Protective and Curative Effects of Ethanol extract of Tinospora Cordifolia on Gentamicin-induced Nephrotoxicity in Rats.

Gentamicin induced acute tubular necrosis (ATN) in intrinsic variety of acute renal failure (ARF) results from oxidative stress leading to cellular lipid peroxidation. Tinospora cordifolia (Tc), locally named as 'Guluncha' is an herbal plant with medicinal value possess antioxidant property as well as significant scavenging activity in different extracts and easily available in Bangladesh. This prospective study was approved by the Institutional Review Board of Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh. This study was conducted to assess the effect of ethanol extract of Tc on gentamicin induced renal damage in rats in the department of Pharmacology, BSMMU, Dhaka, Bangladesh from January 2014 to June 2014. For this purpose, sixty rats were divided into six equal groups. Gentamicin (80mg/kg/day, 7 days) was administered and nephrotoxicity was evaluated biochemically by estimating elevated levels of serum creatinine and serum urea. Extant of lipid peroxidation was assessed by estimating renal cortical malondialdehyde (MDA) levels. The ethanol extract of Tc (200mg/kg/day) was administered with gentamicin concurrently and also consecutively to detect preventive and curative effects respectively. Statistically significant amelioration in the biochemical parameters both in serum and renal tissue suggested that active compound or compounds extracted from Tinospora cordifolia have both protective and curative effects against nephrotoxicity, though responsible active ingredient, accurate mechanism or safety profile was not confirmed by this study.

Morphological Effect of Ethanol Extract of Tinospora cordifolia on Gentamicin-induced Nephrotoxicity in Rats.

Recent studies indicate that cellular lipid peroxidation by free radical is an underlying mechanism of acute renal failure induced by gentamicin (GM). Tinospora cordifolia (Tc) extract has been reported to have antioxidant and free radical scavenging activities that is why it is used in present study with the expectation to interrupt the toxic free radical chain reaction of lipid peroxidation in the course of gentamicin administration. For this purpose, sixty rats were divided into six equal groups. Gentamicin (80mg/kg/day, 7 days) was administered to produce nephrotoxicity and structural alterations were evidenced from histopathology of renal tissues which indicates the renal injury and dysfunction in rats. The ethanol extract of Tinospora (200mg/kg/day) was administered with gentamicin simultaneously and also sequentially to observe preventive and curative effects respectively. Both the groups recovered from the gentamicin-induced nephrotoxicity, evidenced from improvement of histopathological features, though accurate mechanism and safety profile is not confirmed by this study.