ABSTRACT
Cellular mechanical properties constitute good markers to characterize tumor cells, to study cell population heterogeneity and to highlight the effect of drug treatments. In this work, we describe the fabrication and validation of an integrated optofluidic chip capable of analyzing cellular deformability on the basis of the pressure gradient needed to push a cell through a narrow constriction. We demonstrate the ability of the chip to discriminate between tumorigenic and metastatic breast cancer cells (MCF7 and MDA-MB231) and between human melanoma cells with different metastatic potential (A375P and A375MC2). Moreover, we show that this chip allows highlighting the effect of drugs interfering with microtubule organization (paclitaxel, combretastatin A-4 and nocodazole) on cancer cells, which leads to changes in the pressure-gradient required to push cells through the constriction. Our single-cell microfluidic device for mechanical evaluation is compact and easy to use, allowing for an extensive use in different laboratory environments.
Subject(s)
Antineoplastic Agents/administration & dosage , Biological Assay/instrumentation , Flow Cytometry/instrumentation , Lab-On-A-Chip Devices , Neoplasms, Experimental/drug therapy , Neoplasms, Experimental/secondary , Apoptosis/drug effects , Cell Movement , Cell Separation/instrumentation , Drug Evaluation, Preclinical/instrumentation , Equipment Design , Equipment Failure Analysis , Flow Injection Analysis/instrumentation , Neoplasms, Experimental/pathology , Optical DevicesABSTRACT
We present a novel optofluidic device for real-time sorting on the basis of cell mechanical properties, measured by optical stretching. The whole mechanism, based on optical forces, does not hamper the viability of the tested cells, which can be used for further analysis. The device effectiveness is demonstrated by extracting a sample population enriched with highly metastatic cells from a heterogeneous cell mixture.
