ABSTRACT
A reversed-phase high-performance liquid chromatography (HPLC) method with diode-array detection for the quantification of several human salivary peptides is described. Sample pretreatment consisted of the acidification of whole saliva by phosphate buffer. This treatment produced precipitation of mucins, alpha-amylases and other high-molecular-mass salivary proteins, simultaneous inhibition of intrinsic protease activities and reduction of sample viscosity. Direct HPLC analysis by diode-array detection of the resulting acidic sample allowed one to quantify histatin 1, histatin 3, histatin 5, statherin, as well as uric acid, in normal subjects. Moreover, the groups of peaks pertaining to proline-rich proteins and cystatins were tentatively identified. The method can be useful in assessing the concentration of salivary peptides from normal subjects and from patients suffering oral and/or periodontal diseases.
Subject(s)
Chromatography, High Pressure Liquid/methods , Phosphopeptides/analysis , Proteins/analysis , Salivary Proteins and Peptides/analysis , Acids , Buffers , Histatins , Humans , Spectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationABSTRACT
The authors in epidemiologic research on dental caries of primary-school children in a village having optimufluorotic water, put in evidence fluorine paradox phenomena founding high percentual of cariopatic subjects.
Subject(s)
Dental Caries/prevention & control , Fluoridation , Adolescent , Child , Dental Caries/epidemiology , Humans , Italy/epidemiologyABSTRACT
The AA. describe a case of bone producing fibroma rising in a young patient after the drawing of a tooth. Histological reports, effected with different methods, confirmed this diagnosis.
Subject(s)
Fibroma/pathology , Maxillary Neoplasms/pathology , Osteoma/pathology , Adult , Female , HumansABSTRACT
The AA. examine 14,987 bioptic investigations comings from Anatomy and Pathologic Histology Institute of Cagliari University relieved during the period 1936-1968 (twenty-eight years). They stop on main data giving prominence to: incidence of stomatologic cases, of benignant and malignant tumours, of inflammatory and precancerous states in the mouth. They moreover describe neoplasia frequency in reference to histologic type, to sex, to age and to localization.
Subject(s)
Mouth Neoplasms/epidemiology , Adult , Aged , Female , Humans , Italy/epidemiology , Male , Middle Aged , Precancerous Conditions/epidemiologyABSTRACT
The AA. report a case treated with cyst-enucleation, apicaectomy and tooth replantation and then prosthetic management, which they control for the last twenty years, and beyond initial expectations, they could establish an astonishing result indeed.
Subject(s)
Apicoectomy , Odontogenic Cysts/surgery , Tooth Replantation/methods , Adult , Drug Combinations/therapeutic use , Eugenol/therapeutic use , Female , Formaldehyde/therapeutic use , Humans , Longitudinal Studies , Zinc Oxide/therapeutic useABSTRACT
The AA., after to have passed in review etiopathogenetic interpretations of odontomas and symptomatology, report 12 cases, from Odontostomatologic Clinic of Cagliari, dwelling on one of them especially, where the two epithelial and connective components, made evident microscopically, let foresee probable reductions either connective eithen epithelial component.
Subject(s)
Jaw Neoplasms/pathology , Odontogenic Tumors/pathology , Odontoma/pathology , Adolescent , Adult , Female , Humans , MaleABSTRACT
The same type of cancer occurring in both husband and wife has been reported in the literature.(1-6) The authors describe their experience in a community hospital on the central western coast of Florida.
