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1.
Small Methods ; : e2400432, 2024 May 20.
Article in English | MEDLINE | ID: mdl-38767183

ABSTRACT

Electrocatalytic carbon dioxide reduction reaction (CO2RR) has emerged as a promising and sustainable approach to cut carbon emissions by converting greenhouse gas CO2 to value-added chemicals and fuels. Metal-organic coordination compounds, especially the copper (Cu)-based coordination compounds, which feature well-defined crystalline structures and designable metal active sites, have attracted much research attention in electrocatalytic CO2RR. Herein, the recent advances of electrochemical CO2RR on pristine Cu-based coordination compounds with different types of Cu active sites are reviewed. First, the general reaction pathways of electrocatalytic CO2RR on Cu-based coordination compounds are briefly introduced. Then the highly efficient conversion of CO2 on various kinds of Cu active sites (e.g., single-Cu site, dimeric-Cu site, multi-Cu site, and heterometallic site) is systematically discussed, along with the corresponding catalytic reaction mechanisms. Finally, some existing challenges and potential opportunities for this research direction are provided to guide the rational design of metal-organic coordination compounds for their practical application in electrochemical CO2RR.

2.
Microbiome ; 11(1): 229, 2023 10 19.
Article in English | MEDLINE | ID: mdl-37858227

ABSTRACT

BACKGROUND: Ruminant livestock production is a considerable source of enteric methane (CH4) emissions. In a previous study, we found that dietary inclusions of Bacillus subtilis (BS) and Macleaya cordata extract (MCE) increased dry matter intake and milk production, while reduced enteric CH4 emission in dairy cows. The objective of this study was to further elucidate the impact of feeding BS and MCE on rumen methanogenesis in dairy cows using rumen metagenomics techniques. RESULTS: Sixty dairy cows were blocked in 20 groups of 3 cows accordingly to their live weight, milk yield, and days in milk, and within each group, the 3 cows were randomly allocated to 1 of 3 treatments: control diet (CON), control diet plus BS (BS), and control diet plus MCE (MCE). After 75 days of feeding experimental diets, 12 cows were selected from each treatment for collection of rumen samples for the metagenomic sequencing. Results showed that BS decreased ruminal acetate and butyrate, while increased propionate concentrations, resulting in decreased acetate:propionate ratio. The metagenomics analysis revealed that MCE reduced relative abundances of Methanobrevibacter wolinii, Methanobrevibacter sp. AbM4, Candidatus Methanomassiliicoccus intestinalis, Methanobrevibacter cuticularis, Methanomicrobium mobile, Methanobacterium formicicum, and Methanobacterium congolense. Both BS and MCE reduced relative abundances of Methanosphaera sp. WGK6 and Methanosphaera stadtmanae. The co-occurrence network analysis of rumen bacteria and archaea revealed that dietary treatments influenced microbial interaction patterns, with BS and MCE cows having more and stronger associations than CON cows. The random forest and heatmaps analysis demonstrated that the Halopenitus persicus was positively correlated with fat- and protein-corrected milk yield; Clostridium sp. CAG 269, Clostridium sp. 27 14, Haloarcula rubripromontorii, and Methanobrevibacter curvatus were negatively correlated with rumen acetate and butyrate concentrations, and acetate:propionate ratio, whereas Selenomonas rumiantium was positively correlated with those variables. CONCLUSIONS: The present results provided new information for mitigation of enteric methane emissions of dairy cows by feeding BS and MCE to influence rumen microbial activities. This fundamental knowledge is essential for developing enteric CH4 reduction strategies to mitigate climate change and reduce dietary energy waste. Video Abstract.


Subject(s)
Lactation , Microbiota , Female , Cattle , Animals , Bacillus subtilis , Rumen/microbiology , Propionates/metabolism , Methane/metabolism , Diet/veterinary , Acetates/metabolism , Butyrates/metabolism , Plant Extracts , Fermentation
3.
Medicine (Baltimore) ; 100(25): e26264, 2021 Jun 25.
Article in English | MEDLINE | ID: mdl-34160390

ABSTRACT

BACKGROUND: Antiphospholipid antibody syndrome (APS) is a systemic, autoimmune, prothrombotic disease characterized by persistent antiphospholipid antibodies, thrombosis, recurrent abortion, complications during pregnancy, and occasionally thrombocytopenia. At present, there is no consensus on the treatment of this disease. Long-term anticoagulation is recommended in most cases in patients with thrombotic APS. This study aimed to evaluate whether aspirin combined with low-molecular-weight heparin (LMWH) can improve the live birth rate in antiphospholipid syndrome and its correlation with D-dimer. METHODS: The data were retrieved from the WanFang Data, CBM, VIP, CNKI, the Cochrane Library, PubMed, EMBASE, OVID, and Web of Science databases. We collected data on randomized controlled trials of aspirin combined with LMWH in the treatment of pregnant women with APS. The "Risk of Bias Assessment" tool and the "Jadad Scale" provided by the Cochrane Collaboration were used to evaluate the risk of bias and quality of the collected literature. The risk ratio (RR) and its 95% confidence interval (CI) were determined using Statase-64 software. RESULTS: In this study, a total of 11 studies were included, comprising a total of 2101 patients. The live birth rate in pregnant women with APS was higher on administration of aspirin combined with LMWH than with aspirin alone (RR = 1.29, 95% CI = 1.22-1.35, P < .001). d-dimer concentration in plasma predicted the live birth rate, which was higher below the baseline than above it (RR = 1.16, 95% CI = 1.09-1.23, P < .001). The subgroup analysis of the live birth rate was carried out based on the course of treatment, and the results were consistent with the overall results. Begg funnel plot test revealed no publication bias. Sensitivity analysis showed that deleting any study did not affect the results. CONCLUSION: Aspirin combined with LMWH for APS may improve live birth rate, and detection of d-dimer levels in APS pregnant women may predict pregnancy complications and guide the use of anticoagulants.


Subject(s)
Abortion, Habitual/prevention & control , Anticoagulants/administration & dosage , Antiphospholipid Syndrome/drug therapy , Pregnancy Complications, Hematologic/drug therapy , Thrombosis/drug therapy , Abortion, Habitual/blood , Abortion, Habitual/immunology , Antibodies, Antiphospholipid/blood , Antibodies, Antiphospholipid/immunology , Antiphospholipid Syndrome/blood , Antiphospholipid Syndrome/complications , Antiphospholipid Syndrome/immunology , Aspirin/administration & dosage , Biomarkers/blood , Birth Rate , Drug Therapy, Combination/methods , Female , Fibrin Fibrinogen Degradation Products/analysis , Heparin, Low-Molecular-Weight/administration & dosage , Humans , Live Birth , Pregnancy , Pregnancy Complications, Hematologic/blood , Pregnancy Complications, Hematologic/diagnosis , Pregnancy Complications, Hematologic/immunology , Prognosis , Randomized Controlled Trials as Topic , Thrombosis/blood , Thrombosis/complications , Thrombosis/immunology , Treatment Outcome
4.
J Anim Physiol Anim Nutr (Berl) ; 104(3): 831-837, 2020 May.
Article in English | MEDLINE | ID: mdl-32166787

ABSTRACT

The requirement of net protein (NP) and metabolizable protein (MP) by Dorper crossbred ewe lambs grown from 35 to 50 kg of body weight (BW) was assessed by comparative slaughter experiment. Thirty-five ewe lambs (33.5 ± 0.6 kg BW) of F1 crosses of Dorper × thin-tailed Han sheep were used: 7 lambs were slaughtered as reference animals at the start of the trial, and the remaining 28 lambs were randomly divided into 4 groups of 7 lambs each. Three of the 4 groups were fed a pelleted mixed diet (concentrate/roughage = 44:56, dry matter basis) for ad libitum intake or 65% or 45% of ad libitum intake, and they were all slaughtered when the lambs that were fed ad libitum reached 50 kg BW. The lambs from the fourth group were also fed ad libitum and slaughtered at 43 kg BW as the intermediate group. The intake of MP by the animals of these 4 groups was estimated, and their total body protein and protein retention were measured. The daily requirements of NP and MP for maintenance were 1.52 and 3.98 g/kg BW0.75 , respectively, with a partial efficiency of MP utilization for maintenance of 0.38. The MP requirement for growth ranged from 77.4 to 124.5 g/day for average daily gains from 100 to 250 g BW, and the partial efficiency of MP utilization for growth was 0.66. The Dorper crossbred ewe lambs required more MP for both maintenance and growth in comparison with the recommendations of the US nutritional system.


Subject(s)
Animal Feed/analysis , Diet/veterinary , Dietary Proteins/administration & dosage , Sheep/physiology , Animal Nutritional Physiological Phenomena , Animals , Female , Nutritional Requirements , Sheep/genetics
5.
Medicine (Baltimore) ; 98(39): e17180, 2019 Sep.
Article in English | MEDLINE | ID: mdl-31574824

ABSTRACT

BACKGROUND: ATPase family, AAA+ domain containing 2 (ATAD2) is also known as AAA+ nuclear coregulator cancer-associated protein or PRO2000. ATAD2 has been reported as a prognostic factor in different cancer types, but the association between ATAD2 high expression and survival is still unclear. Thereby, this meta-analysis was performed to evaluate the prognostic value of ATAD2 high expression in human cancers. METHODS: All of the studies included were retrieved from PubMed, EMBASE, and Cochrane Library electronic databases. The clinical outcomes were evaluated by calculating hazard ratio (HR) with their 95% confidence interval (CI). RESULTS: Thirteen studies including 2689 patients were eligible for this analysis. The pooled results showed that ATAD2 over-expression was significantly associated with shorter overall survival (OS) (HR = 2.32, 95% CI = 1.77-3.02), as well as shorter recurrence-free survival (RFS), disease-free survival (DFS), and disease-specific survival (DSS) (HR = 1.83, 95% CI = 1.51-2.23) among human cancers. Subgroup analyses for OS were implemented in terms of region, tumor type, and sample size and the results were coincident with overall pooled results. Begg funnel plot and Egger test showed the presence of publication bias for OS. Sensitivity analysis indicated that both results were not affected for removing any study. CONCLUSION: ATAD2 would be likely to act as a prognostic biomarker for the patients of different cancer types and provide a guide on clinical treatment. Prospective clinical studies are needed to support these findings.


Subject(s)
ATPases Associated with Diverse Cellular Activities/analysis , Adenosine Triphosphatases/analysis , DNA-Binding Proteins/analysis , Neoplasms/enzymology , Neoplasms/mortality , Disease-Free Survival , Humans , Prognosis , Proportional Hazards Models
6.
Animals (Basel) ; 9(7)2019 Jul 15.
Article in English | MEDLINE | ID: mdl-31311119

ABSTRACT

We investigated the effects of different types of early feeding on rumen fermentation parameters and the bacterial community in calves. Fifty-four Holstein calves were assigned to three treatments and fed whole milk (M), pasteurized waste milk (WM), or milk replacer (MR). Male calves were slaughtered at the age of two months to measure the stomach masses. The female calves were followed for six months to determine the body weight, blood indices, rumen fermentation, and ruminal bacterial community. At the age of two months, the average daily gain was lower, but the concentration of total volatile fatty acids was greater in the MR group. Starter intake and stomach mass were lower, but the isovalerate molar proportion was greater in the WM group. The blood indices and ruminal bacterial community of the WM group differed from those of the other groups. At the age of six months, the ruminal propionate molar proportion was lower, but the ruminal pH and acetate/propionate ratio were greater in the MR group. In conclusion, calves fed WM had different rumen fermentation and bacterial community during the weaning period, whereas feeding MR produced a long-lasting effect on the rumen environment.

7.
Front Microbiol ; 10: 356, 2019.
Article in English | MEDLINE | ID: mdl-30873143

ABSTRACT

The aim of this study was to explore the effects of saponins on the rumen microbiota and the ruminal metabolome. Alfalfa hay (AH) and soybean hulls (SH) were used as fiber sources for the control diets. The AH and SH diets were supplemented with tea saponins resulting in two additional diets named AHS and SHS, respectively. These 4 diets were fed to 24 young male Holstein cattle (n = 6 per diet). After 28 days of feeding, the rumen fluid from these cattle was collected using an oral stomach tube. Illumina MiSeq sequencing and ultrahigh-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS) were used to investigate the changes in the ruminal microbes and their metabolites. The relative abundance of Prevotellaceae_YAB2003 increased, while Ruminococcaceae_NK4A214 and Lachnospiraceae_NK3A20 decreased in SHS and AHS compared to SH and AHS, respectively. Feeding SHS resulted in higher ruminal concentrations of squalene, lanosterol, 3-phenylpropanoic acid, and citrulline compared to SH. The different microbial genes predicted by Tax4Fun were involved in amino sugar and nucleotide sugar metabolism. The pathways of arginine and proline metabolism, purine metabolism, and pyrimidine metabolism were enriched by different metabolites. Moreover, in the SH group, a positive correlation was observed between Prevotella_1 (Bacteroidetes), Prevotellaceae_YAB2003 (Bacteroidetes), and Christensenellaceae_R.7 (Firmicutes), and the metabolites, including citrulline, lanosterol, and squalene. The increased abundances of Prevotella_1, Ruminococcaceae_UCG.002, and Prevotellaceae_YAB2003 might result in increased fiber digestion and nutrient utilization but nutrient digestion was not measured in the current study. In summary, saponins have the ability to modulate the ruminal microbial community and ruminal metabolites and thus affect the rumen environment. However, the response seems to be dependent on the composition of the basal diet. This study provides a comprehensive overview of the microbial and biochemical changes in the rumen of cattle fed saponins.

8.
Medicine (Baltimore) ; 97(43): e12858, 2018 Oct.
Article in English | MEDLINE | ID: mdl-30412078

ABSTRACT

BACKGROUND: Programmed cell death ligand 1 (PD-L1) overexpression has been reported to be associated with poor prognosis in several human cancers. However, studies on the prognostic value of PD-L1 expression in ovarian carcinoma (OC) remain controversial. This meta-analysis aimed to evaluate comprehensively the prognostic value of PD-L1 in OC. METHODS: Electronic databases, including PubMed, EMBASE, and the Cochrane Library, were searched up until March 28, 2018. Hazard ratio (HR), along with 95% confidence interval (CI), was used to analyze the included outcomes. RESULTS: A total of 10 studies with 1179 OC patients were included in this meta-analysis. There was no significant correlation between PD-L1 expression and overall survival (OS) (HR 1.23, 95% CI 0.85-1.79) and progression-free survival (PFS) (HR 0.88, 95% CI 0.52-1.47) of OC patients. However, the subgroup analysis suggested that positive PD-L1 expression was significantly associated with poor OS (HR 1.66, 95% CI 1.08-2.55) and PFS (HR 2.17, 95% CI 1.31-3.61) among OC patients from Asian countries. Increased PD-L1 expression was also a favorable factor for OS (HR 0.73, 95% CI 0.53-0.99) and PFS (HR 0.58, 95% CI 0.45-0.75) in OC patients from non-Asian regions. No evidence of publication bias was detected by the Egger linear regression test and Begg funnel plot. Sensitivity analyses suggested that the results of this meta-analysis were robust. CONCLUSIONS: The results indicated that PD-L1 expression may be a negative predictor for prognosis of OC patients from Asian countries, and a good predictor for favorable prognosis of OC patients from non-Asian countries. PD-L1 expression has potential to be a prognostic biomarker to guide clinicians for the selection of individuals who may get clinical benefit from anti-PD-1/PD-L1 immunotherapy. Prospective clinical studies are needed to support these findings.


Subject(s)
B7-H1 Antigen/metabolism , Carcinoma/mortality , Ovarian Neoplasms/metabolism , Asia/epidemiology , Biomarkers, Tumor/metabolism , Disease-Free Survival , Female , Humans , Immunotherapy/methods , Ovarian Neoplasms/pathology , Prognosis
9.
Appl Psychol Meas ; 42(7): 538-552, 2018 Oct.
Article in English | MEDLINE | ID: mdl-30237645

ABSTRACT

Imposing content constraints is very important in most operational computerized adaptive testing (CAT) programs in educational measurement. Shadow test approach to CAT (Shadow CAT) offers an elegant solution to imposing statistical and nonstatistical constraints by projecting future consequences of item selection. The original form of Shadow CAT presumes fixed test lengths. The goal of the current study was to extend Shadow CAT to tests under variable-length termination conditions and evaluate its performance relative to other content balancing approaches. The study demonstrated the feasibility of constructing Shadow CAT with variable test lengths and in operational CAT programs. The results indicated the superiority of the approach compared with other content balancing methods.

10.
J Colloid Interface Sci ; 522: 272-282, 2018 Jul 15.
Article in English | MEDLINE | ID: mdl-29604446

ABSTRACT

HYPOTHESIS: Our previous studies have shown that the metal nanoparticle/polymer composite structures fabricated at the liquid/liquid interface have good reusability but lower catalytic activity for heterogeneous reactions in aqueous solutions. This should be attributed to the poor water wettability and more compact structure of the polymer matrices. Therefore, it should be possible to improve the catalytic activity through designing and fabricating a porous composite structure with good water wettability. EXPERIMENTS: A modified liquid/liquid interface adsorption and fabrication method was used. An aqueous solution of copper acetate and a chloroform/DMF mixed solution of PS-b-PAA acted as the two phases. Through spontaneous emulsification, self-assembly of the polymer molecules with Cu2+ ions in the droplets, and adsorption of the formed spherical micelles and nanofibers to the planar liquid/liquid interface, a porous composite microstructure was formed. FINDINGS: This structure consisted of nanofiber-connected nanospheres which have a PS core and a PAA corona. Tiny and well-dispersed Cu nanoparticles were embedded in the hydrophilic corona and were adsorbed on the nanofibers surface as well. After physical cross-linking with 1,6-diaminohexane, the composite material exhibited high catalytic activity and good reusability for the reactions in aqueous solutions. For example, the rate constant for the reduction of p-nitroaniline reached 1965 s-1 g-1.

11.
Asian-Australas J Anim Sci ; 31(6): 864-872, 2018 Jun.
Article in English | MEDLINE | ID: mdl-29103278

ABSTRACT

OBJECTIVE: The objective of this study was to determine the effect of weaning age on intake, performance, nutrition metabolism and serum parameters of beef calves. METHODS: Sixty Droughtmaster crossbred calves were assigned to 5 groups with 12 calves in each group. The calves in control group remained with the dams till the 22-week age, while the calves weaned at 28 d (4 wk), 42 d (6 wk), 56 d (8 wk), and 70 d (10 wk) of age were sent to group 4 wk, group 6 wk, group 8 wk, and group 10 wk, respectively, and then were fed on milk replacer till the 22-week age. Feed intake and body weight and size were record and blood metabolites were measured. And 24 calves of them (6 in each group) were picked randomly for digestion and metabolism trail. Feed, feces and urine sample were taken and measured. RESULTS: Dry matter intake of calves in group 4 wk was significantly lower than those in the remaining groups from wk 17 to 22 (p<0.05). Feed efficiency of the calves was higher in groups 4 wk and 6 wk than those in groups 8 wk and 10 wk from 11 to 13 wk (p<0.05), and calves had higher feed efficiency in group 4 wk, group 6 wk, and group 8 wk than those in group 10 wk from wk 14 to wk 22. Calves in group 4 wk and 6 wk had lower body weight than group 8 wk and group 10 wk and control group at 10-week age (p<0.05) and 13-week age (p<0.05), and calves in group 6 wk had no significant difference in body weight with control group, group 8 wk and 10 wk (p>0.05) but was higher than that of group 4 wk (p<0.05). Calves in group 6 wk had higher final body weight and total gain than group 4 wk, but no difference of total gain with that of groups 8 wk, 10 wk, and control group. And weaning calves at 6-week age brought higher feed efficiency and average daily gain from wk 14 to wk 22, and higher dry matter and organic matter digestibility at 21 wk. CONCLUSION: It is concluded that the weaning of calves at 6 weeks of age gave positive results.

12.
Psychometrika ; 82(2): 498-522, 2017 06.
Article in English | MEDLINE | ID: mdl-28290109

ABSTRACT

Parameter recovery and item utilization were investigated for different designs for online test item calibration. The design was adaptive in a double sense: it assumed both adaptive testing of examinees from an operational pool of previously calibrated items and adaptive assignment of field-test items to the examinees. Four criteria of optimality for the assignment of the field-test items were used, each of them based on the information in the posterior distributions of the examinee's ability parameter during adaptive testing as well as the sequentially updated posterior distributions of the field-test item parameters. In addition, different stopping rules based on target values for the posterior standard deviations of the field-test parameters and the size of the calibration sample were used. The impact of each of the criteria and stopping rules on the statistical efficiency of the estimates of the field-test parameters and on the time spent by the items in the calibration procedure was investigated. Recommendations as to the practical use of the designs are given.


Subject(s)
Calibration , Psychometrics , Humans
13.
Behav Res Methods ; 49(2): 502-512, 2017 04.
Article in English | MEDLINE | ID: mdl-26907749

ABSTRACT

In this article, we propose a simplified version of the maximum information per time unit method (MIT; Fan, Wang, Chang, & Douglas, Journal of Educational and Behavioral Statistics 37: 655-670, 2012), or MIT-S, for computerized adaptive testing. Unlike the original MIT method, the proposed MIT-S method does not require fitting a response time model to the individual-level response time data. It is also computationally efficient. The performance of the MIT-S method was compared against that of the maximum information (MI) method in terms of measurement precision, testing time saving, and item pool usage under various item response theory (IRT) models. The results indicated that when the underlying IRT model is the two- or three-parameter logistic model, the MIT-S method maintains measurement precision and saves testing time. It performs similarly to the MI method in exposure control; both result in highly skewed item exposure distributions, due to heavy reliance on the highly discriminating items. If the underlying model is the one-parameter logistic (1PL) model, the MIT-S method maintains the measurement precision and saves a considerable amount of testing time. However, its heavy reliance on time-saving items leads to a highly skewed item exposure distribution. This weakness can be ameliorated by using randomesque exposure control, which successfully balances the item pool usage. Overall, the MIT-S method with randomesque exposure control is recommended for achieving better testing efficiency while maintaining measurement precision and balanced item pool usage when the underlying IRT model is 1PL.


Subject(s)
Academic Performance/statistics & numerical data , Models, Psychological , Reaction Time , Computers , Humans
14.
Anim Sci J ; 88(1): 72-78, 2017 Jan.
Article in English | MEDLINE | ID: mdl-27112278

ABSTRACT

The effects of flavonoids on methanogenesis and microbial flora in Dorper × thin-tailed Han crossbred ewes were evaluated in two experiments. To investigate the effects of flavonoids on nutrient digestibility and nitrogen balance, 18 ewes (60.0 ± 1.73 kg body weight (BW)) were allotted to two dietary treatments in experiment one, a control diet and the control diet supplemented with flavonoids (2 g/head/day). In experiment two, the effects of supplementary flavonoids on ruminal fermentation and microbial flora were investigated using quantitative polymerase chain reaction with six ewes (67.2 ± 0.79 kg BW) with ruminal cannula assigned to the identical dietary treatments used in experiment one. Supplementary flavonoids improved the apparent digestibility of nitrogen (N, P < 0.001) and neutral detergent fibre (NDF, P = 0.024) and decreased daily CH4 output (P < 0.001). The ruminal pH (P = 0.638) and ammonia (P = 0.690) were not affected by supplementary flavonoids, whereas the total volatile fatty acid (VFA) content increased (P = 0.037). Supplementary flavonoids decreased ruminal populations of protozoans (P = 0.002) and methanogens (P < 0.001) and increased the populations of Fibrobacter succinogenes (P = 0.016). In conclusion, flavonoids improved the digestibility of organic matter and reduced CH4 output by inhibiting the populations of microbes involved in methanogenesis.


Subject(s)
Animal Nutritional Physiological Phenomena/drug effects , Diet/veterinary , Dietary Supplements , Flavonoids , Methane/biosynthesis , Morus/chemistry , Rumen/metabolism , Rumen/microbiology , Sheep/metabolism , Sheep/microbiology , Animals , Depression, Chemical , Dietary Fiber , Digestion/drug effects , Fatty Acids, Volatile/metabolism , Female , Fermentation/drug effects , Fibrobacter , Flavonoids/isolation & purification , Flavonoids/pharmacology , Gastrointestinal Microbiome/drug effects , Nitrogen/metabolism , Plant Leaves/chemistry
15.
Asian-Australas J Anim Sci ; 27(2): 161-8, 2014 Feb.
Article in English | MEDLINE | ID: mdl-25049939

ABSTRACT

THIS STUDY AIMED TO INVESTIGATE DIETARY CONCENTRATE: forage ratios (C:F) and undegraded dietary protein (UDP) on nitrogen balance and urinary excretion of purine derivatives (PD) in lambs. Four Dorper×thin-tailed Han crossbred castrated lambs with 62.3±1.9 kg body weight at 10 months of age were randomly assigned to four dietary treatments in a 2×2 factorial arrangement of two levels of C:F (40:60 and 60:40) and two levels of UDP (35% and 50% of CP), according to a complete 4×4 Latin-square design. Each experimental period lasted for 19 d. After a 7-d adaptation period, lambs were moved into individual metabolism crates for 12 d including 7 d of adaption and 5 d of metabolism trial. During the metabolism trial, total urine was collected for 24 h and spot urine samples were also collected at different times. Urinary PD was measured using a colorimetric method and creatinine was measured using an automated analyzer. Intake of dry matter (DM) (p<0.01) and organic matter (OM) (p<0.01) increased as the level of UDP decreased. Fecal N was not affected by dietary treatment (p>0.05) while urinary N increased as the level of UDP decreased (p<0.05), but decreased as dietary C:F increased (p<0.05). Nitrogen retention increased as dietary C:F increased (p<0.05). As dietary C:F increased, urinary excretion of PD increased (p<0.05), but was not affected by dietary UDP (p>0.05) or interaction between dietary treatments (p>0.05). Daily excretion of creatinine was not affected by dietary treatments (p<0.05), with an average value of 0.334±0.005 mmol/kg BW(0.75). A linear correlation was found between total PD excretion and PDC index (R(2) = 0.93). Concentrations of creatinine and PDC index in spot urine were unaffected by sampling time (p>0.05) and a good correlation was found between the PDC index (average value of three times) of spot urine and daily excretion of PD (R(2) = 0.88). These results suggest that for animals fed ad libitum, the PDC index in spot urine is effective to predict daily excretion of PD. In order to improve the accuracy of the spot sampling technique, an appropriate lag phase between the time of feeding and sampling should be determined so that the sampling time can coincide with the peak concentration of PD in the urine.

16.
Yan Ke Xue Bao ; 18(2): 126-8, 2002 Jun.
Article in Chinese | MEDLINE | ID: mdl-15510654

ABSTRACT

PURPOSE: To improve nursing quality after general anesthesia in children with strabismus operation and prevent from accidents. METHODS: 136 children with strabismus operation under general anesthesia were observed and cared by the same nursing staff. Efficient managements were performed to cure postoperative complications. The nursing records were analyzed. RESULTS: In the study, glossocoma occurred in 1 case, asphyxiation in 2 cases, fever in 7 cases, pain in 25 cases, vomiting without eating after awakening in 33 cases. One patient tore off the bandaging lint because of dysphoria. CONCLUSION: A careful preoperative and postoperative observation, early and predictive management of postoperative complications help the children with strabismus operation under general anesthesia to recover and prevent from the accidents.


Subject(s)
Strabismus/nursing , Anesthesia, General , Child , Child, Preschool , Female , Humans , Male , Postoperative Complications/prevention & control , Strabismus/surgery
17.
Yan Ke Xue Bao ; 18(4): 259-60, 2002 Dec.
Article in Chinese | MEDLINE | ID: mdl-15515773

ABSTRACT

PURPOSE: To improve the prevention and nursing of complications after non-penetration trabecullar surgery (NPTS) combined with the implant. METHODS: 25 patients (35 eyes) were observed and nursed after they underwent NPTS combined with reticulated hyaluronic acid implant. RESULTS: In 35 eyes, the flat anterior chamber I occurred in 3 eyes, the flat anterior chamber 11 occurred in 2 eyes, and 2 eyes had hyphema in early period after operation. CONCLUSION: It is important to the success of operation that postoperative complications are detected timely, managed and nursed properly.


Subject(s)
Glaucoma Drainage Implants , Glaucoma/surgery , Postoperative Complications/nursing , Trabeculectomy/nursing , Adolescent , Adult , Aged , Aged, 80 and over , Anterior Chamber/pathology , Female , Glaucoma/nursing , Humans , Hyaluronic Acid/therapeutic use , Hyphema/etiology , Male , Middle Aged
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