ABSTRACT
The effect of subzero temperatures on the electrophoretic pattern of seminal plasma protein of cattle and buffalo was studied. The profiles of the seminal proteins of these two closely related species differed considerably. Cattle had 11 proteins in the anodic system (pH 8.6) and none in the cathodic system (pH 4.3), while buffalo have 19 in the anodic system (pH 8.6) and 2 proteins in the cathodic system (pH 4.3). Freezing of semen at -5 degrees C for 24 h caused aggregation of seminal proteins in both species. A higher aggregation and loss of proteins were observed when freezing was done in liquid nitrogen at -196 degrees C. The effect was more pronounced in buffalo than in cattle. Loss of more seminal plasma proteins due to cryoinjury in buffalo semen may account for its poorer freezability than that of cattle semen.
ABSTRACT
An investigation on the effect of restricted feeding an GnRH treatment using immature rats of both sexes was made to see if the GnRH can produce its desired effect on the reproductive organs through the pituitary gonadal axis irrespective of the age and nutritional status of the animal. It was observed that immature male and female rats responded differently to the treatment of GnRH and responses are to a great extent age-dependent. Restricted feeding in these animals slowed down the growth of the reproductive organs.