ABSTRACT
The aim of this study is to determine the choleretic properties of lyophilized aqueous extract of Tinospora bakis roots. For thus, we have used healthy and cholestatic Wistar rats. After administration of the extract (4 mg/100 g of weight) biliary flow, biliary acids flow, biliary glutathione and glucose concentrations were measured by specific methods. Comparative results between treated rats and non treated rats show: For healthy rats: a significant increase of biliary flow, biliary acids flow in the group of treated rats versus non treated healthy rats. There were no significant difference in the glutathione and glucose concentrations. For cholestatic rats: we noted also significant increase of biliary flow, biliary acids flow and glutathione concentration in the treated rats group. These results suggest that Tinospora bakis has a choleretic effect in vivo which depends on biliary acids and/or glutathione concentration.
Subject(s)
Bile Acids and Salts/metabolism , Bile/metabolism , Cholagogues and Choleretics/pharmacology , Cholestasis/drug therapy , Gallbladder/drug effects , Phytotherapy , Plant Extracts/pharmacology , Plants, Medicinal , Animals , Bile/chemistry , Carbon Tetrachloride Poisoning/complications , Cholagogues and Choleretics/therapeutic use , Cholestasis/etiology , Drug Evaluation, Preclinical , Gallbladder/metabolism , Glucose/analysis , Glutathione/analysis , Male , Medicine, African Traditional , Models, Animal , Plant Extracts/therapeutic use , Plant Roots/chemistry , Rats , Rats, Wistar , Secretory Rate/drug effects , WaterABSTRACT
The aim of this study is to appreciate hepatoprotective properties of lyophilized aqueous extract of Tinospora bakis roots. We used hepatocytes isolated from rats and intoxicated by CCl4. Before and after intoxication cells were treated with different concentrations of Tinospora bakis extract. (1 mg to 4 mg/ml). Protection of cells was evaluated by the decrease of intracellular enzymes release (LDH and ASAT). The impact of time on the cytoprotection was also studied. From each rat we isolated 185.4 millions +/- 71.5 hepatocytes. The percent of ASAT and LDH release was significatively decreased when we compared treated and no treated hepatocytes suggesting cells protection. This cytoprotection is dose independent but is more effective for long course treatment. These results show the direct protection of isolated hepatocytes by Tinospora bakis extract.
