Evaluation of Failure in Single-Piece Implant Systems: A One-Year Follow-Up Study.

Objective: This study's goal was to assess the failure rate and peri-implant complications of single-piece implant systems over the course of a one-year follow-up. Materials and Methods: Patient records were examined retrospectively. 150 single-piece dental implants were analyzed. Clinical results, implant features, and demographic information were gathered. Implant failure, which is characterized as the total loss of osseointegration, served as the key outcome indicator. Patient satisfaction and peri-implant problems were secondary outcomes. Data analysis employed descriptive statistics. Results: During the one-year follow-up period, the failure rate for single-piece implant systems was 6.7%. The two main factors leading to implant failure were found to be poor osseointegration (60%) and biomechanical overload (40%). 20% of the cases had peri-implant problems, such as peri-implantitis. 85% of the panelists felt that single-piece implants had satisfied their patients. Conclusion: A 6.7% failure rate in single-piece implant systems was seen in this one-year follow-up investigation. The major causes of implant failure were found to be poor osseointegration and biomechanical loading. In 20% of the cases, peri-implant problems such as peri-implantitis, were noted. There was great patient satisfaction. These results highlight the significance of regulating occlusal forces, optimizing osseointegration, and applying preventive measures to ensure the long-term viability of single-piece implant systems.

Comparative Evaluation of Different Post and Core Systems in Maxillary Molar Endodontic Treatments: An Original Study.

Background: Maxillary molar endodontic treatments require the right post and core system. This study tested cast metal, glass fiber with composite resin, carbon fiber with composite resin, and zirconia post and core materials. Materials and Methods: Sixty removed human maxillary teeth were randomly divided into four post and core material groups (n = 15 per group). The teeth acquired their post and core system after normal endodontic treatment. Fracture resistance, cyclic fatigue resistance, and microleakage analyses were statistically assessed. Results: The zirconia post and core material had the highest fracture resistance (mean: 900 N), followed by carbon fiber with composite resin, cast metal, and glass fiber with composite resin. Zirconia had the highest mean cycles to failure (120,000), followed by carbon fiber with composite resin (100,000), cast metal (110,000), and glass fiber with composite resin (90,000). Zirconia had the lowest mean microleakage score (1.8), and glass fiber with composite resin, the highest (3.0). Conclusion: The zirconia post and core material outperformed the others in fracture resistance, cyclic fatigue resistance, and sealing. The cast metal has better fracture and cycle fatigue resistance than glass fiber with composite resin. These findings help doctors choose maxillary molar endodontic post and core materials.

Assessment of Polyetheretherketone Crown in Patients: An Original Research.

Introduction: Polyetheretherketone (PEEK) has a long history of usage in implant abutments, but it has not seen much applications in tooth-supported prosthetics. The purpose of this research was to examine the clinical performance of PEEK crowns as well as the level of patient satisfaction with them. The pilot study was designed as an in vivo longitudinal examination. Material and Methods: Twenty patients each received a PEEK crown during the procedure. In total, 22 implants were inserted, with 11 going into the maxilla and 9 into the mandible. One operator was responsible for carrying out each phase of the procedure. The resin cement was used to lute the crowns that were produced. Baseline, 3 months, 6 months, and 1 year after the crowns were placed, they were evaluated for various parameters using Modified Ryge's Criteria. These evaluations were performed at intervals of 3 months, 6 months, and 1 year. In addition, a questionnaire was used at the same time to assess the level of satisfaction felt by patients. Fisher's exact test was utilized in the process of analyzing the data that were gathered. Results: According to the findings, 92.5% of the crowns met the modified version of Ryge's criterion for a satisfactory rating. Three crowns had a fracture recorded. There was some chipping off observed in five of the crowns. There was not any significant difference in the other factors that were examined. The periodontal health of these three patients showed a slight degree of variance (P = 0.02). Conclusion: The capacity of PEEK crowns to create high-quality prostheses that were deemed satisfactory with a reasonably low rate of fracture during the relative mean duration of 1 year was established by the application of Modified Ryge's Criteria. The PEEK crowns demonstrated their ability to produce quality prostheses.

Comparison of the Photofunctionalization and Platelet-Rich Plasma in the Immediate Implants.

Introduction: Many adjuvant methods have been researched to enhance the immediate implant success in the recent times. The study's goal was to compare the aesthetic and biological results of immediate dental implants in the aesthetic zone to standard tapered root form implants that had not undergone pre-treatment with platelet-rich plasma or photofunctionalization. Materials and Methods: The study used a randomized controlled trial as its design. Ninety subjects were chosen at random and placed into three groups: a control group, a case group, and a group that needed replacement of their maxillary anterior teeth right away following extraction. The interventional groups received the "Platelet-Rich Plasma (PRP)" or "Photofunctionalization (PF)" group before the implant placement. After the delayed loading approach, the prosthesis was given after 6 months. At the second and fourth weeks, as well as 2, 4, 6, and 12 months (P 0.05), follow-up was conducted. The success and survival rate, aesthetic, and biological outcomes were assessed. One-way ANOVA was used to compare outcomes, and repeated-measures ANOVA was used to assess intragroup variations over baseline and follow-up. Results: The distal, mesial, and mean marginal bone loss as well as the aesthetic scores for pink and white did not differ significantly between groups. The implant stability however was significantly higher in the PF and PRP groups as compared to the controls. A single implant failed in all the three groups. The success percentage was similar for all the groups. Conclusion: The stability of immediate dental implants pre-treated with PRP or PF differed statistically significantly than the subjects in the control group, while other parameters remained the similar between the groups.

A Lab-Based Study to Assess the Surface Roughness of Various Investments: An Original Research.

Introduction: Surface roughness of the crowns is dependent on the pattern material used for the making as well as the procedure of investing. The study was aimed to examine the impact of various pattern materials and investment procedures on the surface roughness of nickel-chromium alloy raw castings. Materials and Methods: An in-vitro study was piloted at a tertiary care hospital. Eighty samples of inlay wax and pattern resin were divided equally. They were invested in phosphate-bonded investment material and kept under normal atmospheric pressure as well as invested under a pressure of 3 bars. The surface roughness was calculated with a Profilometer. The values obtained were compared using statistical tools keeping P < 0.05 as significant. Results: Lowest surface roughness was seen for the wax pattern invested under positive pressure. While the highest was seen for the resin patterns invested at room pressure. A significant variance between the wax 1 and wax 2 (P < 0.01); as well as between the wax 2 vs. Resin 2 specimens (P < 0.01) was noted. Conclusions: Wax patterns can be suggested as the material and method of choice because they showed the least amount of surface roughness when placed under pressure. Resin patterns when invested under pressure can also be suggested as an alternate as they also exhibited similar surface roughness as that of the wax.

Shear Strength of Repaired 3D-Printed and Milled Provisional Materials Using Different Resin Materials with and without Chemical and Mechanical Surface Treatment.

The aim of this study was to assess the shear bond strength of 3D-printed and milled provisional restorations using various resin materials and surface finishes. There were 160 preliminary samples in all, and they were split into two groups: the milled group and the 3D-printed group. Based on the resin used for repair (composite or polymethylmethacrylate (PMMA)) and the type of surface treatment utilized (chemical or mechanical), each group was further divided into subgroups. The specimens were subjected to thermocycling from 5 °C to 55 °C for up to 5000 thermal cycles with a dwell time of 30 s. The mechanical qualities of the repaired material underwent testing for shear bond strength (SBS). To identify the significant differences between the groups and subgroups, a statistical analysis was carried out. Three-way ANOVA was used to analyze the effects of each independent component (the material and the bonding condition), as well as the interaction between the independent factors on shear bond strength. Tukey multiple post-hoc tests were used to compare the mean results for each material under various bonding circumstances. The shear bond strengths of the various groups and subgroups differed significantly (p < 0.05). When compared to the milled group, the 3D-printed group had a much greater mean shear bond strength. When compared to PMMA repair, the composite resin material showed a noticeably greater shear bond strength. In terms of surface treatments, the samples with mechanical and chemical surface treatments had stronger shear bonds than those that had not received any. The results of this study demonstrate the effect of the fabrication method, resin type, and surface treatment on the shear bond strength of restored provisional restorations. Particularly when made using composite material and given surface treatments, 3D-printed provisional restorations showed exceptional mechanical qualities. These results can help dentists choose the best fabrication methods, resin materials, and surface treatments through which to increase the durability and bond strength of temporary prosthesis.

Assessment of the Effect of Phototherapy and PRP on the Immediate Implants: An Original Research.

Immediate implants have been gaining popularity in recent times. There are many adjuvant techniques that are followed before implant placement, which have recently shown hopeful results. For this reason, the current comparative study analyzes the effectiveness of platelet-rich plasma (PRP) and photofunctionalization (PF) at a tertiary care dental hospital. In the current clinical trial, 30 subjects were grouped equally to receive immediate dental implants, with one group in which pretreatment of the extraction site was done with the auto-PRP and PF, while the control group received no pretreatment. For 12 months after the placement of the implant, the subjects were observed for alterations in the parameters. The variables analyzed were survival, esthetics, biological integration, and finally the success of the implants in each group. Values were noted and compared using the appropriate statistical tools, while keeping the level of significance at P < 0.05. For the variables of esthetics and survival, no substantial differences were observed in the PRP and PF groups. There was no difference between these experimental groups and the control group. However, there were significant differences for the biological variable as there was more marginal bone loss in the experimental groups. On the whole, the success was greater for the experimental groups than the control group, though not significant. The pretreatment protocols using PRP and PF have shown to improve some characteristics of the immediate implants in the anterior regions. However, further studies are suggested.

Comparison of the Quality of Life Among the Immediate Loading Implant Overdentures Using the Single and the Double Implants: An Original Study.

Introduction: In edentulous individuals, single implant-retained mandibular overdentures are an effective substitute for double implant-retained overdentures. In this current research, the oral health-related quality of life (OHRQoL) was assessed for the immediate loading overdenture using the implants. The comparisons are drawn between the single and double implants. Materials and Methods: Forty subjects received single and double implants for the placement of the mandibular overdentures. Within a week days of implant insertion, they were loaded with overdentures. Using the Oral Health Impact Profile-14 (OHIP-14) questionnaire, the OHRQoL was measured before treatment, one month following treatment, and 12 months thereafter. Results: In both the single implant and double implant groups, participants' mean OHIP-14 scores decreased statistically significantly for the different timelines. For the single implant group, the baseline total mean OHIP-14 score was 14.33, and for double implants, it was 19.15. Following therapy, it decreased to 8.55 in the single implant group and 9.48 for the double implant group at one month, and at 12 months, it further decreased to 3.25 in the single implant group while it was 5.15 in the double implant group. Conclusions: Single and double implants increase the QoL for older subjects who are edentulous at the 1-month and 1-year recall points. When two implants are used by older individuals, the single implant might offer an equivalent quality of life.

Clinical Effectiveness of 3D-Milled and 3D-Printed Zirconia Prosthesis-A Systematic Review and Meta-Analysis.

BACKGROUND: Additive manufacturing (three-dimensional (3D) printing) has become a leading manufacturing technique in dentistry due to its various advantages. However, its potential applications for dental ceramics are still being explored. Zirconia, among ceramics, has increasing popularity and applications in dentistry mostly due to its excellent properties. Although subtractive manufacturing (3D milling) is considered the most advanced technology for the fabrication of zirconia restorations, certain disadvantages are associated with it. METHODS: A systematic review was piloted to compare the clinical performance of zirconium crowns that were fabricated using three-dimensional (3D) milling and 3D printing. A meta-analysis was performed, and studies published up to November 2022 were identified. The terms searched were "Zirconium crowns", "3D printing", "CAD/CAM" (Computer-Aided Design and Computer-Aided Manufacturing), "Milling", "dental crowns", and "3D milling". The characteristics that were compared were the year in which the study was published, study design, age of the patient, country, the number of crowns, the type of crown fabrication, marginal integrity, caries status, and outcomes. Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines were used to structure this systematic review. Out of eleven hundred and fifty titles identified after a primary search, nine articles were included in the quantitative analysis. The research question based on PICO/PECO (Participant, Intervention/exposure, Comparison, and Outcome) was "Do 3D-printed and milled (P) zirconia crowns and FDPs (I) have a better survival rate (O) when conventional prosthesis is also an option (C)"? The data collected were tabulated and compared, and the risk of bias and meta-analysis were later performed. Only nine articles (clinical research) were selected for the study. Since there were no clinical studies on the 3D printing of zirconium crowns, six in vitro studies were considered for the comparison. Zirconium crowns in the milling group had an average minimum follow-up of 6 months. RESULTS: A moderate risk of bias was found, and survival was significant. A high heterogeneity level was noted among the studies. Marginal integrity, periodontal status, and survival rate were high. Linear regression depicted no statistical correlation between the type of cement used and the survival rate. CONCLUSIONS: It can be concluded that the milled crowns had a higher performance and satisfactory clinical survival.

Effects of Blood Contamination and Decontamination Protocol on Reverse Torque Value of Abutment Screws in Dental Implants: An In Vitro Study.

Background and Objective: Loosening of abutment screws in dental implants is a mechanical complication that affects prosthetic treatments and hence, patient satisfaction. Blood contamination of abutment screws may play a role in this phenomenon. However, only limited research attention has been given to this issue. In the present study, we determined the effect of blood contamination and decontamination protocol on the reverse torque value (RTV) of abutment screws. Materials and Methods: A questionnaire-based survey was sent to 210 implantologists requesting feedback on their attitude to the blood contamination issue and the decontamination protocols used. The survey responses were used in a selection of the decontamination solutions that were used in the subsequent in vitro study on the effects of blood decontamination protocol on the RTV of abutment screws. Thus, three study groups were used (n = 20 abutment screws in each group): Group 1 (control group; blood-contaminated screws); Group 2 (screws decontaminated with 5.25% sodium hypochlorite (NaOCl) solution); and Group 3 (screws decontaminated with normal saline solution (0.9%)). Then, each of the connections were subjected to thermocycling, and RTVs of the screw were measured using a digital torque meter. Intragroup and intergroup RTVs were analyzed for significance using analysis of variance (ANOVA) and Tukey's honestly significant difference (HSD) tests. Results: 48% of the implantologists responded to the survey; 80% of them were concerned with blood contamination in the implant connection, especially before abutment loading and 85% of them used either chlorhexidine solution or normal saline solution as the decontamination agent. The mean RTV for Group 2 screws (30.27 ± 2.8 N.cm) was significantly greater than that for Group 3 screws (26.02 ± 1.99 N.cm) which, in turn, was significantly greater than that for Group 1 screws (23.64 ± 1.84 N.cm). Conclusion: Decontamination of blood-covered connections using 5.25% NaOCl solution or normal saline solution restores the RTV of abutment screws. This finding may have clinical relevance in that the decontaminated screws may contribute to the low incidence of screw loosening and, ultimately, improved patient satisfaction.

Radiographic Evaluation of the Gap between Cemented Post and Remaining Gutta-Percha in Endodontically Treated Teeth Performed by Undergraduate Students: A Retrospective Cross-Sectional Study.

The coronal seal in root canal-treated teeth may be compromised depending on the accuracy of post space preparation and post cementation along with remaining gutta-percha. Root canal treatment can be compromised by endotoxins released by the coronal bacteria as a result of microleakage. The study was conducted by undergraduate students to measure the gap between the cemented post and residual gutta-percha. In total, 217 endodontically treated teeth were evaluated with intraoral peri-apical radiographs. Based on the intraoral periapical radiographic examination in the CS-R4 program, Group I had no gap, Group II had a gap of >0 to 2 mm, and Group III had a gap of more than 2 mm between the end of the cemented post and the remaining gutta-percha. In total, 40% (n = 87) of the teeth had no gap, 59% (127) had a gap of >0 to 2 mm, and 1% (n = 3) had a gap of more than 2 mm between the cemented post end and remaining gutta. Chi square test revealed a significant difference in the gap between the post and remaining gutta-percha between males and females students (p < 0.001). In terms of the gap between the cemented post end and the residual gutta-percha, the root canal treated teeth with post and core by undergraduates were clinically acceptable.

The Effect of a Digital Manufacturing Technique, Preparation Taper, and Finish Line Design on the Marginal Fit of Temporary Molar Crowns: An In-Vitro Study.

The aim of this study is to investigate the combined effect of a digital manufacturing technique (subtractive vs. additive), preparation taper (10° vs. 20° TOC), and finish line (chamfer vs. shoulder) on the marginal adaptation of temporary crowns following cementation with a compatible temporary cement. Four mandibular first molar typodont teeth were prepared for full coverage crowns with standard 4 mm preparation height as follows: 10° TOC with the chamfer finish line, 10° TOC with the shoulder finish line, 20° TOC with the chamfer finish line and 20° TOC with the shoulder finish line. Each of the four preparation designs were subdivided into two subgroups to receive CAD/CAM milled and 3D-printed crowns (n = 10). A total of 80 temporary crowns (40 CAD/CAM milled and 40 3D-printed) were cemented to their respective die using clear temporary recement in the standard cementation technique. The samples were examined under a stereomicroscope at ×100 magnification following calibration. Linear measurements were performed at seven equidistant points on each axial surface and five equidistant points on each proximal surface. One-way ANOVA analysis and Tukey HSD (Honestly Significance Difference) were performed. The best marginal fit was seen in group 8, while the poorest fit was noted in group 2. Shoulder finish lines and 10° TOC resulted in higher marginal gaps, especially in CAD/CAM milled group. The selection of 3D-printed crowns may provide a better marginal fit within the range of clinical acceptability. Marginal gaps were within clinical acceptability (50 and 120 µm) in all groups except group 2.

Effect of non-surgical periodontal therapy with adjunct photodynamic therapy on periodontal and glycemic statuses in prediabetic patients with periodontal disease.

OBJECTIVE: Studies have shown that non-surgical periodontal therapy (NSPT) helps reduce periodontal inflammation and glycemic levels in patients with type-2 diabetes mellitus; however, the role of NSPT with adjunct photodynamic therapy on glycemic status and periodontal parameters in prediabetic patients remains unclear. The aim of the present study was to assess the effect of NSPT with and without adjunct PDT on periodontal and glycemic statuses in prediabetic patients with periodontal disease. METHODS: Prediabetic (Group-1) and non-diabetic (Group-2) patients diagnosed with periodontal inflammation were included. In both groups, patients were randomly assigned to test and control-groups. In the test- and control groups, patients underwent NSPT with and without adjunct PDT, respectively. The following parameters were assessed at baseline and at four weeks' follow-up: plaque index (PI); gingival index (GI), probing depth (PD), clinical attachment loss (CAL) and hemoglobin A1c (HbA1c). Collection of demographic data and assessment of full mouth marginal bone loss was performed at baseline. Level of significance was set at P < 0.05. RESULTS: At baseline, there was no difference in PI, GI and PD among patients with and without prediabetes. At 3-weeks' follow up there was no difference in PI, GI and PD among patients without prediabetes. PI (P < 0.01), GI (P < 0.01) and PD (P < 0.01) were significantly higher in the control-group compared with the test-group at 3-weeks' follow-up in patients without prediabetes. The HbA1c levels were significantly higher in patients with prediabetes throughout the study duration compared with individuals without prediabetes. CONCLUSION: One session of NSPT with or without PDT reduce periodontal inflammation but do not influence glycemic levels in prediabetic patients.

Levels of peri-implant sulcular fluid levels of soluble urokinase plasminogen activator receptor and TNF-α among cigarette smokers and non-smokers with peri-implantitis.

BACKGROUND: Soluble urokinase plasminogen activator receptor (suPAR) and tumor necrosis factor-alpha (TNF-α) are inflammatory biomarkers. No studies have yet assessed the suPAR levels in relation with TNF-α in the peri-implant sulcular fluid (PISF) among cigarette smokers and non-smokers with peri-implantitis. OBJECTIVE: The aim was to evaluate PISF levels of suPAR, and TNF-α among cigarette smokers and non-smokers with and without peri-implantitis. METHODS: Sixty male patients with peri-implantitis were included. There were 20 cigarette smokers and 20 and non-smokers with peri-implantitis (Groups 1 and 2), and 20 non-smokers without peri-implantitis (Group 3). Demographic data and information related to cigarette smoking was recorded. Peri-implant clinicoradiographic parameters (plaque index [PI], gingival index [GI], probing depth [PD] and crestal bone loss [CBL]) were assessed. The PISF samples were collected and levels of suPAR and TNF-α were measured. Sample-size estimation was performed and all parameters were statistically assessed. Level of significance was set at P< 0.05. RESULTS: Sixty individuals were included in Groups 1, 2 and 3 (20 in each). Peri-implant PI (P< 0.01), PD (P< 0.01) and mesial (P< 0.01) and distal (P< 0.01) CBL were significantly higher in Group 1 than in Groups 2 and 3. The PISF volume (P< 0.01) and suPAR (P< 0.01) and TNF-α levels (P< 0.01) were significantly higher in Groups 1 and 2 than in Group 3. There was no difference in PISF volume and suPAR and TNF-α levels between patients in Groups 1 and 2. In Group 2, there was a statistically significant correlation between peri-implant PD and PISF suPAR and TNF-α levels (P< 0.01). The suPAR and TNF-α levels are expressed in high concentrations in the PISF of smokers and non-smokers with peri-implantitis compared with non-smokers without peri-implantitis. CONCLUSION: In non-smokers, PISF suPAR and TNF-α levels are correlated with peri-implant PD.

Surface Characteristics of Different Zirconia Dental Implants: A Comparative Study.

Introduction: The invention of dental implants has replaced the fixed partial dentures for the replacement of the teeth. Hence, in the present study, we compared the surface characteristics of various zirconia dental implant systems. Materials and Methods: We conducted an in vitro comparative study of 50 implants of the dental implant systems "Straumann pure ceramic implants (SC) and Bredent dent whiteSKY™ (BW)." Scanning electron microscopy (SEM) was used to compare the groups for the surface characters at the different regions of the implant surface. Results: SC showed deep markings on the surface and droplets, and BW showed smoother surface and bigger droplets under the SEM. Conclusion: SC may perform better in the clinical conditions as the surface characters are better than the BW.

Sleep Disorders among Dental Students: An Original Research.

Introduction: With the increased stress among the students, their sleep patterns have been derailed from the routine. Hence, in our study, we aimed to appraise among the dental students the occurrence of sleep disorders. Materials and Methods: We conducted a questionnaire-based survey study among 1115 dental students of 1-4 years. The survey was based on SLEEP-50 that had 50 questions that were given scores from 1 to 4 based on the response. The scores were analyzed for the statistical connotation, keeping P < 0.05 as significant. Results: We observed that 352 students suffered from sleep disorders. The sex ratio of male:female was 25:75. No significant age variation was seen. For the various disorders, insomnia (25%), nightmares (13%), and sleepwalk (8%), circadian rhythm disorders (34%), obstructive sleep apnea (26%), and narcolepsy (30%) were noted with significant variation. The habit of alcohol was seen in 17% of students. Conclusions: The sleep disorders were prevalent among the dental students due to the great amount of stress caused in the study. They should be educated about the stress management methods and importance of sleep.

Assessment of Failure Rate of Fixed Partial Dentures: A Clinical Study.

Introduction: The most common for the replacement for the teeth are the partial dentures. Hence, in our study, we analyzed the failure rates of the fixed partial dentures (FPDs). Materials and Methods: We conducted a retrospective study from the institutional record and analyzed 150 subjects. The various factors for the FPD's failure were noted and analyzed for any statistical significance. Results: We observed a significant difference between the various factors of the failure caries, periodontitis, uneasiness, and pain. Conclusion: The chief cause for the failure of the FPDs was identified to be caries, periodontitis, uneasiness, and pain.

Prevalence of Stress and Coping Disorders among Dental Students: An Original Research.

Introduction: The students pursuing professional studies such as medicine and dentistry have to endure a lot of stress. Hence, we evaluated the prevalence of stress and coping disorders among dental students. Methods: We conducted a questionnaire-based online survey study among 100 dental students of 1-4 years. The survey was based on the "modified General Health Questionnaire (GHQ-12; Goldberg and Williams, 1988)." The scores were analyzed for the statistical connotation, keeping P < 0.05 as significant. Results: We observed that over half of the dental pupils felt stressed. The dental students resorted to "Destructive coping mechanisms." Less than 15% of students sought medical and psychiatric counseling. The mean GHQ was 12 ± 1.7. Conclusions: Psychological strength is a prerequisite for good health and better performance in the studies as well as for the practice. There is an urgent need to focus on the mental health of the students specifically in the medical and dental fields.

Hypoxia, a dynamic tool to amplify the gingival mesenchymal stem cells potential for neurotrophic factor secretion.

Gingival mesenchymal stem cells (GMSCs) have significant regenerative potential. Their potential applications range from the treatment of inflammatory diseases, wound healing, and oral disorders. Preconditioning these stem cells can optimize their biological properties. Hypoxia preconditioning of MSCs improves stem cell properties like proliferation, survival, and differentiation potential. This research explored the possible impact of hypoxia on the pluripotent stem cell properties that GMSCs possess. We evaluated the morphology, stemness, neurotrophic factors, and stemness-related genes. We compared the protein levels of secreted neurotrophic factors between normoxic and hypoxic GMSC-conditioned media (GMSC-CM). Results revealed that hypoxic cultured GMSC's had augmented expression of neurotrophic factors BDNF, GDNF, VEGF, and IGF1 and stemness-related gene NANOG. Hypoxic GMSCs showed decreased expression of the OCT4 gene. In hypoxic GMSC-CM, the neurotrophic factors secretions were significantly higher than normoxic GMSC-CM. Our data demonstrate that culturing of GMSCs in hypoxia enhances the secretion of neurotrophic factors that can lead to neuronal lineage differentiation.