ABSTRACT
A very sensitive electrochemical biosensor to determine totals triglycerides (TGs) in serum samples has been developed. It is based on the electrochemical oxidation of glycerol at glassy carbon electrodes modified with magnetic nanoparticles bonded to lipase enzyme and copper oxide nanoparticles, both supported on a multiwalled carbon nanotubes/pectin dispersion. Glycerol is produced by enzymatic reaction between the TGs present in samples and the lipase immobilized. The quantification of triglycerides was performed by amperometric measurements. The proposed electrochemical biosensor improves the performance of others methods developed for the TGs quantification. The determination of TGs does not need a pretreatment of serum samples. The PLS-1 algorithm was used for the quantification of TGs. According to this algorithm, the of detection and quantification limits were from 3.2â¯×â¯10-3 gâ¯L-1 to 3.6â¯×â¯10-3 gâ¯L-1, and from 9.6â¯×â¯10-3 to 1.1â¯×â¯10-2 gâ¯L-1, respectively. The sensitivity was 1.64â¯×â¯10-6 Aâ¯L g-1. The proposed electrochemical biosensor exhibited a very good performance, a stability of 20 days, very good reproducibility and repeatability, and it is presented as a very good alternative for the determination of TGs in human serum clinical samples.
Subject(s)
Biosensing Techniques/methods , Lipase/metabolism , Nanocomposites/chemistry , Triglycerides/blood , Calibration , Chitosan/chemistry , Copper/chemistry , Electrochemistry , Electrodes , Enzymes, Immobilized/chemistry , Enzymes, Immobilized/metabolism , Humans , Lipase/chemistry , Magnetite Nanoparticles/chemistry , Nanotubes, Carbon/chemistry , Pectins/chemistry , Triglycerides/chemistryABSTRACT
AIM: Atopic dermatitis (AD) is a common, chronic relapsing inflammatory skin disease characterized by dry skin and variable pruritus sometimes associated with allergic disease in other organs as asthma and rhinoconjunctivitis. AD affects deeply the Quality of Life, thus can be extremely disabling and may cause psychological problems for both affected children and their families. METHODS: In order to investigate the estimated prevalence of the disease and the beliefs of the Italian pediatricians, a group of 437 Italian family pediatricians covering a population of almost 380000 children participated in a study based on a questionnaire of 38 items. RESULTS: According to answers of the participants, the incidence of AD has been estimated around 10% of the population and food allergy is believed to be the trigger of the acute phase of the disease in infants. As a second opinion, dermatologists are consulted more frequently than allergologists. CONCLUSION: The use of emollients is advised in general whilst topical corticosteroids treatment is prescribed only in selected cases; more than 50% of pediatricians do not prescribe topical calcineurin inhibitors.
Subject(s)
Attitude of Health Personnel , Dermatitis, Atopic/drug therapy , Child , Dermatitis, Atopic/epidemiology , Female , Hospitals, Pediatric , Humans , Male , Pediatrics , Prevalence , Surveys and QuestionnairesABSTRACT
A statical investigation conducted by the authors in a random population demonstrated the utility of the determination of antitetanic antibodies by means of Tetan test in order to establish the immunological defence level against infection.
Subject(s)
Antibodies, Bacterial/analysis , Tetanus/immunology , Adolescent , Adult , Aged , Bacterial Vaccines/immunology , Female , Hemagglutination Tests , Humans , Immunity , Male , Middle AgedABSTRACT
Authors investigated tetanic immunity in pediatric age by means of titration of antitetanic antibodies in serum.
