Bone level changes around platform switching and platform matching implants: a systematic review with meta-analysis.

The amount of marginal bone loss is considered an important criterion to evaluate the implant therapy outcome and to predict the prognosis of the implant rehabilitation. The purpose of this systematic review was to examine the available literature comparing clinical and radiological outcomes like the implant failure rates and marginal bone loss around platform switching dental implants versus platform matching ones. English randomized controlled human clinical trials, comparing one or more PS groups with one or more PM groups, with at least 12 months of follow-up after loading and 10 implants, providing carefully the number of PS- and PM- implants used as well as implant survival and data concerning bone level changes or marginal bone loss around implants, were included. Fifteen publications, involving a total of 1439 implants and 642 patients, were eligible. More studies showed less mean marginal bone loss around PS implants and none of them showed differences in terms of implant failure rates. This review confirmed a great effectiveness of platform switching technique to prevent marginal bone resorption. Nevertheless, this result should be interpreted cautiously because of the heterogeneity of the included studies.

Comparative analysis of two systems for HCV genotyping.

The HCV genotype can be determined by PCR using nested primers to structural or non-structural HCV regions, followed by hybridization analysis of the amplified products. In this study, two different systems, both based on PCR and hybridization analysis, were used to determine HCV genotype in 32 HCV positive patients at the Clinic of Infectious Diseases, University of Chieti. The main difference between these commercially available systems lies in the different PCR target. Amplification of PCR targets was obtained from all samples. Hybridization analysis gave unequivocal results for all samples with both methods, yielding a 100% rate of genotype determination, with a complete correlation at the genotype level. A lower concordance at subtype level (65% concordance) was found, due only to two types of discrepancies. Both methods proved easy to use in our hands, adding evidence to their potential usefulness and reliability in clinical settings.

Rapid diagnosis of bacterial contamination of blood: comparison of three automated microbial detection systems.

Rapid methods of determining microbial contamination are needed in suspecting contaminated banked blood or other blood products. In this study, we experimented contaminated blood units with 122 strains of bacteria and fungi. After innoculation, a comparison between ESP Blood Culture System (Difco Laboratories Inc., Detroit MI), BacT/Alert (Organon Teknica, Durham, NC) and Bactec 9240 System was made for their efficiency in the detection of microbial contamination. Experimental data showed a diagnostic relevance of these automated systems with no significant differences in time detection of microbial contamination between the three methods under comparison.

Hepatosplenic infection caused by Candida parapsilosis in patients with acute leukemia.

Candida parapsilosis is an exceptional agent in hepatosplenic candidosis. Here we report two cases of hepatosplenic infection caused by Candida parapsilosis in two patients with acute leukaemia. Open liver-spleen biopsy and previously minimal exposure to systemic antifungal drugs led to a cultural diagnosis in both patients. This report confirms the importance of an early diagnosis of these diseases in order to undertake appropriate therapeutic regimens.

Occurrence of bacteremia in hematologic patients.

In the present study we reviewed eighty-six episodes of bacteremia occurred in 60 neutropenic patients and thirty-one episodes occurred in 30 non-neutropenic patients. Twenty-four out of 60 neutropenic patients suffered from multiple episodes of bacteremia, while only one out of 30 non-neutropenic patients presented multiple episodes. In neutropenic patients, 29 episodes of bacteremia were polymicrobial, whereas only one non-neutropenic patient had polymicrobial bacteremia. Intravascular catheters were the most common source of bacteremia (23.2%) in neutropenic patients, as compared with infections of the genito-urinary tract (45.1%) among non-neutropenic patients. In both groups, aerobic gram-positive cocci were the microorganisms most frequently isolated (71.6%). Anaerobic microorganisms showed an higher incidence in polymicrobial episodes than in monomicrobial episodes x 2 = 5.39 p = 0.02 OR = 2.97 95% CI (1.2-7.7).

Detection of bacterial contamination in bone marrow graft.

A rapid method of determining bacterial contamination was used in a series of fifty-five bone marrow harvests (20 allogeneic and 35 autologous transplantations). The microbiological culture assays executed on bone marrow samples soon after the harvest and before the manipulation, showed a positivity only in the autologous bone marrow group. Another positivity was revealed after the freezing process of bone marrows for autologous transplantation. Two samples in the bone marrow group submitted to purging were positive after treatment and freezing process. In this study, the increase of bacterial contamination frequency seemed to be related to extensive handlings, such as pharmacological in vitro purging and freezing procedures.