ABSTRACT
Bioremediation potential of Phanerochaete chrysosporium strains NCIM 1073, NCIM 1106 and NCIM 1197 to decolourise molasses in solid and liquid molasses media was studied. Strains varied in the pattern of molasses decolourisation on solid medium by Giant colony method. Under submerged cultivation conditions, strain NCIM 1073 did not decolourise molasses while, strains NCIM 1106 and NCIM 1197 could decolourise molasses up to 82% and 76%, respectively. Under stationary cultivation conditions, none of the strains could decolourise molasses. This was overcome by increasing the surface area of the culture in flat bottom glass bottles under stationary cultivation conditions. Under submerged cultivation conditions, growth was more or less same in all strains. However, the lignin peroxidase and manganese peroxidase activities were significantly less in the strain NCIM 1073. Under stationary cultivation conditions, none of the strains could produce enzymes lignin peroxidase, manganese peroxidase and laccase. However, all of them could produce lignin peroxidase and manganese peroxidase when cultivated in flat bottom glass bottles under stationary cultivation conditions.
Subject(s)
Biotechnology/methods , Molasses , Phanerochaete/metabolism , Biodegradation, Environmental , Catalysis , Hydrogen-Ion Concentration , Laccase/chemistry , Models, Biological , Peroxidases/chemistry , Time FactorsABSTRACT
A method for the location of proteins/enzymes by polyacrylamide gel electrophoresis using dialyzable low-molecular-weight fluorescent peptide markers is described. The markers prepared by treating the peptic digest of casein with fluorescamine showed several bands on gel electrophoresis which helped in locating the proteins. The located desired protein could subsequently be purified by extraction.
Subject(s)
Aldose-Ketose Isomerases , Carbohydrate Epimerases/isolation & purification , Electrophoresis, Polyacrylamide Gel , Fluorescent Dyes , Serum Albumin, Bovine/isolation & purification , Subtilisins/antagonists & inhibitors , Dialysis , Fluorescamine , Fluorescence , Plant Proteins/isolation & purification , Plants/analysis , Streptomyces/enzymologyABSTRACT
A simple technique is described for easy removal of high concentration polyacrylamide gels after electrophoresis from glass tubes without breaking them.
