ABSTRACT
Delta-5 androgen therapies seem to enhance the sexual response in experimental animal models and in clinical trial. This study analyzed the influence of dehydroepiandrosterone (DHEA) administration on receptive and proceptive components of female rat sexual behavior. Ovariectomized (OVX) adult rats were divided in six groups submitted to the following treatments for 4 weeks: DHEA 0.5 and 5 mg/kg, by oral gavage, alone or in combination with estradiol benzoate 3 µg/rat; EB 3 and 10 µg/rat as control groups. All animals received progesterone (500 µg/rat) 4 h before the behavioral tests. All animals were tested for the following: receptivity and proceptivity weekly for 4 weeks; partner preference and paced mating behavior at the end of the treatments. Oral administration of DHEA at 5 mg/kg in EB primed rats was able to significantly increase proceptive behaviors, already after 1 week of treatment. The increase was more marked after 3 and 4 weeks of treatment. Behavioral changes were associated to modifications of circulating and brain level of allopregnanolone and beta-endorphin, although circulating hormonal levels were within a physiological range. Hormonal treatment using physiological doses of delta-5 androgens (DHEA) positively affects sexual motivation in OVX rats.
Subject(s)
Dehydroepiandrosterone/administration & dosage , Sexual Behavior, Animal/drug effects , Adrenal Glands/metabolism , Animals , Brain/metabolism , Dehydroepiandrosterone/therapeutic use , Estradiol/analogs & derivatives , Estradiol/pharmacology , Estrogens/pharmacology , Female , Gonadal Steroid Hormones/blood , Ovariectomy , Pregnanolone/metabolism , Rats , Rats, Sprague-Dawley , beta-Endorphin/metabolismABSTRACT
AIM OF THE STUDY: To evaluate the influence of Humulus lupulus extract on sexual behavior in female rats. MATERIALS AND METHODS: Ovariectomized rats hormonally primed with estradiol benzoate (1.5 µg/rat) and progesterone (500 µg/rat) were acutely treated by oral gavage with Humulus lupulus extract dosed at 5, 10 and 25mg/kg and then tested for partner preference and sexual receptivity. RESULTS: The administration of Humulus lupulus extract at the highest dose significantly increased the preference for the stimulus male during the partner preference test and the number of proceptive behaviors during the receptivity test, without affecting the lordosis response. CONCLUSIONS: Humulus lupulus extract increased sexual motivation in hormone-primed female rats.
Subject(s)
Humulus , Motivation/drug effects , Phytoestrogens/pharmacology , Plant Extracts/pharmacology , Sexual Behavior, Animal/drug effects , Animals , Estradiol/pharmacology , Female , Humulus/chemistry , Ovariectomy , Phytoestrogens/isolation & purification , Progesterone/pharmacology , Rats , Rats, Sprague-DawleyABSTRACT
A liquid chromatography tandem mass spectrometry (LC/MS/MS) method has been developed for the quantitative analysis of acetylcholine in rat brain dialysates. The separation of acetylcholine (ACh), choline (Ch), acetyl-ß-methylcholine (IS) from endogenous compounds and Ringer's salts was achieved with cation exchange chromatography. Optimization of chromatographic and mass spectrometry parameters were perfomed in order to improve sensitivity of the method. The limit of detection were 0.05 and 3.75 fmol on column with S/N ratio of 3:1 for ACh and Ch, respectively. The limit of quantitation (LOQ) for ACh and Ch measured in Ringer's solution were 0.05 nM (0.25 fmol) and 3.75 nM (18.75 fmol), respectively at S/N ratio of 10:1. Linearity of the method has been evaluated in the concentrations range between 0.05 and 5.00 nM and 3.75 and 200 nM for ACh and Ch respectively. The correlation coefficients were 0.999 and 0.995 for ACh and Ch respectively, indicating very good linearity. The LC/MS/MS method developed has been applied to evaluate the effect of oral administration of 7-chloro-3-methyl-3,4-dihydro-2H-1,2,4-benzothiadiazine 1,1-dioxide (IDRA21), a positive modulators of AMPA receptor, on the release of ACh in the rat prefrontal cortex by microdialysis.
