ABSTRACT
There is a clinical need for a synthetic bone graft substitute that can be used at sites of surgical intervention to promote bone regeneration. Poly(vinylphosphonic acid-co-acrylic acid) (PVPA-co-AA) has recently been identified as a potential candidate for use in bone tissue scaffolds. It is hypothesized that PVPA-co-AA can bind to divalent calcium ions on bone mineral surfaces to control matrix mineralization and promote bone formation. In this study, hydrogels of PVPA-co-AA have been produced and the effect of copolymer composition on the structure and properties of the gels was investigated. It was found that an increase in VPA content led to the production of hydrogels with high porosities and greater swelling capacities. Consequently, improved cell adhesion and proliferation was observed on these hydrogels, as well as superior cell spreading morphologies. Furthermore, whereas poly(acrylic acid) gels were shown to be relatively brittle, an increase in VPA content created more flexible hydrogels that can be more easily molded into bone defect sites. Therefore, this work demonstrates that the mechanical and cell adhesion properties of PVPA-co-AA hydrogels can be tuned for the specific application by altering the copolymer composition. © 2017 The Authors Journal of Biomedical Materials Research Part A Published by Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 255-264, 2018.
Subject(s)
Acrylates/pharmacology , Cell Adhesion/drug effects , Cell Proliferation/drug effects , Hydrogels/pharmacology , Osteoblasts/drug effects , Acrylates/chemical synthesis , Acrylates/chemistry , Biocompatible Materials/chemistry , Biocompatible Materials/pharmacology , Cell Line, Tumor , Cell Survival/drug effects , Humans , Hydrogels/chemical synthesis , Hydrogels/chemistry , Materials Testing , Porosity , Tissue Engineering , WettabilityABSTRACT
There is a clear clinical need for a bioactive bone graft substitute. Poly(vinyl phosphonic acid-co-acrylic acid) (PVPA-co-AA) has been identified as a promising candidate for bone regeneration but there is little evidence to show its direct osteogenic effect on progenitor or mature cells. In this study mature osteoblast-like cells (SaOS-2) and human bone marrow-derived mesenchymal stem cells (hBM-MSCs) were cultured with PVPA-co-AA polymers with different VPA:AA ratio and at different concentrations in vitro. We are the first to report the direct osteogenic effect of PVPA-co-AA polymer on bone cells and, more importantly, this effect was dependent on VPA:AA ratio and concentration. Under the optimized conditions, PVPA-co-AA polymer not only has an osteoconductive effect, enhancing SaOS-2 cell mineralization, but also has an osteoinductive effect to promote hBM-MSCs' osteogenic differentiation. Notably, the same PVPA-co-AA polymer at different concentrations could lead to differential osteogenic effects on both SaOS-2 and hBM-MSCs in vitro. This study furthers knowledge of the PVPA-co-AA polymer in osteogenic studies, which is critical when utilizing the PVPA-co-AA polymer for the design of novel bioactive polymeric tissue engineering scaffolds for future clinical applications. © 2017 The Authors Journal of Biomedical Materials Research Part A Published by Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 168-179, 2018.
