ABSTRACT
Several animal species such as cattle, goats, sheep, and water buffalo provide milk for dairy products. We describe a simple procedure for detecting the species origin of milk used for cheese production. DNA was isolated from Italian mozzarella or Greek feta by sequential organic extractions and resin purification. This DNA was analyzed by polymerase chain reaction-restriction fragment length polymorphism as described previously for meat samples. This procedure differentiated mozzarella made from water buffalo milk and from less expensive bovine milk and also feta cheeses made from bovine, ovine, and caprine milk.
Subject(s)
Cheese , Food Contamination , Food Inspection/methods , Milk , Animals , Cattle , Cytochrome b Group/genetics , DNA/analysis , DNA/isolation & purification , Food Handling/standards , Goats , Polymerase Chain Reaction/methods , Polymorphism, Restriction Fragment Length , Sheep , Species SpecificityABSTRACT
Some samples of oil adjuvants used in vaccine formulations are tested for mutagenic activity using the "Salmonella/microsome test" with different concentrations of samples per plate. No positive mutagenic activity has been found in the products experimented in this work.
