Oral hygiene and periodontal condition of 12- and 15-year-old Greek adolescents. Socio-behavioural risk indicators, self- rated oral health and changes in 10 years.

AIM: To investigate oral hygiene and periodontal status of 12- and 15-year-old Greek adolescents in relation to socio- demographic and behavioural indicators and identify national trends. MATERIALS AND METHODS: In a stratified cluster sample of 2,478 participants, the Simplified Debris Index (DI-S) and the Community Periodontal Index (CPI) were calculated. Socio- demographic/behavioural data were collected through structured questionnaires. Multivariate ordinary regression examined the effect of socio-behavioural indicators on oral hygiene and periodontal conditions. RESULTS: 51.7% of the 12-year-olds and 56.9% of the 15- year-olds had fair and good oral hygiene, respectively. Approximately 30% of the adolescents had healthy periodontium. Bleeding on probing was detected in 23.6% and 21.6% of the 12- and the 15-year-olds, accordingly. Calculus was found in 46.2% and 44.3% of the 12- and the 15-year-old groups, correspondingly. The prevalence of periodontal pockets ?4mm was 3.2%. Significantly higher DI-S scores were found among 12-year-olds who were males, had lower educated mothers or did not use dental floss and among adolescents who brushed their teeth

Simultaneous determination of aminoglycosides and colistins in food.

A novel method for the simultaneous identification and quantification of twelve aminoglycosides (AGs) and two colistins in meat and bovine milk has been developed. The analysis was carried out using liquid chromatography coupled to quadrupole-Orbitrap mass spectrometry (LC-Q-Orbitrap). Among the HILIC (Hydrophilic Interaction Liquid Chromatography) stationary phases tested, the bare silica Poroshell 120 provided the best results. The samples were extracted with an aqueous solution followed by an SPE clean up based on the weak cation exchange mechanism. The validation study was performed carrying out 72 experiments per matrix at six different concentrations in a range encompassing the Maximum Residue Limits. The recoveries were from 72 to 87% in meat (except colistins) and from 82 to 96% in milk. Repeatabilities and intra-lab reproducibilities were lower than 10 and 15%, respectively. Limits of detection were lower than or equal to 33 µg kg-1. Finally, test materials containing AGs prepared for interlaboratory studies were successfully analysed.

Effect of fluoride and of calcium sodium phosphosilicate toothpastes on pre-softened dentin demineralization and remineralization in vitro.

OBJECTIVES: The purpose of this in vitro study was to evaluate the effect of toothpastes containing sodium fluoride in different concentrations or a calcium sodium phosphosilicate system on pre-softened dentin demineralization and remineralization. METHODS: During a ten day pH-cycling protocol, pre-softened bovine root dentin slabs were immersed twice daily, after the demineralization periods, for 2min, in the following toothpaste slurries: (a) non-fluoridated (control), (b) 7.5% calcium sodium phosphosilicate, (c) 1450 ppm F, (d) 2800 ppm F and (e) 5000 ppm F. Subsequently, the specimens were subjected to a 15-h acid resistance test. Surface microhardness was assessed initially and during the pH-cycling and the acid resistance test period. Repeated measurements in each group were analyzed through appropriate regression models for longitudinal data. RESULTS: All fluoride groups, during pH cycling, showed significantly less microhardness loss (p < or = 0.010, p < or = 0.002, p < or = 0.002) and subsequently exhibited increased acid resistance (p < or = 0.010, p < or = 0.001, p < or = 0.001) compared to the control. The 5000 ppm and 2800 ppm F toothpastes, inhibited demineralization significantly more effectively than the 1450 ppm F (p < or = 0.001, p < or = 0.030) and the calcium sodium phosphosilicate toothpaste (p < or = 0.001), while no significant differences were found between the two high fluoride groups (p=0.130). The calcium sodium phosphosilicate toothpaste, during pH cycling showed a difference that approached statistical significance compared to control (p=0.079), but its acid resistance behavior was similar to control (p=0.610). CONCLUSIONS: Under these experimental conditions, the high fluoride toothpastes promoted remineralization and inhibited demineralization more effectively, than the 1450 ppm F, the non-fluoridated (control) and the calcium sodium phosphosilicate toothpastes.