ABSTRACT
PURPOSE: Members of the genus Kudoa Meglitsch, 1947 are known to infect the muscles of commercially important fishes worldwide, including those in the order Siluriformes. This paper describes the occurrence of a new species of Kudoa in the catfish Brachyplatystoma rousseauxii based on morphological study and molecular analysis of the ribosomal RNA gene (SSU rDNA). METHODS: Fifteen specimens of Brachyplatystoma rousseauxii were purchased from fishing zones near Mosqueiro Island, Belém, Pará, Brazil. After necropsy, tissue samples and cysts were analyzed using a stereomicroscope, and fresh slides were viewed under a light microscope to confirm parasitic infection. The tissue fragments were removed and processed for molecular and histological analyses. RESULTS: Microscopic pseudocysts were found in the epaxial region of skeletal muscle fibers in 80% of the analyzed specimens. The myxospores were quadrangular with four shell valves (SV), pyriform polar capsules (PC), and internal symmetry. Phylogenetic analyses indicated that the new species formed a cluster with the species previously described in the Amazon, being close to two freshwater species. CONCLUSIONS: Morphological differences and molecular data of SSU rDNA support that Kudoa rousseauxii n. sp. is a new species that infects B. rousseauxii, a freshwater fish with intense migratory cycles that is widely captured and consumed in the Amazon.
Subject(s)
Catfishes , Cnidaria , Fish Diseases , Myxozoa , Parasitic Diseases, Animal , Animals , Catfishes/parasitology , Cnidaria/genetics , DNA, Ribosomal/genetics , Fish Diseases/parasitology , Fresh Water , Muscle, Skeletal/parasitology , Myxozoa/genetics , Parasitic Diseases, Animal/parasitology , Phylogeny , RiversABSTRACT
Objetivou-se verificar a qualidade de ovos brancos de acordo com os aspectos internos e externos comercializados em diferentes estabelecimentos do município de Paragominas - PA. Os ovos foram coletados de aleatoriamente em diferentes pontos comerciais. As análises consideradas no estudo, foi de: integridade e aparência da casca e peso do ovo, altura da câmara de ar, peso, altura e diâmetro da gema para o cálculo de índice da gema, altura e peso do albúmen e cor da gema. Os dados foram submetidos à análise de variância e ao teste de Tukey. Os ovos dos grandes supermercados apresentaram melhores índices de qualidade interna e os piores resultados quanto a peso, trincas visíveis e sujeiras. Os ovos de pequenos supermercados apresentaram o maior índice de trincas não visíveis e os de médio porte os menores índices de sujeiras e trincas.
