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1.
Cancer Res ; 54(19): 5064-70, 1994 Oct 01.
Article in English | MEDLINE | ID: mdl-7923118

ABSTRACT

Chinese hamster ovary (and many rodent cell lines) transiently delay mitosis and progress into a second cell cycle without undergoing cytokinesis when treated with Colcemid, whereas HeLaS3 (and most human cell lines) arrest permanently in mitosis. We have discussed these differences and their consequences for cell survival under cell cycle-perturbing conditions within the context of mitotic checkpoint control (Schimke et al., Cold Spring Harbor Symp. Quant. Biol., 56: 417-425, 1991). Here, we report studies with mouse BALB/3T3 cell populations which, by the criterion of response to Colcemid, constitute a heterogeneous population with respect to mitotic checkpoint control. Clonal and subclonal populations retain population heterogeneity but with a bias for enrichment of cell populations that respond as do HeLaS3 cells. We have analyzed clones for their propensity for gene amplification as assessed by a stepwise increment selection protocol in methotrexate and report that there are significant differences in amplification propensities that correlate with differences in mitotic checkpoint control properties.


Subject(s)
Gene Amplification , Mitosis , 3T3 Cells , Animals , Cell Line, Transformed , Drug Resistance , Methotrexate/pharmacology , Mice , Tetrahydrofolate Dehydrogenase/genetics
3.
Plant Cell Rep ; 7(7): 535-7, 1988 Dec.
Article in English | MEDLINE | ID: mdl-24240410

ABSTRACT

In vitro culture of pineapple (Ananas comosus) was studied to determine the efficiency of axillary bud culture for rapid propagation of several cultivars. The technique used maximizes the success rate of various steps in the production of pineapple plants. Rapid mass multiplication of plantlets started 9 months after explanting with a significant log phase. The number of plantlets obtained from the culture of a single bud by the thirteenth month ranged from 210 to 380 for 'Perolera'; 300 to 350 for 'PR-1-67'; and 40 to 85 for 'Smooth Cayenne'. The method permits culture of a range of pineapple cultivars. Little morphological variation was observed in young regenerated plants.

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