Mpox Pharyngitis.

A case of mpox pharyngitis in absence of cutaneous lesions is reported. Usually, clinical presentation is either a cutaneous eruption or a combination of cutaneous and mucosal lesions. In patients with atypical pharyngitis, regardless of the presence of skin lesions, pharyngeal swabs should be collected to rule out mpox.

Assessing the role of ulvan as immunonutrient in Solea senegalensis.

Immunonutrition is a promising and viable strategy for the development of prophylactic measures in aquaculture. Ulvan, a sulphated marine polysaccharide from green seaweeds, has many biological activities including the immunomodulatory ones. The aim of this study was to assess the short and long-term effects of an ulvan-rich extract obtained from U. ohnoi as immunonutrient in Senegalese sole juveniles. In this work, an ulvan-rich extract from Ulva ohnoi has been obtained by the hot water method and isolated by ethanol precipitation. The FTIR analysis revealed that the ulvan-rich extact had very similar characteristics to previously published ulvan spectra. The total sulfate and protein content was 24.85 ± 3.98 and 0.91 ± 0.04 %, respectively. In vitro assays performed in Senegalese sole (Solea senegalensis) macrophages showed that the ulvan obtained in this study did not compromise the cell viability at concentrations up to 1 mg ml-1 and expression levels of lyg, irf1, il6, il10, c7, tf and txn were significantly upregulated in a concentration dependent-manner. Finally, S. senegalensis juveniles were fed basal diets and diets supplemented with the ulvan-rich extract at ratios 1 and 2 % for 30 days and then, challenged with Photobacterium damselae subsp. piscicida (Phdp). Thereafter, ulvan was withdrawn from the diet and all juveniles were fed the basal diet for 30 days. At 30 days post withdrawal (dpw), juveniles were challenged with Phdp. The expression profiles of a set of genes related to the immune system in spleen were evaluated as well as the lysozyme, peroxidase and bactericidal activity in plasma. Dietary effects of 1 % ulvan resulted in a boost of the immune response and increased disease resistance at short-term whereas juveniles fed diets supplemented with 2 % ulvan showed a significant decrease in the bactericidal activity and lack of protection against Phdp. At long-term (30 days after the withdrawal of ulvan), an improved response was observed in juveniles previously fed 1 % ulvan.

Anti-Bacterial and Immunostimulatory Properties of Ulvan-Loaded Chitosan Nanoparticles for Use in Aquaculture.

Alternative prophylactic strategies to limit farm animal infection are needed in order to avoid the use of antibiotics. Anti-bacterial and immunostimulatory properties of bioactive compounds are of great interest in aquaculture. Marine derived polysaccharides, such as chitosan and ulvan, together with nanotechnology, have become the focus of attention in the scientific community due to their wide range of biological properties. In this work, chitosan and ulvan-loaded chitosan nanoparticles (referred as CS-TPP NPs and CS-UL-TPP NPs, respectively), obtained by the ionotropic gelation method, had round shape, and the mean sizes were 137.00 ± 5.44 and 325.50 ± 4.95 nm, respectively. No study about the anti-bacterial activity of both types of NPs against Photobacterium damselae subsp. piscicida, an important fish pathogen, has been reported so far. Furthermore, the potential immunostimulatory effects of CS-UL-TPP NPs after oral administration in fish have not yet been evaluated. The percentage of bacterial inhibition against P. damselae subsp. piscicida was determined through in vitro assays, and it was significantly higher in CS-UL-TPP NPs than in CS-TPP NPs at concentrations below 0.03 mg mL-1. The effects on the immune system of CS-TPP and CS-UL-TPP NPs were evaluated in Solea senegalensis juveniles at 30 days after oral administration. Lysozyme activity as well as gene expression levels of il1b, il6, hamp1, tf and c3 was significantly higher in CS-UL-TPP NP-treated groups than in the controls, and no significant differences were observed in CS-TPP NP-treated groups. Thus, ulvan extracted from the macroalgae Ulva ohnoi could improve anti-bacterial and immunostimulant properties of CS-TPP NPs thereby making them suitable to be used as vaccine adjuvant or as immunostimulant.

Labyrinthine Bifurcation of the Facial Nerve.

The labyrinthine bifurcation of the facial nerve is extremely rare. Diverse congenital temporal bone anomalies usually coexist, and a detailed preoperative evaluation is needed to detect them. We report a case of labyrinthine bifurcation of the facial nerve detected on the preoperative evaluation of a patient with congenital aural atresia.

Spatial patterns associated with the distribution of immature stages of Aedes aegypti in three dengue high-risk municipalities of Southwestern Colombia.

Aedes aegypti mosquitoes are the main vector of human arbovirosis in tropical and subtropical areas. Their adaptation to urban and rural environments generates infestations inside households. Therefore, entomological surveillance associated with spatio-temporal analysis is an innovative approach for vector control and dengue management. Here, our main aim was to inspect immature pupal stages in households belonging to municipalities at high risk of dengue in Cauca, Colombia, by implementing entomological indices and relating how they influence adult mosquitos' density. We provide novel data for the geographical distribution of 3,806 immature pupal stages of Ae. aegypti. We also report entomological indices and spatial characterization. Our results suggest that, for Ae. aegypti species, pupal productivity generates high densities of adult mosquitos in neighbouring households, evidencing seasonal behaviour. Our dataset is essential as it provides an innovative strategy for mitigating vector-borne diseases using vector spatial patterns. It also delineates the association between these vector spatial patterns, entomological indicators, and breeding sites in high-risk neighbourhoods.

Daily dataset of precipitation and temperature in the Department of Cauca, Colombia.

This study used the geostatistical Kriging methodology to reduce the spatial scale of a host of daily meteorological variables in the Department of Cauca (Colombia), namely, total precipitation and maximum, minimum, and average temperature. The objective was to supply a high-resolution database from 01/01/2015 to 31/12/2021 in order to support the climate component in a project led by the National Institute of Health (INS) named "Spatial Stratification of dengue based on the identification of risk factors: a pilot study in the Department of Cauca". The scaling process was applied to available databases from satellite information and reanalysis sources, specifically, CHIRPS (Climate Hazards Group InfraRed Precipitation with Station Data), ERA5-Land (European Centre for Medium-Range Weather Forecasts), and MSWX (Multi-Source Weather). The 0.1° resolution offered by both the MSWX and ERA5-Land databases and the 0.05° resolution found in CHIRPS, was successfully reduced to a scale of 0.01° across all variables. Statistical metrics such as Root Mean Squared Error (RMSE), Mean Absolute Error (MAE), Person Correlation Coefficient (r), and Mean Bias Error (MBE) were used to select the database that best estimated each variable. As a result, it was determined that the scaled ERA5-Land database yielded the best performance for precipitation and minimum daily temperature. On the other hand, the scaled MSWX database showed the best behavior for the other two variables of maximum temperature and daily average temperature. Additionally, using the scaled meteorological databases improved the performance of the regression models implemented by the INS for constructing a dengue early warning system.

Craniofacial and dental features in children aged 3-5 years with congenital Zika syndrome.

OBJECTIVE: Zika virus infection has been associated to congenital zika syndrome (CZS) in newborns and is characterized by microcephaly, central/axial motor and sensory dysfunction, dysphagia among other previously described severe health complications. CZS is usually diagnosed postpartum by evident/apparent neural development problems. Although there are some reports of craniofacial/dentition development in CZS, several clinical oral aspects are still unknown. This study describes some structural and functional characteristics of facial and cranial growth and deciduous dentition in CZS-affected children. MATERIAL AND METHODS: Some cranial, facial and dental characteristics were determined in 14 children with CZS aged 3-5 years and compared them against 12 apparently healthy children paired by age and gender. RESULTS: Fourteen CZS cases presented microcephaly, maxillary prognathism, altered facial thirds, asymmetric pupillary line, bruxism (p = 0.006), deep and anterior open bite and distal step decidual molar relationship (p = 0.031). CZS children cannot feed by themselves and most cannot walk and have not develop coordinated and intelligible language according to their chronological age. In contrast, controls presented normal skull features, have autonomous locomotion skills, speak intelligible language, feed by themselves, presented a harmonic intermaxillary relationship and have symmetrical facial thirds. CONCLUSION: Microcephaly, dysphagia, bruxism, mandibular retrognathia, altered facial proportions and malocclusion are the main craniofacial and oral features at CZS. CLINICAL RELEVANCE: The complications of CZS including those related with the face and the oral cavity are still being identified. This study revealed some cranial, facial and oral features in children affected by CSZ. Interdisciplinary rehabilitation protocols must address these syndromic features that could improve children and parents living conditions.

Spatial patterns associated with the distribution of immature stages of Aedes aegypti in three high-risk dengue municipalities of Southwestern Colombia / Patrones espaciales asociados a la distribución de estadios inmaduros de Aedes aegypti en tres municipios de alto riesgo para el departamento del Cauca, Colombia

Aedes aegypti mosquitoes are the main vector of human arbovirosis in tropical and subtropical areas.  Its adaptation to urban and rural environments generates infestations inside households. Therefore, entomological surveillance in association with spatio-temporal analysis is an innovative approach to vector control and dengue management. The main aim was to inspect immature pupal stages in households belonging to municipalities at high risk of dengue in Cauca, Colombia by implementing entomological indices and relating how they influence adult mosquito density. Here, we provide novel data for the geographical distribution of 3,806 immature pupal stages of Ae. aegypti. We also report entomological indices and spatial characterization. The results suggest that for Ae. aegypti species, pupal productivity generates high densities of adults in neighbouring households, evidencing seasonal behaviour. This dataset is of great importance as it provides an innovative strategy for vector-borne disease mitigation using vector spatial patterns and their association with entomological indicators and breeding sites in high-risk neighbourhoods.

Los mosquitos Aedes aegypti son el principal vector de las arbovirosis humanas en zonas tropicales y subtropicales. Su adaptación a entornos urbanos y rurales genera infestaciones en el intradomicilio de las viviendas. De aquí que, la vigilancia entomológica en asociación con el análisis espacial y el análisis espacio-temporal sean un enfoque innovador para el control de vectores y la gestión del dengue.El objetivo principal de la investigación fue realizar una comparación de la vigilancia entomológica, mediante el uso de índices cuantitativos de pupas y de adultos en tres municipios de alto riesgo de dengue Patía (El Bordo), Miranda y Piamonte del departamento del Cauca, con el fin de examinar cómo influye la productividad de pupas, entre índices entomológicos, en la densidad de mosquitos adultos y otros patrones espaciales y temporales. Ae. aegypti , sus índices entomológicos y su caracterización espacial. Los resultados sugieren que, para las especies de Ae. aegypti , la productividad de pupas genera altas densidades de adultos en las viviendas vecinas, evidenciando un comportamiento estacional.Estos resultados son de gran importancia ya que proporciona una estrategia innovadora para la mitigación de enfermedades transmitidas por vectores utilizando patrones espaciales de los vectores y su asociación con indicadores entomológicos y lugares de cría en barrios de alto riesgo para la transmisión del dengue.

Spatial Analysis of Dengue Clusters at Department, Municipality and Local Scales in the Southwest of Colombia, 2014-2019.

Dengue is an arbovirus transmitted by mosquitoes of the genus Aedes and is one of the 15 main public health problems in the world, including Colombia. Where limited financial resources create a problem for management, there is a need for the department to prioritize target areas for public health implementation. This study focuses on a spatio-temporal analysis to determine the targeted area to manage the public health problems related to dengue cases. To this end, three phases at three different scales were carried out. First, for the departmental scale, four risk clusters were identified in Cauca (RR ≥ 1.49) using the Poisson model, and three clusters were identified through Getis-Ord Gi* hotspots analysis; among them, Patía municipality presented significantly high incidence rates in the time window (2014-2018). Second, on the municipality scale, altitude and minimum temperature were observed to be more relevant than precipitation; considering posterior means, no spatial autocorrelation for the Markov Chain Monte Carlo was found (Moran test ˂ 1.0), and convergence was reached for b1-b105 with 20,000 iterations. Finally, on the local scale, a clustered pattern was observed for dengue cases distribution (nearest neighbour index, NNI = 0.202819) and the accumulated number of pupae (G = 0.70007). Two neighbourhoods showed higher concentrations of both epidemiological and entomological hotspots. In conclusion, the municipality of Patía is in an operational scenario of a high transmission of dengue.

Holistic understanding of the response of grapevines to foliar application of seaweed extracts.

Viticulture is highly dependent on phytochemicals to maintain good vineyard health. However, to reduce their accumulation in the environment, green regulations are driving the development of eco-friendly strategies. In this respect, seaweeds have proven to be one of the marine resources with the highest potential as plant protective agents, representing an environmentally-friendly alternative approach for sustainable wine production. The current work follows an interdisciplinary framework to evaluate the capacity of Ulva ohnoi and Rugulopteryx okamurae seaweeds to induce defense mechanisms in grapevine plants. To our knowledge, this is the first study to evaluate Rugulopteryx okamurae as a biostimulator . This macroalgae is relevant since it is an invasive species on the Atlantic and Mediterranean coast causing incalculable economic and environmental burdens. Four extracts (UL1, UL2, RU1 and RU2 developed from Ulva and Rugulopteryx, respectively) were foliar applied to Tempranillo plants cultivated under greenhouse conditions. UL1 and RU2 stood out for their capacity to induce defense genes, such as a PR10, PAL, STS48 and GST1, mainly 24 hours after the first application. The increased expression level of these genes agreed with i) an increase in trans-piceid and trans-resveratrol content, mainly in the RU2 treated leaves, and, ii) an increase in jasmonic acid and decrease in salicylic acid. Moreover, an induction of the activity of the antioxidant enzymes was observed at the end of the experiment, with an increase in superoxide dismutase and catalase in the RU2-treated leaves in particular. Interestingly, while foliar fungal diversity was not influenced by the treatments, alga extract amendment modified fungal composition, RU2 application enriching the content of various groups known for their biocontrol activity. Overall, the results evidenced the capacity of Rugulopteryx okamurae for grapevine biostimulation, inducing the activation of several secondary metabolite pathways and promoting the abundance of beneficial microbiota involved in grapevine protection. While further studies are needed to unravel the bioactive compound(s) involved, including conducting field experiments etc., the current findings are the first steps towards the inclusion of Rugulopteryx okamurae in a circular scheme that would reduce its accumulation on the coast and benefit the viticulture sector at the same time.

Dizziness Evaluation and Characterisation of Patients with Posterior Circulation Stroke in the Emergency Department; a Case Series Study.

Introduction: Dizziness is a common scenario in the Emergency Departments (EDs). Among dizziness underlying causes, the posterior circulation stroke is especially relevant due to its mobimortality and concerning misdiagnosis rates. Therefore, we conducted this study to assess dizziness evaluation and baseline characteristics of patients with PS in the ED. Methods: We conducted a 3-year retrospective observational study on PS cases confirmed by magnetic resonance imaging (MRI). Concretely, we analysed the demographic profile of these patients, the initial PS clinical presentation, and diagnostic workup (with emphasis on dizziness evaluation) performed at the ED. Results: During the study period, 85 cases were registered. Risk factors for cardiovascular disease were present in 85.5% and previous visits to the ED due to dizziness were recorded in 16.5%. The main clinical presentation was dizziness, concretely as an acute vestibular syndrome (38.8%) with additional neurological signs or symptoms (80%). Evaluation by the otolaryngologist on call was requested in less than 10% of the cases and included the HINTS protocol use with a sensitivity of 100% for central nervous system underlying causality. A brain CT study was always performed with a sensitivity of 27%. However, 96.47% of patients were primarily admitted to the Neurology hospitalization ward and MRI was always performed in a mean time of 3.21 days confirming the diagnosis. Conclusions: Dizziness is the most frequent symptom of PS. Patients usually present an AVS (associated with additional N-SS or not) and HINTS bedside examination is the most adequate protocol to differentiate a PS from other AVS causes until the diagnostic confirmation via MRI. Interestingly, mainly otolaryngologists seem to use HINTS. However, the use of CT is widespread despite its poor value.

Aging envisage imbalance of the periodontium: A keystone in oral disease and systemic health.

Aging is a gradual and progressive deterioration of integrity across multiple organ systems that negatively affects gingival wound healing. The cellular responses associated with wound healing, such as collagen synthesis, cell migration, proliferation, and collagen contraction, have been shown to be lower in gingival fibroblasts (the most abundant cells from the connective gingival tissue) in aged donors than young donors. Cellular senescence is one of the hallmarks of aging, which is characterized by the acquisition of a senescence-associated secretory phenotype that is characterized by the release of pro-inflammatory cytokines, chemokines, growth factors, and proteases which have been implicated in the recruitment of immune cells such as neutrophils, T cells and monocytes. Moreover, during aging, macrophages show altered acquisition of functional phenotypes in response to the tissue microenvironment. Thus, inflammatory and resolution macrophage-mediated processes are impaired, impacting the progression of periodontal disease. Interestingly, salivary antimicrobial peptides, such as histatins, which are involved in various functions, such as antifungal, bactericidal, enamel-protecting, angiogenesis, and re-epithelization, have been shown to fluctuate with aging. Several studies have associated the presence of Porphyromonas gingivalis, a key pathogen related to periodontitis and apical periodontitis, with the progression of Alzheimer's disease, as well as gut, esophageal, and gastric cancers. Moreover, herpes simplex virus types 1 and 2 have been associated with the severity of periodontal disease, cardiovascular complications, and nervous system-related pathologies. This review encompasses the effects of aging on periodontal tissues, how P. gingivalis and HSV infections could favor periodontitis and their relationship with other pathologies.

La prevención de la fiebre del dengue, reto y desafío en la Atención Primaria de Salud / The prevention of dengue fever: a challenge in primary health care

RESUMEN Es indudable que, entre las arbovirosis, la fiebre del dengue constituye un grave problema de salud, por lo que se hace necesario incentivar a la población en aras de solucionar las dificultades que impiden la labor de prevención de esta enfermedad y el establecimiento de estrategias comunitarias con acciones educativas y de comunicación social por parte del médico y de la enfermera en la Atención Primaria de Salud, que permita consolidar el trabajo interdisciplinario e intersectorial en el enfrentamiento al vector y esta enfermedad como su consecuencia. El objetivo de la investigación es destacar la importancia del trabajo de prevención de esta enfermedad como reto y desafío en la Atención Primaria de Salud.

ABSTRACT There is no doubt that, among the arboviruses, dengue fever constitutes a serious health problem, for which it is necessary to encourage the population in order to solve the difficulties that impede the work of preventing this disease and the establishment of community strategies with educational and social communication actions by the doctor and the nurse in primary health care, which allows the consolidation of interdisciplinary and intersectoral work in confronting the vector and this disease as its consequence. The objective of the research is to highlight the importance of the work of prevention of this disease as a challenge in primary health care.

Relación entre fuerza de prensión manual, función física y riesgo de caídas en personas mayores / Relationship between handgrip strength, physical function, and risk of falls in older people

RESUMEN Introducción: La fuerza de prensión manual permite evaluar debilidad muscular en personas mayores y presenta correlación con la fuerza general y masa muscular, por lo que podría ayudar en la detección precoz del deterioro de la función física y riesgo de caídas. Objetivo: Relacionar la fuerza de prensión manual con la función física y riesgo de caídas en personas mayores. Métodos: Estudio descriptivo, correlacional. Evaluó 87 personas mayores (24 hombres y 63 mujeres) entre 65 y 75 años, de 4 organizaciones sociales de Talca, Chile, seleccionados por un muestreo no probabilístico por conveniencia. Para medir la fuerza de prensión manual se utilizó́ un dinamómetro de mano, para valorar la función física se usó la prueba Short Physical Performance Battery; con las pruebas de estación unipodal y time up and go se evaluó riesgo de caídas. Resultados: Se observaron relaciones significativas moderadas entre la fuerza de prensión manual y el test Short Physical Performance Battery (p= 0,001; r= 0,473) y con todas las pruebas que componen esta batería. Se evidenció una relación entre la fuerza de prensión y la prueba de estación unipodal (p= 0,001; r= 0,472), mientras que la relación con la prueba time up and go fue inversa débil (p= 0,002; r= -0,398). Conclusión: Existe relación entre la fuerza de prensión manual con la función física y el riesgo de caídas personas mayores. Este hallazgo sugiere que la fuerza de prensión manual podría usarse en la práctica clínica como instrumento de detección precoz de la pérdida de la función física y riesgo de caídas.

ABSTRACT Introduction: Handgrip strength is a way to evaluate muscle weakness in older people and it has been seen to show a high correlation with general strength and body muscle mass, which is why it could help in the early detection of deterioration in physical function. loss of lower limb strength and risk of falls. Objective: To relate handgrip strength with physical function and risk of falls in older people. Methods: Descriptive, correlational study. It evaluated 87 older people (24 men and 63 women) between 65 and 75 years old, from 4 social organizations in the city of Talca, Chile, selected through a non-probabilistic convenience sampling. To measure the handgrip strength, a hand dynamometer was used, to assess physical function the Short Physical Performance Battery test was used, and the unipodal station and time up and go tests were used to evaluate balance static and dynamic respectively. Results: Moderately significant relationships were observed between the handgrip strength in the Short Physical Performance Battery test (p = 0.001; r = 0.473) and with all the tests that make up this battery. A moderate one was also evidenced between the grip strength and the unipodal station test (p = 0.001; r= 0.472), while the relationship with the time up and go test was weak inverse (p = 0.002; r = -0.398). Conclusion: There is a relationship between handgrip strength with physical function and the risk of falls in older people. This finding is important because it suggests that handgrip strength could be used in clinical practice as an indirect indicator or as an early detection tool for loss of general strength capacity, physical fitness, and risk of falls.

Measurement of stigma and associated characteristics in people with tuberculosis in Medellín, Colombia: a cross-sectional study.

BACKGROUND: Stigma towards tuberculosis (TB) delays diagnosis and compromises adherence to treatment. We measured the degree of stigma and identified the sociodemographic and clinical characteristics that were associated with a higher degree of stigma in patients with pulmonary and extrapulmonary TB in Colombia. METHODS: We conducted a cross-sectional study with 232 participants included in the TB control program in 2017. Sociodemographic and clinical variables were measured. The stigma component was measured through a validated scale and a multiple linear regression was used. RESULTS: The study analysed 232 patients, of which 52.2% were men, 53.5% were between 27 and 59 y of age and 66.8% had a basic-medium education level. Two characteristics were significantly related to a higher stigma score: the basic-medium education level and homeless status. Homeless status increased the stigma score by 0.27. In contrast, the adjusted stigma score decreased by 0.07 if the patient's health status was perceived as 'healthy'. CONCLUSION: Stigma is maximized in homeless patients and patients with a low education level. It is minimized in patients who perceive their state of health as 'healthy'.

Arbovirus vectors in municipalities with a high risk of dengue in Cauca, Southwestern Colombia.

The Culicidae family has two of the most important disease vector genus: Aedes spp. and Culex spp. Both of these are involved in the transmission of arboviruses. Here, we provide novel data for the geographical distribution of 2,383 specimens in the Culicidae family. We also report the percentage of houses infested with these vectors, and Shannon and Simpson diversity indices in three municipalities located in Cauca, Colombia. This dataset is relevant for research on vector-borne diseases because Aedes spp. can transmit arboviruses such as dengue, Zika and chikungunya, and Culex spp. is a well-known vector of West Nile virus and Venezuelan equine encephalitis.

Competencias investigativas en profesionales de Enfermería de la Atención Primaria de Salud: necesidad inaplazable / Researching competences in Nursing professionals of Primary Health Care: urgent need

RESUMEN La enfermería es una profesión que debe investigar distintas y mejores formas de brindar cuidados basados en el avance de los conocimientos y resultados obtenidos mediante la investigación. Esta profesión tiene dos compromisos: responder a las demandas de salud de una sociedad dinámica donde los conocimientos científicos y tecnológicos se hacen obsoletos con gran rapidez; y como disciplina, generar conocimientos, divulgarlos y aplicarlos para mejorar el cuidado a las personas y grupos sociales; sin embargo, no todos sus profesionales están conscientes de la necesidad de mantener una búsqueda activa y permanente a través de la superación profesional, por lo que estos autores destacan la importancia de desarrollar sus competencias investigativas en vínculo, en lo fundamental, con la atención primaria de salud.

ABSTRACT Nursing is a profession that must research on different and better ways of providing care based on the advancement of knowledge and results obtained through research. This profession has two commitments: to respond to the health demands of a dynamic society where scientific and technological knowledge is rapidly becoming obsolete; and as a discipline, generate knowledge, disseminate it and apply it to improve care for people and social groups; however, not all its professionals are aware of the need to maintain an active and permanent search through professional improvement, so these authors highlight the importance of developing their researching competencies in connection, fundamentally, with primary health care.

Evaluation of an oral DNA nanovaccine against photobacteriosis in Solea senegalensis.

Infectious diseases are one of the main causes of social and economical losses in world aquaculture. Senegalese sole (Solea senegalensis) is an important species for aquaculture in southern Europe, whose production is affected by the appearance of bacterial diseases such as photobacteriosis, a septicemia caused by Photobacterium damselae subsp. piscicida (Phdp). The aim of this study was to obtain an oral DNA nanovaccine and to evaluate its efficacy against Phdp in S. senegalensis juveniles. For this purpose, the amplified product corresponding to the protein inosine-5'-monophophate dehydrogenase (IMPDH) from Phdp, was cloned into the expression vector pcDNA™6.2/C-EmGFP-GW obtaining the DNA vaccine named as pPDPimpdh. The correct transcription and protein expression was verified at 48 h post tansfection in HEK293 cells. Chitosan nanoparticles (CS-TPP NPs) were prepared by ionotropic gelation and their features were appropriate for use as oral delivery system. Therefore, pPDPimpdh was protected with chitosan CS-TPP NPs throughout complex coacervation method giving as a result a DNA nanovaccine referred as CS-TPP+pPDPimpdh NPs. Sole juveniles were vaccinated orally with CS-TPP NPs, pPDPimpdh and CS-TPP+pPDPimpdh NPs followed by a challenge with Phdp at 30 days post vaccination (dpv). The relative percentage survival (RPS) for pPDPimpdh vaccinated groups was 6.25%, probably due to its degradation in the digestive tract. RPS value obtained for CS-TPP NPs and CS-TPP+pPDPimpdh NPs was 40% and antibodies were observed in both cases. However, a delay in mortality was observed in sole juveniles vaccinated orally with CS-TPP+pPDPimpdh NPs. In fact, an upregulation of tf, mhcII, cd8a and igm in the posterior gut and c3, hamp1, tf and cd4 in spleen was observed in juveniles vaccinated with CS-TPP+pPDPimpdh NPs. After challenge, a modulation of cd8a and cd4 expression levels in the posterior gut and c3, tf, lyg, cd4, igm and igt expression levels in spleen was observed. Moreover, the concentration of lysozyme in skin mucus significantly increased in fish vaccinated orally with CS-TPP+pPDPimpdh NPs at 11 dpc. These data indicate that oral vaccination with CS-TPP+pPDPimpdh NPs could be acting through the non-specific immune responses as well as the specific humoral and cell mediated immunity and provide the first step toward a development of an oral DNA nanovaccine against Phdp in sole.

Dental, Occlusal, and Craniofacial Features of Children With Microcephaly Due to Congenital Zika Infection: 3 Cases Report From Valle del Cauca, Cali-Colombia-2020.

OBJECTIVE: Describes dental, occlusal, and craniofacial characteristics of 3 children aged 3 to 4 years with microcephaly due to congenital Zika infection in Cali Valle del Cauca, 2020. DESIGN: Three children case report with congenital Zika virus microcephaly. SETTING: Institutional. PATIENTS: Three children with maternal viral infection confirmed by polymerase chain reaction during first trimester of pregnancy were included and were born from 2016 to 2017. INTERVENTIONS: Oral and mouth functional examination was performed including soft tissue examination; lingual and labial frenulum; evaluation of swallowing and chewing; craniofacial analysis; dimension of dental arch; intercanine and intermolar distance, palate form; relationship and growth of maxilla, mandible, and facial dental midline using plaster models; and complementary image analysis. MAIN OUTCOME MEASURES: Child and mother sociodemographic features, craniofacial measurements; dental and oral features; maxillary and mandibular measures; and speech, swallowing, and chewing disorders. RESULTS: Small head circumference at birth and at the time of clinical evaluation was compared to normal children of approximately their age. Upper third of the face was short, and presence of hypertonic masticatory muscles with hypotonic swallowing muscles, dysphagia, dyslalia, bruxism, lip incompetence, tongue interposition, and hypersalivation and epilepsy were the main medical problem. They have complete primary dentition with normal dental morphology, tooth eruption altered, dental caries, and dental malocclusion was identified. CONCLUSION: There are no changes in the dental formula and dental morphology in the deciduous dentition. They present severe chewing and speaking limitation, facial disproportion, and occlusal problems that warrant dental and medical attention.

Solea senegalensis Bacterial Intestinal Microbiota Is Affected by Low Dietary Inclusion of Ulva ohnoi.

The inclusion of macroalgae in the diets of farmed fish offers the opportunity for an added-value dietary ingredient to the nutraceutical feed. The composition of algae varies greatly among species. Several Ulva species have been considered in aquafeed formulations for different farmed fish, and Ulva ohnoi is being applied recently. However, the effects of seaweed dietary inclusion on the host must be evaluated. Considering the important role of the host intestinal microbiota, the potential effects of U. ohnoi dietary inclusion need to be studied. In this study, the characterization of the intestinal microbiome of Solea senegalensis, a flatfish with high potential for aquaculture in South Europe, receiving U. ohnoi (5%)-supplemented diet for 90 days has been carried out. In addition, the functional profiles of bacterial communities have been determined by using PICRUSt, a computational approach to predict the functional composition of a metagenome by using marker gene data and a database of reference genomes. The results show that long-term dietary administration of U. ohnoi (5%)-supplemented feed modulates S. senegalensis intestinal microbiota, especially in the posterior intestinal section. Increased relative abundance of Vibrio jointly with decreased Stenotrophomonas genus has been detected in fish receiving Ulva diet compared to control-fed fish. The influence of the diet on the intestinal functionality of S. senegalensis has been studied for the first time. Changes in bacterial composition were accompanied by differences in predicted microbiota functionality. Increased abundance of predicted genes involved in xenobiotic biodegradation and metabolism were observed in the microbiota when U. ohnoi diet was used. On the contrary, predicted percentages of genes associated to penicillin and cephalosporin biosynthesis as well as beta-lactam resistance were reduced after feeding with Ulva diet.