Effect of live yeast supplementation on energy partitioning and ruminal fermentation characteristics of steers fed a grower-type diet in heat-stress conditions.

The objective of this trial was to determine the influence of live yeast supplementation (LY), environmental condition (ENV), and their interaction (TRT) on energy partitioning, nitrogen metabolism, and ruminal fermentation dynamics of steers receiving a grower-type diet. The effects of LY and ENV were investigated using a 2 × 2 crossover design that spanned five periods. Eight Angus-crossbred steers were randomly split into pairs and housed in four outdoor pens outfitted with an individualized feeding system. Animals were limit-fed a grower diet (DIET) at 1.2% shrunk body weight (SBW) with no live yeast supplementation (NOY) or a grower diet top-dressed with 10 g LY/d for 14 d (1.2 × 1012 CFU/d). On days 13 and 14, animals were subjected to one of two ENV conditions, thermoneutral (TN; 18.4 ± 1.1 °C, 57.6 ± 2.8% relative humidity [RH]) or heat stress (HS; 33.8 ± 0.6 °C, 55.7 ± 2.7% RH), in two side-by-side, single-stall open-circuit, indirect respiration calorimetry chambers. Data were analyzed using a random coefficients model. Carryover effects were examined and removed from the model if not significant. Gross (GE), digestible, metabolizable, heat, and retained energies were not influenced by DIET, ENV, or TRT (P ≥ 0.202). Gaseous energy, as a percentage of GE, tended to increase during HS (P = 0.097). The only carryover effect in the study was for oxygen consumption (P = 0.031), which could be attributed to the tendency of NOY (P = 0.068) to have greater oxygen consumption. DIET, ENV, or TRT (P ≥ 0.154) had no effects on total animal methane or carbon dioxide emissions. Similarly, DIET, ENV, or TRT (P ≥ 0.157) did not affect ruminal pH, redox, protozoa enumeration, ruminal ammonia concentrations, and acetate-to-propionate ratio. Propionate concentrations were the greatest in animals in TN conditions receiving LY (P = 0.034) compared to the other TRT. This effect is mirrored by TN-LY tending to have greater acetate concentrations (P = 0.076) and total VFA concentrations (P = 0.065). Butyrate concentrations tended to be greater for animals fed LY (P = 0.09). There was a tendency for LY to have elevated numbers of Fusobacterium necrophorum (P = 0.053). Although this study lacked effects of LY on energy partitioning, nitrogen metabolism, and some ruminal parameters during HS, further research should be completed to understand if LY is a plausible mitigation technique to enhance beef animals' performance in tropical and sub-tropical regions of the world.

About 70% of global beef production is located in tropical and sub-tropical regions. With elevated temperatures and significant humidity, these regions impose heat stress on beef animals. Heat stress is the main antagonist to ruminant production as it decreases dry matter intake and digestion and increases energy expenditure due to the animal's need for thermoregulation. Supplementation of live yeast products has proven efficacious at improving ruminal fermentation dynamics. This study sets out to determine if live yeast supplementation to animals in heat stress conditions can positively affect energy partitioning, nitrogen metabolism, and ruminal parameters. Additionally, this study models the ruminal performance after exposure to heat stress or live yeast supplementation. This study identified several interesting in vitro dynamics of previously stressed- or supplemented rumen fluid. Although there were a lack of effects for live yeast supplementation on energy partitioning, nitrogen metabolism, and some ruminal parameters during heat stress, further research should be completed in order to understand if live yeast supplementation is a plausible mitigation technique to enhance the performance of beef animals reared in tropical and sub-tropical regions of the world.

The influence of extended supplementation of quebracho extract to beef steers consuming a hay diet on digestion, ruminal, and blood parameters.

The addition of natural plant secondary compounds to ruminant feed has been extensively studied because of their ability to modify digestive and metabolic functions, resulting in a potential reduction in greenhouse gas emissions, among other benefits. Condensed tannin (CT) supplementation may alter ruminal fermentation and mitigate methane (CH4) emissions. This study's objective was to determine the effect of quebracho CT extract [QT; Schinopsis quebracho-colorado (Schltdl.) F.A. Barkley & T. Meyer] within a roughage-based diet on ruminal digestibility and kinetic parameters by using the in situ and in vitro gas production techniques, in addition to blood urea nitrogen (BUN) and ruminal (volatile fatty acid [VFA], NH3-N, and protozoa count) parameters. Twenty rumen-cannulated steers were randomly assigned to four dietary treatments: QT at 0%, 1%, 2%, and 3% of dry matter (DM; QT0: 0% CT, QT1: 0.70% CT, QT2: 1.41% CT, and QT3: 2.13% CT). The in situ DM digestibility increased linearly (P = 0.048) as QT inclusion increased, whereas in situ neutral detergent fiber digestibility (NDFD) was not altered among treatments (P = 0.980). Neither total VFA concentration nor acetate-to-propionate ratio differed among dietary treatments (P = 0.470 and P = 0.873, respectively). However, QT3 had lower isovalerate and isobutyrate concentrations compared with QT0 (P ≤ 0.025). Ruminal NH3 and BUN tended to decline (P ≤ 0.075) in a linear fashion as QT inclusion increased, suggesting decreased deamination of feed protein. Ruminal protozoa count was reduced in quadratic fashion (P = 0.005) as QT inclusion increased, where QT1 and QT2 were lower compared with QT0 and QT3. Urinary N excretion tended to reduce in a linear fashion (P = 0.080) as QT increased. There was a treatment (TRT) × Day interaction for in vitro total gas production and fractional rate of gas production (P = 0.013 and P = 0.007, respectively), and in vitro NDFD tended to be greater for QT treatments compared with no QT inclusion (P = 0.077). There was a TRT × Day interaction (P = 0.001) on CH4 production, with QT3 having less CH4 production relative to QT0 on day 0 and QT2 on days 7 and 28. Feeding QT up to 3% of the dietary DM in a roughage-based diet did not sacrifice the overall DM digestibility and ruminal parameters over time. Still, it is unclear why QT2 did not follow the same pattern as in vitro gas parameters. Detailed evaluations of amino acid degradation might be required to fully define CT influences on ruminal fermentation parameters and CH4 production.