Subject(s)
Immunosuppressive Agents/isolation & purification , Spleen/immunology , Animals , Cattle , Chromatography , Chromatography, High Pressure Liquid , Chromatography, Thin Layer , Dose-Response Relationship, Immunologic , Hemolytic Plaque Technique , Immunosuppressive Agents/pharmacology , Mice , Molecular WeightABSTRACT
Spermine, spermidine and a purified spleen extract (PSE) have been compared in vivo in these three tests: hemolytic plaque forming capacity in sensitized mice, delayed hypersensitivity reaction and 3H-thymidine incorporation into various tissue cells. The results obtained demonstrated that the immunosuppressive activity of PSE cannot be attributed to those polyamines. Ion exchange analysis of PSE before and after acid hydrolysis confirmed the absence of free and/or bound polyamines in the studied extract.
Subject(s)
Immunosuppression Therapy , Spermidine/immunology , Spermine/immunology , Spleen/immunology , Animals , DNA/biosynthesis , Dermatitis, Contact/immunology , Growth Inhibitors , Hemolytic Plaque Technique , Mice , Mice, Inbred DBA , Thymidine/metabolism , Tissue Extracts/pharmacologyABSTRACT
A low molecular weight immunosuppressive factor FA which is able to reduce the blastic transformation capacity of lymphoid cells from treated mice has been characterized. It was prepared from a bovine spleen acetone powder and found to be associated partly with high molecular weight carriers in the form of an active complex characterized previously as part of a 'lymphoid chalone' fraction. FA may be obtained by selective ultrafiltration of F followed by P-2 Biogel chromatography of the ultrafiltrate. Thymidine, deoxyinosine and deoxycytidine have been identified as the major constituents of FA by mass spectrometry, ultraviolet absorption data and thin layer chromatography. However, none of these nucleotides has the biological activity of FA.
