ABSTRACT
Acetaminophen (APAP) hepatotoxicity is the leading cause of acute liver failure in the western world. Oridonin (OD), which is the major active ingredient of the traditional Chinese medicine Rabdosia rubescens, reportedly exerts anti-inflammatory and antioxidative effects. Here, we first find that OD protects against APAP-induced hepatotoxicity. The results of hepatic tissue-associated RNA-seq and metabolomics showed that the protective effects of OD were dependent upon urea cycle regulation. And such regulation of OD is gut microbiota partly dependent, as demonstrated by fecal microbiota transplantation (FMT). Furthermore, using 16S rRNA sequencing, we determined that OD significantly enriched intestinal Bacteroides vulgatus, which activated the nuclear factor erythroid 2-related factor 2 (Nrf2) pathway to regulate redox homeostasis against APAP by urea cycle. In conclusion, our study suggests that the Bacteroides vulgatus-urea cycle-Nrf2 axis may be a potential target for reducing APAP-induced liver injury, which is altered by OD.
Subject(s)
Bacteroides/drug effects , Chemical and Drug Induced Liver Injury/prevention & control , Diterpenes, Kaurane/pharmacology , Gastrointestinal Microbiome/drug effects , Liver/drug effects , Urea/metabolism , Acetaminophen , Animals , Bacteroides/genetics , Bacteroides/metabolism , Chemical and Drug Induced Liver Injury/metabolism , Chemical and Drug Induced Liver Injury/microbiology , Chemical and Drug Induced Liver Injury/pathology , Disease Models, Animal , Dysbiosis , Fecal Microbiota Transplantation , Liver/metabolism , Male , Metabolome , Mice, Inbred C57BL , Mice, Knockout , NF-E2-Related Factor 2/genetics , NF-E2-Related Factor 2/metabolismABSTRACT
UNLABELLED: Acne vulgaris, a very common condition among adolescents, can result in psychological distress, including anxiety and depression. The purpose of this study was to assess the prevalence and risk factors of acne vulgaris among Chinese adolescents. In the Zhou Hai district of Guangdong Province, 3163 students 10 to 18 years old were selected from 7 schools. Information was collected using self-administrated questionnaIres and physician examinations. The prevalence of acne vulgaris was calculated from the collected data. Potential risk factors including age, gender, diet, skin type, sleeping habits, and facial make-up use were analyzed using stepwise logistic regression. The results showed a prevalence of acne vuglaris of 53.5% in all adolescents, with 51.3% in males and 58.6% in females. The prevalence of inflammatory acne in males and females combined was 25.8% and of acne scarring 7.1%. Increased age was related to higher prevalence and severity of acne vulgaris: 15.6%, 44.9%, and 70.4% for 10, 13, and 16 year olds, respectively. Acne vulgaris was more prevalent in girls under and boys over 14 years of age. Significant risk factors of acne vuglaris included age, skin type (oily, mixed, or neutral skin in comparison with dry skin), insufficient sleep, and cosmetic make-up use. CONCLUSION: Acne vulgaris is prevalent among Chinese adolescents 10 to 18 years old. Health education to address this condition in Chinese adolescents could have important implications for public health.
