens among nine communities with three different cultural background, inhabitants of eight different altitudinal levels of Nepal. Regarding the six values as identified from this research, significant difference (p = <0.05) were found along altitudinal gradients or locations of the settlements, cultural groups and ethnicity of the respondents.

Seasonal Changes in Bird Species and Feeding Guilds along Elevational Gradients of the Central Himalayas, Nepal.

The Himalayas are a global hotspot for bird diversity with a large number of threatened species, but little is known about seasonal changes in bird communities along elevational gradients in this region. We studied the seasonality of bird diversity in six valleys of the Central Himalayas, Nepal. Using 318 plots with a 50 m radius, located from 2200 to 3800 m a.s.l., and repeated sampling during different seasons (mainly pre-monsoon, monsoon, and post-monsoon), we analyzed 3642 occurrences of 178 species. Birds classified in the literature as resident were more species-rich than migratory birds (140 vs. 38 species). In all six valleys and within the studied elevation range, species richness of all birds showed a peak at mid-elevation levels of 2600 or 3000 m a.s.l. Similar patterns were found for the most species-rich feeding guilds of insectivores (96 species) and omnivores (24 species), whereas the species richness of herbivores (37 species including frugivores) increased towards higher elevations. Among these feeding guilds, only species richness of insectivores showed pronounced seasonal changes with higher species numbers during post-monsoon season. Similarly, individual bird species showed distinct spatio-temporal distribution patterns, with transitions from species dominated by elevational differences to those characterized by strong seasonal changes. In an era of climate change, the results demonstrate that individual bird species as well as feeding guilds might greatly differ in their responses to climate warming and changes in the seasonality of the precipitation regime, two aspects of climate change which should not be analyzed independently.

Macrofungal diversity in community-managed sal (Shorea robusta) forests in central Nepal.

Macrofungi constitute a group of the high-value forest resources worldwide. In this paper, we report species richness and composition of the macrofungi in sal (Shorea robusta) forests of mid-hill central Nepal, which were managed for 4-29 years by the local communities. The sal forests were rich in macrofungi (115 species) with Polyporaceae being the largest family followed by Clavariaceae. Saprotrophic fungi were more common than mycorrhizal species. The proportion of mycorrhiza was <40% of the total macrofungi species which might have indicated the deteriorated condition of the forests before the initiation of conservation management. However, the proportion of mycorrhizal species was slightly higher in the forests managed for >10 years than in the forests managed for short period. The species richness increased with increasing canopy and litter cover. Since silvicultural activities and resource utilization often have negative impacts to macrofungal diversity, these activities need to be optimized to keep balance between forest management and biodiversity conservation.

Characterization of microsatellite loci in the Himalayan lichen fungus Lobaria pindarensis (Lobariaceae).

PREMISE OF THE STUDY: Microsatellite loci were developed for the rare, Himalayan, endemic haploid lichen fungus, Lobaria pindarensis, to study its population subdivision and the species' response to forest disturbance and fragmentation. • METHODS AND RESULTS: We developed 18 polymorphic microsatellite markers using 454 pyrosequencing data and assessed them in 109 individuals. The number of alleles per locus ranged from three to 11 with an average of 6.9. Nei's unbiased gene diversity, averaged over loci, ranged from 0.514 to 0.685 in the three populations studied. The cross-amplification success with related species (L. chinensis, L. gyrophorica, L. isidiophora, L. orientalis, L. pulmonaria, L. spathulata, and Lobaria sp.) was generally high and decreased with decreasing relationship to L. pindarensis. • CONCLUSIONS: The new markers will allow the study of genetic diversity and differentiation within L. pindarensis across its distribution. Moreover, they will enable us to study the effects of forest management on the genetic population structure of this tree-colonizing lichen and to carry out population genetic studies of related species in East Asia.

Molecular phylogenetic identification of Fasciola flukes in Nepal.

Eighty-one Fasciola flukes collected from 8 districts in Nepal were analyzed for their species identification on the basis of their spermatogenic status and nuclear ribosomal internal transcribed spacer 1 (ITS1) and for their phylogenetic relation with Fasciola flukes from other Asian countries on the basis of the mitochondrial NADH dehydrogenase subunit 1 (nad1) gene. Sixty-one flukes (75.3%) were aspermic Fasciola sp., and 20 flukes (24.7%) were identified as Fasciola gigantica. All of the aspermic flukes displayed the Fh/Fg type in ITS1, which was predominant in aspermic Fasciola sp. from China, and most (60 flukes) displayed the Fsp-ND1-N1 haplotype in the nad1, which had an identical nucleotide sequence to the major haplotype (Fg-C2) of the aspermic flukes from China. These results suggest that aspermic Fasciola sp. was introduced into Nepal from China. Furthermore, the results of the diversity indices, neutrality indices, and median-joining network analysis with reference haplotypes from Asian countries suggest that aspermic Fasciola sp. rapidly expanded its distribution. In contrasts, F. gigantica displayed 10 nad1 haplotypes, which showed higher population diversity indices than the haplotypes of aspermic flukes, indicating that the F. gigantica population was clearly distributed in Nepal earlier than the aspermic Fasciola population. Although the F. gigantica haplotypes from Nepal formed a star-like phylogeny consisting of a main founder haplotype (Fg-ND1-N1), together with some F. gigantica haplotypes from Myanmar and Thailand, the Nepal population differed genetically from F. gigantica populations of neighboring countries as each country had distinct founder haplotype(s).