ABSTRACT
Gender stereotypes and lack of access to relatable role models, mentors, and STEM opportunities have been suggested to deter middle school girls and students from underrepresented backgrounds away from STEM. Seeds of Success, an out-of-school program, is designed to inspire girls to consider STEM careers by countering gender stereotypes through relatable role models, promoting STEM confidence through STEM workshops and hands-on activities, and encouraging alignment between cultural and STEM identities through community-based STEM projects that develop leadership skills. Since 2015, the program has impacted 453 students who in turn have reached more than 42,777 people in Puerto Rico through their STEM Ambassadors projects. A robust mix-method evaluation of the 2020 and 2021 cohorts demonstrates significant improvements in participants' STEM attitudes and science identity, as well as in their self-perception as a leader, confidence in their ability to succeed in science, knowledge about STEM careers and opportunities, and access to STEM role models. Moreover, 95% of participants intend to continue participating in STEM activities after the program and overall scores for the entire survey were significantly higher after the program than before. We discuss lessons learned for other programs seeking to empower girls from historically underserved backgrounds in STEM.
ABSTRACT
Ciencia Puerto Rico, a non-profit organization dedicated to promoting science, research and scientific education among Latinos, organized an educational symposium to provide college science majors the tools, opportunities and advice to pursue graduate degrees and succeed in the STEM disciplines. In this article we share our experiences and lessons learned, for others interested in developing large-scale events to recruit underrepresented minorities to STEM and in evaluating the effectiveness of these efforts.
ABSTRACT
Appropriate targeting of inner nuclear membrane (INM) proteins is important for nuclear function and architecture. To gain new insights into the mechanism(s) for targeting and/or tethering peripherally associated proteins to the INM, we screened a collection of temperature sensitive S. cerevisiae yeast mutants for defects in INM location of the peripheral protein, Trm1-II-GFP. We uncovered numerous genes encoding components of the Spindle Pole Body (SPB), the yeast centrosome. SPB alterations affect the localization of both an integral (Heh2) and a peripheral INM protein (Trm1-II-GFP), but not a nucleoplasmic protein (Pus1). In wild-type cells Trm1-II-GFP is evenly distributed around the INM, but in SPB mutants, Trm1-II-GFP mislocalizes as a spot(s) near ER-nucleus junctions, perhaps its initial contact site with the nuclear envelope. Employing live cell imaging over time in a microfluidic perfusion system to study protein dynamics, we show that both Trm1-II-GFP INM targeting and maintenance depend upon the SPB. We propose a novel targeting and/or tethering model for a peripherally associated INM protein that combines mechanisms of both integral and soluble nuclear proteins, and describe a role of the SPB in nuclear envelope dynamics that affects this process.
