ABSTRACT
Phototherapy using coherent light (lasers) and non-coherent light (light-emitting diodes (LEDs)) has been investigated for the purpose of biomodulation in biological tissues. Several effects can be expected, including pain moderation, biostimulation of cellular tropism, anti-inflammatory effects, regular circulatory stimulation, and tissue repair. The aim of this study was to evaluate the effect of LED (λ945 ± 20 nm, 48 mW) therapy on the regeneration process in femoral lesions of rats (Wistar). Seven irradiation sessions were held, with a 48-h interval between sessions. The animals were euthanised 14, 21, and 28 days after surgery. Bone samples were analysed by histomorphometry, micro X-ray fluorescence spectroscopy, scanning electron microscopy, and optical densitometry. The results demonstrated the effective positive influence of low-intensity LED therapy using the near-infrared region on the tissue repair process in diabetic animals, especially in the early stages of repair (14 and 21 days after surgery). It can be concluded that LED therapy positively influences bone formation in the early stages of the bone repair process in non-diabetic and diabetic animals, without causing changes in the optical density and volume of tissue in the final stages. No influence of LED therapy was observed on the percentage of calcium, percentage of phosphorus, Ca/P ratio, or optical mineral density in non-diabetic animals. However, increased mineral concentration was evident in the diabetic animals treated with the LED during the repair process.
Subject(s)
Diabetes Mellitus, Experimental/pathology , Femur/pathology , Femur/radiation effects , Infrared Rays , Phototherapy , Spectrometry, X-Ray Emission , Wound Healing/radiation effects , Animals , Densitometry , Femur/ultrastructure , Male , Rats, WistarABSTRACT
Este estudo avalia a ação da fosfatase alcalina na remodelação óssea visando estabelecer uma relação entre as alterações do nível sérico desta enzima com os resultados da prática clínica da Implantodontia através da discussão dos conceitos fi siológicos e patológicos do metabolismo ósseo relacionados à obtenção e manutenção do processo de osseointegração. Embora a função da atividade da fosfatase alcalina óssea não esteja completamente esclarecida, é fato que essa enzima desempenha um importante papel no processo de mineralização óssea, sendo essencial para formação óssea normal. As elevações no nível sérico da fosfatase alcalina podem ser sinais indicativos de doenças hepáticas ou de alterações fi siológicas e patológicas do metabolismo ósseo relacionadas com o aumento da atividade osteoblástica, constituindo assim, uma importante informação auxiliar que vem a contribuir para a indicação terapêutica adequada de cada paciente.
