Biochemical and histopathological evaluations of ghrelin effects following cadmium toxicity in the rat testis.

Numerous reports demonstrate that cadmium (Cd) induces oxidative stress by increasing lipid peroxidation and altering antioxidative enzymes status. Thirty male rats were subdivided into control-saline, Cd-saline and Cd-ghrelin groups. A single dose of Cd was injected to induce testicular injury and also ghrelin for 10 consecutive days to group 3. SOD activity decreased and lipid peroxidation increased by Cd administration. The mean activities of GPx and CAT as well as GSH content were lower in the Cd-saline rats; however, they did not statistically differ compared with the controls. Exposure to Cd resulted in complete degeneration of seminiferous tubules with severe depletion of germ cells and arrest in spermatogenesis. Notably, ghrelin treatment not only prevented reduction in SOD, GPx, CAT and GSH level, but also increased enzyme activities form their normal values. Moreover, TBARS concentration was significantly reduced by ghrelin administration. Furthermore, ghrelin pre-treatment resulted in partial but not significant prevention in testicular histopathological features damaged by Cd. In conclusion, the obtained results indicate for the first time the novel evidences of ghrelin ability in promotion of antioxidant enzyme activities and reduction of lipid peroxidation following Cd-induced oxidative stress in the rat testis. These observations also demonstrate that ghrelin may be considered as promising antioxidant agent in prevention and attenuation of testicular injury upon Cd toxicity.

Leucocytozoonosis in domestic birds in southwestern iran: an ultrastructural study.

BACKGROUND: Leucocytozoonosis is a disease of birds caused by obligate intracellular protozoa of the genus Leucocytozoon. We determined the prevalence of Leucocytozoon spp. using light and transmission electron microscopy in domestic birds in southwest of Iran. METHODS: A total of 825 blood smears from 275 birds were examined for presence of infection. The structure morphology of Leucocytozoon spp. was studied using light and electron microscopy. RESULTS: Forty-four (16.0%) of the birds were positives for Leucocytozoon. The detected parasite were found in 14 chickens (5.1%), 12 geese (4.3%), 10 ducks (3.6%), and 8 turkeys (2.9%). The majority of the records were from the northeastern regions. CONCLUSION: Leucocytozoonosis are distributed in the Lorestan province bird population and electron microscopy can resolve the problem to distinguish between similar species of Leucocytozoon.

Cerebral neuroblastoma and pituitary adenocarcinoma in two budgerigars (Melopsittacus undulatus).

Case 1: A tumor mass involving the rostral part of left cerebrum was found in a two-year-old female budgerigar (Melopsittacus undulatus) at necropsy. Histologically, the neoplastic cells were arranged in sheets or cords and occasionally showed nest growth patterns. These uniform tumor cells had a little cytoplasm and ovoid or round basophilic nuclei with clearly distinct cytoplasmic membranes. The tumor cells were strong diffusely immunostained with both neuron-specific enolase (NSE) and neurofilament protein and partially for synaptophysin. They lacked chromogranin A, glial fibrillary acidic protein (GFAP), vimentin, S-100, and cytokeratin antigen expression. Moreover, they had no reaction to antibodies against pituitary hormones, such as adrenocorticotrophic hormone (ACTH), growth hormone, and prolactin. The histological and immunohistochemical examination determined the tumor as neuroblastoma. Case 2: An extremely enlarged pituitary mass was found above the sella turcica of a male budgerigar. It was soft and well delineated from the adjacent structures. On histological examination, this tumor consisted of a sheet of large closely packed polyhedral cells that had scant to a large amount of pale to strongly eosinophilic cytoplasm. The pleomorphic nuclei were apparently variable in shape, from small round hyperchromatic to very large vesicular forms. The cell boundaries were not clearly distinct. The multifocal immunolabelling of neoplastic cells for NSE, synaptophysin, GFAP, and ACTH appeared, whereas a few cells reacted with vimentin and S-100 and stained negative for other markers, which were also utilized for case 1. Histological and immunohistochemical findings led to identification of corticotroph adenocarcinoma in the pituitary gland.

Hexamita infection associated with diarrhoea and stunting in native turkey poults.

Hexamita sp. was identified in 8 cases of sixty (13.3%) stunted diarrhoeic 1- 12- week old native turkey poults. Large numbers of the flagellated parasites were found within the dilated crypts of Lieberkuhn of ileum and jejunum. In TEM studies on the parasagittal section of the parasite, elongated flagellated binuclear parasite was found. The morphological characteristics of the seen protozoan are similar with those described for Hexamita meleagridis. The parasite could be considered as an intestinal pathogenic protozoan parasite, causing stunting and diarrhoea in turkeys in Iran.

Intestinal cryptosporidiosis in turkeys in Iran.

Naturally occurring cryptosporidiosis is reported in turkey poults suffering from diarrhoea and unthriftness in Iran. Histological and ultrastructural studies revealed high number of Cryptosporidium developmental stages mainly located in the mid and terminal portions of small intestine of the poults. Other portions of the intestinal tract were less frequently infected. Oocyst shedding was detected only in 29% of the histologically positive birds. Based on host species, clinical signs, pathology and tissue location of the parasites, Cryptosporidium meleagridis was most likely responsible for these infections. This parasite was also reported recently in a child with diarrhoea in Iran.