ABSTRACT
Most oropharyngeal squamous cell carcinomas (OPSCCs) are human papillomavirus (HPV)-associated, high-risk (HR) cancers that show a better response to chemoradiotherapy and are associated with improved survival. Nucleophosmin (NPM, also called NPM1/B23) is a nucleolar phosphoprotein that plays different roles within the cell, such as ribosomal synthesis, cell cycle regulation, DNA damage repair and centrosome duplication. NPM is also known as an activator of inflammatory pathways. An increase in NPM expression has been observed in vitro in E6/E7 overexpressing cells and is involved in HPV assembly. In this retrospective study, we investigated the relationship between the immunohistochemical (IHC) expression of NPM and HR-HPV viral load, assayed by RNAScope in situ hybridization (ISH), in ten patients with histologically confirmed p16-positive OPSCC. Our findings show that there is a positive correlation between NPM expression and HR-HPV mRNA (Rs = 0.70, p = 0.03), and a linear regression (r2 = 0.55; p = 0.01). These data support the hypothesis that NPM IHC, together with HPV RNAScope, could be used as a predictor of transcriptionally active HPV presence and tumor progression, which is useful for therapy decisions. This study includes a small cohort of patients and, cannot report conclusive findings. Further studies with large series of patients are needed to support our hypothesis.
Subject(s)
Carcinoma, Squamous Cell , Head and Neck Neoplasms , Oncogene Proteins, Viral , Oropharyngeal Neoplasms , Papillomavirus Infections , Humans , Carcinoma, Squamous Cell/pathology , Cyclin-Dependent Kinase Inhibitor p16 , DNA, Viral/genetics , Human Papillomavirus Viruses , Nucleophosmin , Oncogene Proteins, Viral/genetics , Oropharyngeal Neoplasms/pathology , Papillomaviridae/genetics , Retrospective Studies , Squamous Cell Carcinoma of Head and Neck , Viral LoadABSTRACT
L'étude a pour objectif de lancer la réflexion parmi les acteurs (bailleurs de fonds, responsables politiques, associatifsà) de la région sur l'importance de disposer d'outils et dÆanalyses socio-économiques dans les zones d'interventions et de transformer des paramètres économiques à large échelle vers un niveau plus tangible pour les familles et les autorités locales. Ce processus de vulgarisation des effets économiques dus aux catastrophes naturelles peut favoriser une prise de conscience majeure au niveau de base et un engagement majeur dans le domaine de la réduction des risques et des désastres des autorités locales
